PDB entry 3fhj

View 3fhj on RCSB PDB site
Description: Independent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulations
Class: translation
Keywords: ligand-dependent domain rearrangement, mechanistic pathway, molecular simulations, Aminoacyl-tRNA synthetase, ATP-binding, Cytoplasm, Ligase, Nucleotide-binding, Protein biosynthesis, TRANSLATION
Deposited on 2008-12-09, released 2009-02-03
The last revision prior to the SCOPe 2.01 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.65 Å
R-factor: 0.21
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhja_
  • Chain 'B':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhjb_
  • Chain 'C':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhjc_
  • Chain 'D':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhjd_
  • Chain 'E':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhje_
  • Chain 'F':
    Compound: Tryptophanyl-tRNA synthetase
    Species: Bacillus stearothermophilus [TaxId:1422]
    Gene: trpS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00953 (0-325)
      • conflict (63)
    Domains in SCOPe 2.01: d3fhjf_
  • Heterogens: TRP, PO4, AMP

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3fhjA (A:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjA (A:)
mktifsgiqtignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirrlaaly
lavgidptqatlfiqsevpahaqaawmlqcivyigelermtqvsaglltypplmaadill
yntdivpvgedqkqhieltrdlaerfnkryftipearipkvgarimslvdptkkmsksdp
npkayitllddaktiekkikssegtirypgisnllniystlsgqsieelerqyegkgygv
fkadlaqvvietlrpiqeryhhwmeseeldrvldegaekanrvasemvrkmeqamglgr

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3fhjB (B:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjB (B:)
mktifsgiqtignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirrlaaly
lavgidptqatlfiqsevpahaqaawmlqcivyigelermtqvsaglltypplmaadill
yntdivpvgedqkqhieltrdlaerfnkryftiperimslvdptkkmsksdpnpkayitl
lddaktiekkikssegtirypgisnllniystlsgqsieelerqyegkgygvfkadlaqv
vietlrpiqeryhhwmeseeldrvldegaekanrvasemvrkmeqamglgr

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3fhjC (C:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjC (C:)
mktifsgiqtignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirrlaaly
lavgidptqatlfiqsevpahaqaawmlqcivyigelermtqvsaglltypplmaadill
yntdivpvgedqkqhieltrdlaerfnkryftipearipkvgarimslvdptkkmsksdp
npkayitllddaktiekkikssegtpgisnllniystlgvfkadlaqvvietlrpiqery
hhwmeseeldrvldegaekanrvasemvrkmeqamglgr

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3fhjD (D:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjD (D:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqvsaglltypplma
adillyntdivpvgedqkqhieltrdlaerfftipearipkvgarimslvdptkkmsksd
pnpkayitllddaktiekkiksaisnllniystlsgqsieelerqyvfkadlaqvvietl
rpiqeryhhwmeseeldrvldegaekanrvasemvrkmeqamglgr

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3fhjE (E:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjE (E:)
mktifsgiqtignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirrlaaly
lavgidptqatlfiqsevpahaqaawmlqcivyigelermtqvsaglltypplmaadill
yntdivpvgedqkqhieltrdlaerfnkryftipearipkvgarimslvdptkkmsksdp
npkayitllddaktiekkikspgisnllniystlsgqsieelerqyegkgygvfkadlaq
vvietlrpiqeryhhwmeseeldrvldegaekanrvasemvrkmeqamglgr

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3fhjF (F:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermtqfkeksagkeavsa
glltypplmaadillyntdivpvgedqkqhieltrdlaerfnkrygelftipearipkvg
arimslvdptkkmsksdpnpkayitllddaktiekkiksavtdsegtirydkeakpgisn
llniystlsgqsieelerqyegkgygvfkadlaqvvietlrpiqeryhhwmeseeldrvl
degaekanrvasemvrkmeqamglgrrr

    Sequence, based on observed residues (ATOM records): (download) 
    >3fhjF (F:)
mktifsgiqpsgvitignyigalrqfvelqheyncyfcivdqhaitvwqdphelrqnirr
laalylavgidptqatlfiqsevpahaqaawmlqcivyigelermvsaglltypplmaad
illyntdivpvgedqkqhieltrdlaerfnkryftipearrimslvdptkkmsksdpnpk
ayitllddaktiekkikssegtirypgisnllniystlsgqsieelerqyegkgygvfka
dlaqvvietlrpiqeryhhwmeseeldrvldegaekanrvasemvrkmeqamglgr