PDB entry 3f1h

View 3f1h on RCSB PDB site
Description: Crystal structure of a translation termination complex formed with release factor RF2. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes as described in remark 400.
Deposited on 2008-10-27, released 2008-12-23
The last revision was dated 2014-12-10, with a file datestamp of 2014-12-05.
Experiment type: XRAY
Resolution: 3 Å
R-factor: 0.281
AEROSPACI score: 0.08 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 50S ribosomal protein L27
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '1':
    Compound: 50S ribosomal protein L28
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '2':
    Compound: 50S ribosomal protein L29
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '3':
    Compound: 50S ribosomal protein L30
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '4':
    Compound: 50S ribosomal protein L35
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '5':
    Compound: 50S ribosomal protein L31
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '6':
    Compound: 50S ribosomal protein L32
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '7':
    Compound: 50S ribosomal protein L33
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain '8':
    Compound: 50S ribosomal protein L34
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q72L77
      • insertion (1-26)
  • Chain 'A':
    Compound: 23s rRNA
    Species: Thermus thermophilus [TaxId:274]
  • Chain 'B':
    Compound: 5s rRNA
    Species: Thermus thermophilus [TaxId:274]
  • Chain 'D':
    Compound: 50S ribosomal protein L2
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: 50S ribosomal protein L3
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: 50S ribosomal protein L4
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: 50S ribosomal protein L5
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: 50S ribosomal protein L6
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: 50S ribosomal protein L9
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: 50S ribosomal protein L11
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: 50S ribosomal protein L13
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: 50S ribosomal protein L14
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'P':
    Compound: 50S ribosomal protein L15
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Q':
    Compound: 50S ribosomal protein L16
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: 50S ribosomal protein L17
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'S':
    Compound: 50S ribosomal protein L18
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'T':
    Compound: 50S ribosomal protein L19
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'U':
    Compound: 50S ribosomal protein L20
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'V':
    Compound: 50S ribosomal protein L21
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'W':
    Compound: 50S ribosomal protein L22
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'X':
    Compound: 50S ribosomal protein L23
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Y':
    Compound: 50S ribosomal protein L24
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Chain 'Z':
    Compound: 50S ribosomal protein L25
    Species: Thermus thermophilus [TaxId:274]
    Database cross-references and differences (RAF-indexed):
  • Heterogens: MG

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain '0':
    Sequence, based on SEQRES records:
    >3f1h0 (0:)
mahkkglgstkngrdsqakrlgvkryegqvvragnilvrqrgtrfkpgknvgmgrdftlf
alvdgvvefqdrgrlgryvhvrpla

    Sequence, based on observed residues (ATOM records):
    >3f1h0 (0:)
tkngrdsqakrlgvkryegqvvragnilvrqrgtrfkpgknvgmgrdftlfalvdgvvef
qdrgrlgryvhvrpla

  • Chain '1':
    Sequence, based on SEQRES records:
    >3f1h1 (1:)
mskvceisgkrpivansiqrrgkakreggvgkkttgiskrrqypnlqkvrvrvagqeitf
rvaashipkvyelverakglrleglspkeikkellkll

    Sequence, based on observed residues (ATOM records):
    >3f1h1 (1:)
sgkrpivansiqrrgkakreggvgkkttgiskrrqypnlqkvrvrvagqeitfrvaashi
pkvyelverakglrleglspkeikkell

  • Chain '2':
    Sequence, based on SEQRES records:
    >3f1h2 (2:)
mklsevrkqleearklspveleklvrekkrelmelrfqasigqlsqnhkirdlkrqiarl
ltvlnekrrqna

    Sequence, based on observed residues (ATOM records):
    >3f1h2 (2:)
mklsevrkqleearklspveleklvrekkrelmelrfqasigqlsqnhkirdlkrqiarl
lt

  • Chain '3':
    Sequence, based on SEQRES records:
    >3f1h3 (3:)
mprlkvklvkspigypkdqkaalkalglrrlqqervledtpairgnvekvahlvrvevve

    Sequence, based on observed residues (ATOM records):
    >3f1h3 (3:)
mprlkvklvkspigypkdqkaalkalglrrlqqervledtpairgnvekvahlvrvevv

  • Chain '4':
    Sequence, based on SEQRES records:
    >3f1h4 (4:)
mplgvhplytkrwlahgqdrakkeanvkegihpklvpariicgcgnvietystkpeiyve
vcskchpfytgqqrfvdtegrverfqrrygdsyrkgr

    Sequence, based on observed residues (ATOM records):
    >3f1h4 (4:)
vpariicgcgnvietystkpeiyvevcskc

  • Chain '5':
    Sequence, based on SEQRES records:
    >3f1h5 (5:)
makhpvpkkktskarrdarrshhaltppilvpcpeckamkpphtvcpecgyyagrkvlev

    Sequence, based on observed residues (ATOM records):
    >3f1h5 (5:)
akhpvpkkktskarrdarrshhaltppilvpcpeckamkpphtvcpecgyya

  • Chain '6':
    Sequence, based on SEQRES records:
    >3f1h6 (6:)
masevriklllecteckrrnyateknkrntpnklelrkycpwcrkhtvhrevki

    Sequence, based on observed residues (ATOM records):
    >3f1h6 (6:)
lllecteckrrnyateknkrntpnklelrkycpwcrkhtvhrev

  • Chain '7':
    Sequence, based on SEQRES records:
    >3f1h7 (7:)
mkrtwqpnrrkrakthgfrarmrtpggrkvlkrrrqkgrwrltpavrkr

    Sequence, based on observed residues (ATOM records):
    >3f1h7 (7:)
mkrtwqpnrrkrakthgfrarmrtpggrkvlkrrrqkgrwrltpavrk

  • Chain '8':
    Sequence, based on SEQRES records:
    >3f1h8 (8:)
mpkmkthkgakkrvkitasgkvvamktgkrhlnwqksgkeirqkgrkfvlakpeaerikl
llpye

    Sequence, based on observed residues (ATOM records):
    >3f1h8 (8:)
pkmkthkgakkrvkitasgkvvamktgkrhlnwqksgkeirqkgrkfvlakpeaerikll
lpy

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3f1hD (D:)
mavkkfkpytpsrrfmtvadfseitktepekslvkplkktggrnnqgritvrfrggghkr
lyriidfkrwdkvgipakvaaieydpnrsariallhyvdgekryiiapdglqvgqqvvag
pdapiqvgnalplrfipvgtvvhavelepkkgaklaraagtsaqiqgregdyvilrlpsg
elrkvhgecyatvgavgnadhknivlgkagrsrwlgrrphvrgaamnpvdhphgggegra
prgrppaspwgwqtkglktrkrrkpssrfiiarrkk

    Sequence, based on observed residues (ATOM records):
    >3f1hD (D:)
avkkfkpytpsrrfmtvadfseitktepekslvkplkktggrnnqgritvrfrggghkrl
yriidfkrwdkvgipakvaaieydpnrsariallhyvdgekryiiapdglqvgqqvvagp
dapiqvgnalplrfipvgtvvhavelepkkgaklaraagtsaqiqgregdyvilrlpsge
lrkvhgecyatvgavgnadhknivlgkagrsrwlgrrphvrgaamnpvdhphgggegrap
rgrppaspwgwqtkglktrkrrkpssrfiia

  • Chain 'E':
    Sequence, based on SEQRES records:
    >3f1hE (E:)
mkgilgvkvgmtrifrddravpvtvilagpcpvvqrrtpekdgytavqlgflpqnpkrvn
rplkghfakagvepvrilreirdfnpegdtvtveifkpgervdvtgtskgrgfagvmkrw
nfaggpdshgahkihrhpgsignrktpgrvykgkkmaghygaervtvmnlevvdvipeen
lllvkgavpgpngglvivretkkaak

    Sequence, based on observed residues (ATOM records):
    >3f1hE (E:)
mkgilgvkvgmtrifrddravpvtvilagpcpvvqrrtpekdgytavqlgflpqnpkrvn
rplkghfakagvepvrilreirdfnpegdtvtveifkpgervdvtgtskgrgfagvmkrw
nfaggpdshgahkihrhpgsignrktpgrvykgkkmaghygaervtvmnlevvdvipeen
lllvkgavpgpngglvivretkka

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3f1hF (F:)
mkevamyqipvlspsgrrelaadlpaeinphllwevvrwqlakrrrgtastktrgevays
grkiwpqkhtgrarhgdigapifvgggvvfgpkprdysytlpkkvrkkglamavadrare
gklllveafagvngktkeflawakeagldgsesvllvtgnelvrraarnlpwvvtlapeg
lnvydivrterlvmdldawevfqnriggea

    Sequence, based on observed residues (ATOM records):
    >3f1hF (F:)
myqipvlspsgrrelaadlpaeinphllwevvrwqlakrrrgtastktrgevaysgrkiw
pqkhtgrarhgdigapifvgggvvfgpkprdysytlpkkvrkkglamavadraregklll
veafagvngktkeflawakeagldgsesvllvtgnelvrraarnlpwvvtlapeglnvyd
ivrterlvmdldawevfqnrig

  • Chain 'G':
    Sequence, based on SEQRES records:
    >3f1hG (G:)
mpldlalkrkyyeevrpelirrfgyqnvwevprlekvvinqglgeakedarilekaaqel
alitgqkpavtrakksisnfklrkgmpiglrvtlrrdrmwiflekllnvalprirdfrgl
npnsfdgrgnynlglreqlifpeitydmvdalrgmdiavvttaetdeearallellgfpf
rk

    Sequence, based on observed residues (ATOM records):
    >3f1hG (G:)
pldlalkrkyyeevrpelirrfgyqnvwevprlekvvinqglgeakedarilekaaqela
litgqkpavtrakksisnfklrkgmpiglrvtlrrdrmwiflekllnvalprirdfrgln
pnsfdgrgnynlglreqlifpeitydmvdalrgmdiavvttaetdeearallellgfpfr
k

  • Chain 'H':
    Sequence, based on SEQRES records:
    >3f1hH (H:)
msrigrlpipvpkgvsvevapgrvkvkgpkgelevpvspemrvvveegvvrverpsderr
hkslhgltrtlianavkgvsegyskellikgigyrarlvgraleltvgfshpvvveppeg
itfevpeptrvrvsgidkqkvgqvaanirairkpsayhekgiyyagepvrlkpgkagakk

    Sequence, based on observed residues (ATOM records):
    >3f1hH (H:)
pkgvsvevapgrvkvkgpkgelevpvspemrvvveegvvrverpsderrhkslhgltrtl
ianavkgvsegyskellikgigyrarlvgraleltvgfshpvvveppegitfevpeptrv
rvsgidkqkvgqvaanirairkpsayhekgiyyagepvr

  • Chain 'I':
    Sequence, based on SEQRES records:
    >3f1hI (I:)
mkvilleplenlgdvgqvvdvkpgyarnyllprglavlatesnlkaleariraqakrlae
rkaeaerlkeilenltltipvragetkiygsvtakdiaealsrqhgitidpkrlalekpi
kelgeyvltykphpevpiqlkvsvvaqe

    Sequence, based on observed residues (ATOM records):
    >3f1hI (I:)
mkvilleplenlgdvgqvvdvkpgyarnyllprglavlatesnlkaleariraqakrlae
rkaeaerlkeilenltltipvragetkiygsvtakdiaealsrqhgitidpkrlalekpi
kelgeyvltykphpevpiqlkvsvv

  • Chain 'K':
    Sequence; same for both SEQRES and ATOM records:
    >3f1hK (K:)
mkkvvavvklqlpagkatpappvgpalgqhganimefvkafnaatanmgdaivpveitiy
adrsftfvtktppasylirkaaglekgahkpgrekvgritweqvleiakqkmpdlnttdl
eaaarmiagsarsmgvevvgapevkda

  • Chain 'N':
    Sequence, based on SEQRES records:
    >3f1hN (N:)
mvksslaflrgppiprqeqrralvktyvpkqveprwvlidaegktlgrlatkiatllrgk
hrpdwtpnvamgdfvvvvnadkirvtgkkleqkiytrysgypgglkkiplekmlathper
vlehavkgmlpkgplgrrlfkrlkvyagpdhphqaqrpeklev

    Sequence, based on observed residues (ATOM records):
    >3f1hN (N:)
ktyvpkqveprwvlidaegktlgrlatkiatllrgkhrpdwtpnvamgdfvvvvnadkir
vtgkkleqkiytrysgypgglkkiplekmlathpervlehavkgmlpkgplgrrlfkrlk
vyagpdhphqaqrpekl

  • Chain 'O':
    Sequence; same for both SEQRES and ATOM records:
    >3f1hO (O:)
miqpqtylevadntgarkimcirvlkgsnakyatvgdvivasvkeaiprgavkegdvvka
vvvrtkkevkrpdgsairfddnaaviinnqleprgtrvfgpvarelrekgfmkivslape
vl

  • Chain 'P':
    Sequence, based on SEQRES records:
    >3f1hP (P:)
mklsdlrpnpgankrrkrvgrgpgsghgktatrghkgqksrsgglkdprrfeggrsttlm
rlpkrgmqgqvpgeikrpryqgvnlkdlarfegevtpellvragllkkgyrlkilgegea
kplkvvahafsksaleklkaaggepvllea

    Sequence, based on observed residues (ATOM records):
    >3f1hP (P:)
dlrpnpgankrrkrvgrgpgsghgktatrghkgqksrsgglkdprrfeggrsttlmrlpk
rgmqgqvpgeikrpryqgvnlkdlarfegevtpellvragllkkgyrlkilgegeakplk
vvahafsksaleklkaaggepvllea

  • Chain 'Q':
    Sequence, based on SEQRES records:
    >3f1hQ (Q:)
mlmprrmkyrkqqrgrlkgatkggdyvafgdfglvalepawitaqqieaarvamvrhfrr
ggkifirifpdkpytkkplevrmgkgkgnvegyvavvkpgrvmfevagvteeqamealri
aghklpiktkivrrdaydeaq

    Sequence, based on observed residues (ATOM records):
    >3f1hQ (Q:)
rmkyrkqqrgrlkgatkggdyvafgdfglvalepawitaqqieaarvamvrhfrrggkif
irifpdkpytkkplevrmgkgkgnvegyvavvkpgrvmfevagvteeqamealriaghkl
piktkivrrdayde

  • Chain 'R':
    Sequence, based on SEQRES records:
    >3f1hR (R:)
mrhlksgrklnrhsshrlalyrnqaksllthgritttvpkakelrgfvdhlihlakrgdl
harrlvlrdlqdvklvrklfdeiapryrdrqggytrvlklaerrrgdgaplalvelve

    Sequence, based on observed residues (ATOM records):
    >3f1hR (R:)
rhlksgrklnrhsshrlalyrnqaksllthgritttvpkakelrgfvdhlihlakrgdlh
arrlvlrdlqdvklvrklfdeiapryrdrqggytrvlklaerrrgdgaplalvelve

  • Chain 'S':
    Sequence, based on SEQRES records:
    >3f1hS (S:)
marltayerrkfrvrnrikrtgrlrlsvfrslkhiyaqiiddekgvtlvsasslalklkg
nktevarqvgralaekalalgikqvafdrgpykyhgrvkalaegaregglef

    Sequence, based on observed residues (ATOM records):
    >3f1hS (S:)
kfrvrnrikrtgrlrlsvfrslkhiyaqiiddekgvtlvsasslalklkgnktevarqvg
ralaekalalgikqvafdrgpykyhgrvkalaegareg

  • Chain 'T':
    Sequence, based on SEQRES records:
    >3f1hT (T:)
mnrgaliklvesryvrtdlpefrpgdtvrvsykvkegnrtriqdfegivirirrngfntt
ftvrkvsygvgverifplhspliqkidivqrgrarraklyfirnlsdreirrklradrkr
idkdraaeraakeevqkaqepeasqe

    Sequence, based on observed residues (ATOM records):
    >3f1hT (T:)
mnrgaliklvesryvrtdlpefrpgdtvrvsykvkegnrtriqdfegivirirrngfntt
ftvrkvsygvgverifplhspliqkidivqrgrarraklyfirnlsdreirrklradrkr
idkdraaeraakeevqk

  • Chain 'U':
    Sequence, based on SEQRES records:
    >3f1hU (U:)
mpraktgvvrrrkhkkilklakgywglrsksfrkaretlfaagnyayahrkrrkrdfrrl
wivrinaacrqhglnystfihglkkagievdrknladlavrepqvfaelverakaaqg

    Sequence, based on observed residues (ATOM records):
    >3f1hU (U:)
praktgvvrrrkhkkilklakgywglrsksfrkaretlfaagnyayahrkrrkrdfrrlw
ivrinaacrqhglnystfihglkkagievdrknladlavrepqvfaelverakaaqg

  • Chain 'V':
    Sequence; same for both SEQRES and ATOM records:
    >3f1hV (V:)
mfaivktggkqyrvepglklrvekldaepgatvelpvlllggektvvgtpvvegasvvae
vlghgrgkkilvskfkakvqyrrkkghrqpytellikeirg

  • Chain 'W':
    Sequence, based on SEQRES records:
    >3f1hW (W:)
meakaiaryvrisprkvrlvvdlirgksleearnilrytnkrgayfvakvlesaaanavn
nhdmledrlyvkaayvdegpalkrvlprargradiikkrtshitvilgekhgk

    Sequence, based on observed residues (ATOM records):
    >3f1hW (W:)
meakaiaryvrisprkvrlvvdlirgksleearnilrytnkrgayfvakvlesaaanavn
nhdmledrlyvkaayvdegpalkrvlprargradiikkrtshitvilgekhg

  • Chain 'X':
    Sequence, based on SEQRES records:
    >3f1hX (X:)
mktaydvilapvlsekayagfaegkytfwvhpkatkteiknavetafkvkvvkvntlhvr
gkkkrlgrylgkrpdrkkaivqvapgqkiealegli

    Sequence, based on observed residues (ATOM records):
    >3f1hX (X:)
taydvilapvlsekayagfaegkytfwvhpkatkteiknavetafkvkvvkvntlhvrgk
kkrlgrylgkrpdrkkaivqvapgqkiealeg

  • Chain 'Y':
    Sequence, based on SEQRES records:
    >3f1hY (Y:)
mrvkmhvkkgdtvlvasgkykgrvgkvkevlpkkyavivegvnivkkavrvspkypqggf
iekeaplhaskvrpicpacgkptrvrkkflengkkirvcakcggaldtee

    Sequence, based on observed residues (ATOM records):
    >3f1hY (Y:)
rvkmhvkkgdtvlvasgkykgrvgkvkevlpkkyavivegvnivkkavrvspkypqggfi
ekeaplhaskvrpicpacgkptrvrkkflengkkirvcak

  • Chain 'Z':
    Sequence, based on SEQRES records:
    >3f1hZ (Z:)
meyrlkayyregekpsalrragklpgvmynrhlnrkvyvdlvefdkvfrqasihhvivle
lpdgqslptlvrqvnldkrrrrpehvdffvlsdepvemyvplrfvgtpagvraggvlqei
hrdilvkvsprnipefievdvsgleigdslhasdlklppgvelavspeetiaavvppedv
eklaeeaaaevaepevikkgkeeeee

    Sequence, based on observed residues (ATOM records):
    >3f1hZ (Z:)
yrlkayyregekpsalrragklpgvmynrhlnrkvyvdlvefdkvfrqasihhvivlelp
dgqslptlvrqvnldkrrrrpehvdffvlsdepvemyvplrfvgtpagvraggvlqeihr
dilvkvsprnipefievdvsgleigdslhasdlklppgvelavspeetiaavvppedvek
laeeaaa