PDB entry 3er8

View 3er8 on RCSB PDB site
Description: Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA
Class: Transcription, Transferase/DNA, RNA
Keywords: Polyadenylate polymerase, translocation, single tranded RNA poly(A) polymerase, RNA protein complex, processivity, heterodimer, nucleotidyltransferase, poxvirus; Methyltransferase, mRNA capping, mRNA processing, S-adenosyl-L-methionine, Transcription, Transferase, Transferase/DNA, RNA COMPLEX, Transferase-DNA
Deposited on 2008-10-01, released 2009-06-16
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-01-24, with a file datestamp of 2018-01-19.
Experiment type: XRAY
Resolution: 3.18 Å
R-factor: N/A
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase
    Species: vaccinia virus WR [TaxId:10254]
    Gene: PAPS, VACWR095, F9
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07617 (0-296)
      • engineered (139)
    Domains in SCOPe 2.08: d3er8a_
  • Chain 'B':
    Compound: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase
    Species: vaccinia virus WR [TaxId:10254]
    Gene: PAPS, VACWR095, F9
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07617 (0-296)
      • engineered (139)
    Domains in SCOPe 2.08: d3er8b_
  • Chain 'C':
    Compound: Poly(A) polymerase catalytic subunit
    Species: vaccinia virus WR [TaxId:10254]
    Gene: PAPL, VACWR057, E1L
    Database cross-references and differences (RAF-indexed):
    • Uniprot P23371 (Start-478)
      • engineered (35)
    Domains in SCOPe 2.08: d3er8c_
  • Chain 'D':
    Compound: Poly(A) polymerase catalytic subunit
    Species: vaccinia virus WR [TaxId:10254]
    Gene: PAPL, VACWR057, E1L
    Database cross-references and differences (RAF-indexed):
    • Uniprot P23371 (Start-478)
      • engineered (35)
    Domains in SCOPe 2.08: d3er8d_
  • Chain 'E':
    Compound: RNA/DNA chimera 5'-D(CP*CP*)R(UP*UP*)D(C)-3'
  • Chain 'F':
    Compound: RNA/DNA chimera 5'-R(P*UP*UP*)D(C)-3'
  • Chain 'G':
    Compound: RNA/DNA chimera 5'-D(P*CP*)R(UP*U)-3'
  • Chain 'H':
    Compound: RNA/DNA chimera 5'-D(P*CP*)R(UP*U)-3'

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3er8A (A:)
mdvvsldkpfmyfeeidneldyepesanevakklpyqgqlklllgelfflsklqrhgild
gatvvyigsapgthirylrdhfynlgviikwmlidgrhhdpilnglrdvtlvtrfvdeey
lrsikkqlhpskiilisdvasaaggnepstadllsnyalqnvmisilnpvasslkwrcpf
pdqwikdfyiphgnkmlqpfapsysaemrllsiytgenmrltrvtksdavnyekkmyyln
kivrnkvvvnfdypnqeydyfhmyfmlrtvycnktfpttkakvlflqqsifrflnip

    Sequence, based on observed residues (ATOM records): (download) 
    >3er8A (A:)
mdvvsldkpfmyfeeidneldyepesklpyqgqlklllgelfflsklqrhgildgatvvy
igsapgthirylrdhfynlgviikwmlidgrhhdpilnglrdvtlvtrfvdeeylrsikk
qlhpskiilisdvasgnepstadllsnyalqnvmisilnpvasslkwrcpfpdqwikdfy
iphgnkmlqpfapsysaemrllsiytgenmrltrvtksdavnyekkmyylnkivrnkvvv
nfdypnqeydyfhmyfmlrtvycnktfpttkakvlflqqsifrflnip

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3er8B (B:)
mdvvsldkpfmyfeeidneldyepesanevakklpyqgqlklllgelfflsklqrhgild
gatvvyigsapgthirylrdhfynlgviikwmlidgrhhdpilnglrdvtlvtrfvdeey
lrsikkqlhpskiilisdvasaaggnepstadllsnyalqnvmisilnpvasslkwrcpf
pdqwikdfyiphgnkmlqpfapsysaemrllsiytgenmrltrvtksdavnyekkmyyln
kivrnkvvvnfdypnqeydyfhmyfmlrtvycnktfpttkakvlflqqsifrflnip

    Sequence, based on observed residues (ATOM records): (download) 
    >3er8B (B:)
mdvvsldkpfmyfeeidneldyepesklpyqgqlklllgelfflsklqrhgildgatvvy
igsapgthirylrdhfynlgviikwmlidgrhhdpilnglrdvtlvtrfvdeeylrsikk
qlhpskiilisdvasgnepstadllsnyalqnvmisilnpvasslkwrcpfpdqwikdfy
iphgnkmlqpfapsysaemrllsiytgenmrltrvtksdavnyekkmyylnkivrnkvvv
nfdypnqeydyfhmyfmlrtvycnktfpttkakvlflqqsifrflnip

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3er8C (C:)
mnrnpdqntlpnitlkiietylgrvpsvneyhmlksqarniqkitvfnkdifvslvkknk
krffsdvntsaseikdrilsyfskqtqtynigklftiielqsvlvttytdilgvltikap
nvisskisynvtsmeelardmlnsmnvavidkakvmgrhnvsslvknvnklmeeylrrhn
kscicygsyslylinpnirygdidilqtnsrtflidlaflikfitgnniilskipylrny
mvikdendnhiidsfnirqdtmnvvpkifidniyivdptfqllnmikmfsqidrledlsk
dpekfnarmatmleyvrythgivfdgkrnnmpmkciidennrivtvttkdyfsfkkclvy
ldenvlssdildlnadtscdfesvtnsvylihdnimytyfsntillsdkgkvheisargl
cahillyqmltsgeykqclsdllnsmmnrdkipiyshterdkkpgrhgfiniekdiivf

    Sequence, based on observed residues (ATOM records): (download) 
    >3er8C (C:)
nitlkiietylgrvpsvneyhmlksqarniqkitvfnkdifvslvkknkkrffsdvntsa
seikdrilsyfskqtqtynigklftiielqsvlvttytdilgvltinvtsmeelardmln
smnvavvsslvknvnklmeeylrrhnkscicygsyslylinpnirygdidilqtnsrtfl
idlaflikfitgnniilskipylrnymvikdendnhiidsfnirqdtmnvvpkifidniy
ivdptfqllnmikmfsqidrledlskdpekfnarmatmleyvrythgivfdgkrnnmpmk
ciidennrivtvttkdyfsfkkclvyldenvlssdildlnadtscdfesvtnsvylihdn
imytyfsntillsdkgkvheisarglcahillyqmltsgeykqclsdllnsmmnrdkipi
yshterdkkpgrhgfiniekdiivf

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3er8D (D:)
mnrnpdqntlpnitlkiietylgrvpsvneyhmlksqarniqkitvfnkdifvslvkknk
krffsdvntsaseikdrilsyfskqtqtynigklftiielqsvlvttytdilgvltikap
nvisskisynvtsmeelardmlnsmnvavidkakvmgrhnvsslvknvnklmeeylrrhn
kscicygsyslylinpnirygdidilqtnsrtflidlaflikfitgnniilskipylrny
mvikdendnhiidsfnirqdtmnvvpkifidniyivdptfqllnmikmfsqidrledlsk
dpekfnarmatmleyvrythgivfdgkrnnmpmkciidennrivtvttkdyfsfkkclvy
ldenvlssdildlnadtscdfesvtnsvylihdnimytyfsntillsdkgkvheisargl
cahillyqmltsgeykqclsdllnsmmnrdkipiyshterdkkpgrhgfiniekdiivf

    Sequence, based on observed residues (ATOM records): (download) 
    >3er8D (D:)
nitlkiietylgrvpsvneyhmlksqarniqkitvfnkdifvslvkknkkrffsdvntsa
seikdrilsyfskqtqtynigklftiielqsvlvttytdilgvltinvtsmeelardmln
smnvavvsslvknvnklmeeylrrhnkscicygsyslylinpnirygdidilqtnsrtfl
idlaflikfitgnniilskipylrnymvikdendnhiidsfnirqdtmnvvpkifidniy
ivdptfqllnmikmfsqidrledlskdpekfnarmatmleyvrythgivfdgkrnnmpmk
ciidennrivtvttkdyfsfkkclvyldenvlssdildlnadtscdfesvtnsvylihdn
imytyfsntillsdkgkvheisarglcahillyqmltsgeykqclsdllnsmmnrdkipi
yshterdkkpgrhgfiniekdiivf

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.