PDB entry 3ekh

View 3ekh on RCSB PDB site
Description: calcium-saturated gcamp2 t116v/k378w mutant monomer
Deposited on 2008-09-19, released 2008-12-16
The last revision was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.32 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Myosin light chain kinase, Green fluorescent protein, Calmodulin chimera
    Species: Rattus norvegicus [TaxId:10116]
    Gene: Calm1, Calm, Cam, Cam1, CaMI
    Database cross-references and differences (RAF-indexed):
    • PDB 3EKH (Start-60)
    • Uniprot P42212 (61-End)
      • see remark 999 (75)
      • see remark 999 (87)
      • see remark 999 (92)
      • engineered mutation (115)
      • see remark 999 (118)
      • see remark 999 (143)
      • linker (158)
    • Uniprot P42212 (159-301)
      • see remark 999 (221)
      • chromophore (223)
      • see remark 999 (250)
      • linker (302-303)
    • Uniprot P0DP29 (304-End)
      • engineered mutation (377)
  • Heterogens: GOL, CA, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3ekhA (A:)
mrgshhhhhhgmasmtggqqmgrdlyddddkdlatmvdssrrkwnktghavraigrlssl
envyimadkqkngikanfkirhniedggvqlayhyqqntpigdgpvllpdnhylsvqskl
skdpnekrdhmvllefvtaagitlgmdelykggtggsmvskgeelftgvvpilveldgdv
nghkfsvsgegegdatygkltlkficttgklpvpwptlvttlgygvqcfsrypdhmkqhd
ffksampegyiqertiffkddgnyktraevkfegdtlvnrielkgidfkedgnilghkle
yntrdqlteeqiaefkeafslfdkdgdgtittkelgtvmrslgqnpteaelqdminevda
dgngtidfpefltmmarwmkdtdseeeireafrvfdkdgngyisaaelrhvmtnlgeklt
deevdemireadidgdgqvnyeefvqmmtak

    Sequence, based on observed residues (ATOM records):
    >3ekhA (A:)
ssrrkwnktghavraigrlsslenvyimadkqkngikanfkirhniedggvqlayhyqqn
tpigdgpvllpdnhylsvqsklskdpnekrdhmvllefvtaagitlvskgeelftgvvpi
lveldgdvnghkfsvsgegegdatygkltlkficttgklpvpwptlvttlgygvqcfsry
pdhmkqhdffksampegyiqertiffkddgnyktraevkfegdtlvnrielkgidfkedg
nilghkleyntrdqlteeqiaefkeafslfdkdgdgtittkelgtvmrslgqnpteaelq
dminevdadgngtidfpefltmmarwmkdtdseeeireafrvfdkdgngyisaaelrhvm
tnlgekltdeevdemireadidgdgqvnyeefvqmmt