PDB entry 3fbd

View 3fbd on RCSB PDB site
Description: Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA
Class: hydrolase/DNA
Keywords: Computational redesign, Protein engineering, Protein-nucleic acid interactions, DNase, DNA hydrolysis., Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Metal-binding, Nuclease, Plasmid, Zinc, HYDROLASE/DNA COMPLEX
Deposited on 2008-11-19, released 2009-11-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-11-03, with a file datestamp of 2009-10-30.
Experiment type: XRAY
Resolution: 2.9 Å
R-factor: 0.201
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

• Chain 'A':
  Compound: Colicin-E7
  Species: ESCHERICHIA COLI [TaxId:562]
  Gene: cea, colE7
  Database cross-references and differences (RAF-indexed):
  • Uniprot Q47112 (0-131)
    • engineered (48)
  Domains in SCOPe 2.08: d3fbda_
• Chain 'B':
  Compound: 5'-d(*dgp*dgp*dap*dap*dtp*dtp*dcp*dgp*dap*dtp*dcp*dgp*dap*dap*dtp*dtp*dcp*dc)-3'
  
• Chain 'C':
  Compound: 5'-d(*dgp*dgp*dap*dap*dtp*dtp*dcp*dgp*dap*dtp*dcp*dgp*dap*dap*dtp*dtp*dcp*dc)-3'
  
• Chain 'D':
  Compound: Colicin-E7
  Species: ESCHERICHIA COLI [TaxId:562]
  Gene: cea, colE7
  Database cross-references and differences (RAF-indexed):
  • Uniprot Q47112 (Start-131)
    • engineered (48)
  Domains in SCOPe 2.08: d3fbdd_
• Chain 'E':
  Compound: 5'-d(*dgp*dgp*dap*dap*dtp*dtp*dcp*dgp*dap*dtp*dcp*dgp*dap*dap*dtp*dtp*dcp*dc)-3'
  
• Chain 'F':
  Compound: 5'-d(*dgp*dgp*dap*dap*dtp*dtp*dcp*dgp*dap*dtp*dcp*dgp*dap*dap*dtp*dtp*dcp*dc)-3'
  
• Heterogens: HOH

PDB Chain Sequences:

• Chain 'A':
  Sequence; same for both SEQRES and ATOM records: (download)
  

  >3fbdA (A:)
  skrnkpgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfqdfrkkfweevs
  kdpelskqfsrnnndrmkvgkapktrtqdvsgkrtsfelhhekpisqnggvydmdnisvv
  tpkrhidihrgk
  

  
  
• Chain 'B':
  No sequence available.
  
  
• Chain 'C':
  No sequence available.
  
  
• Chain 'D':
  Sequence, based on SEQRES records: (download)
  

  >3fbdD (D:)
  skrnkpgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfqdfrkkfweevs
  kdpelskqfsrnnndrmkvgkapktrtqdvsgkrtsfelhhekpisqnggvydmdnisvv
  tpkrhidihrgk
  

  
  Sequence, based on observed residues (ATOM records): (download)
  

  >3fbdD (D:)
  krnkpgkatgkgkpvnnkwlnnagkdlgspvpdrianklrdkefksfqdfrkkfweevsk
  dpelskqfsrnnndrmkvgkapktrtqdvsgkrtsfelhhekpisqnggvydmdnisvvt
  pkrhidihrgk
  

  
  
• Chain 'E':
  No sequence available.
  
  
• Chain 'F':
  No sequence available.