PDB entry 3eje

View 3eje on RCSB PDB site
Description: Crystal Structure of P450BioI in complex with octadec-9Z-enoic acid ligated Acyl Carrier Protein
Class: oxidoreductase/lipid transport
Keywords: Protein-Protein Complex, Cytochrome P450 Fold, Carrier Protein, 4-Helix Bundle, Cytoplasm, Fatty acid biosynthesis, Lipid synthesis, Phosphopantetheine, Biotin biosynthesis, Heme, Iron, Metal-binding, Monooxygenase, Oxidoreductase, OXIDOREDUCTASE/LIPID TRANSPORT COMPLEX
Deposited on 2008-09-18, released 2008-10-07
The last revision prior to the SCOPe 2.06 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: 0.242
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Acyl carrier protein
    Species: Escherichia coli [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6A8 (19-End)
      • expression tag (17-18)
    Domains in SCOPe 2.06: d3ejea2, d3ejea3
  • Chain 'B':
    Compound: Biotin biosynthesis cytochrome P450-like enzyme
    Species: Bacillus subtilis [TaxId:1423]
    Gene: bioI, CYP107H, BSU30190
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3ejeb_
  • Chain 'C':
    Compound: Acyl carrier protein
    Species: Escherichia coli [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6A8 (19-End)
      • expression tag (17-18)
    Domains in SCOPe 2.06: d3ejec2, d3ejec3
  • Chain 'D':
    Compound: Biotin biosynthesis cytochrome P450-like enzyme
    Species: Bacillus subtilis [TaxId:1423]
    Gene: bioI, CYP107H, BSU30190
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3ejed_
  • Chain 'E':
    Compound: Acyl carrier protein
    Species: Escherichia coli [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6A8 (19-End)
      • expression tag (17-18)
    Domains in SCOPe 2.06: d3ejee2, d3ejee3
  • Chain 'F':
    Compound: Biotin biosynthesis cytochrome P450-like enzyme
    Species: Bacillus subtilis [TaxId:1423]
    Gene: bioI, CYP107H, BSU30190
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3ejef_
  • Chain 'G':
    Compound: Acyl carrier protein
    Species: Escherichia coli [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6A8 (19-End)
      • expression tag (17-18)
    Domains in SCOPe 2.06: d3ejeg2, d3ejeg3
  • Chain 'H':
    Compound: Biotin biosynthesis cytochrome P450-like enzyme
    Species: Bacillus subtilis [TaxId:1423]
    Gene: bioI, CYP107H, BSU30190
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d3ejeh_
  • Heterogens: ZMO, HTG, HEM, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ejeA (A:)
gsshhhhhhssglvprgshmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtv
elvmaleeefdteipdeeaekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeA (A:)
shmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipde
eaekittvqaaidyingh

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ejeB (B:)
tiasstasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrt
plpesstkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlld
qvqgkkkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkalteg
nimavqamayfkeliqkrkrhpqqdmismllkgrekdklteeeaastcillaiaghettv
nlisnsvlcllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtir
qgeqvylllgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaint
llqrmpslnladfewryrplfgfraleelpvtfeaswshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeB (B:)
seflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrtplpesstk
yqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlldqvqgkkkm
evisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkaltegnimavqam
ayfkeliqkrkrhpqqdmismllkklteeeaastcillaiaghettvnlisnsvlcllqh
peqllklrenpdligtaveeclryesptqmtarvasedidicgvtirqgeqvylllgaan
rdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaintllqrmpslnlawr
yrplfgfraleelpvtfe

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ejeC (C:)
gsshhhhhhssglvprgshmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtv
elvmaleeefdteipdeeaekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeC (C:)
shmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipde
eaekittvqaaidyingh

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3ejeD (D:)
tiasstasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrt
plpesstkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlld
qvqgkkkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkalteg
nimavqamayfkeliqkrkrhpqqdmismllkgrekdklteeeaastcillaiaghettv
nlisnsvlcllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtir
qgeqvylllgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaint
llqrmpslnladfewryrplfgfraleelpvtfeaswshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeD (D:)
tasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrtplpes
stkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlldqvqgk
kkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkaltegnimav
qamayfkeliqkrkrhpqqdmismllkgrklteeeaastcillaiaghettvnlisnsvl
cllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtirqgeqvyll
lgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaintllqrmpsl
nladfwryrplfgfraleelpvtfe

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >3ejeE (E:)
gsshhhhhhssglvprgshmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtv
elvmaleeefdteipdeeaekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeE (E:)
shmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipde
eaekittvqaaidyin

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >3ejeF (F:)
tiasstasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrt
plpesstkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlld
qvqgkkkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkalteg
nimavqamayfkeliqkrkrhpqqdmismllkgrekdklteeeaastcillaiaghettv
nlisnsvlcllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtir
qgeqvylllgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaint
llqrmpslnladfewryrplfgfraleelpvtfeaswshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeF (F:)
seflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrtplpesstk
yqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlldqvqgkkkm
evisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkaltegnimavqam
ayfkeliqkrkrhpqqdmismllkklteeeaastcillaiaghettvnlisnsvlcllqh
peqllklrenpdligtaveeclryesptqmtarvasedidicgvtirqgeqvylllgaan
rdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaintllqrmpslnlade
wryrplfgfraleelpvtfe

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >3ejeG (G:)
gsshhhhhhssglvprgshmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtv
elvmaleeefdteipdeeaekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeG (G:)
shmstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipde
eaekittvqaaidyi

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >3ejeH (H:)
tiasstasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrt
plpesstkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlld
qvqgkkkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkalteg
nimavqamayfkeliqkrkrhpqqdmismllkgrekdklteeeaastcillaiaghettv
nlisnsvlcllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtir
qgeqvylllgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaint
llqrmpslnladfewryrplfgfraleelpvtfeaswshpqfek

    Sequence, based on observed residues (ATOM records): (download) 
    >3ejeH (H:)
tasseflknpysfydtlravhpiykgsflkypgwyvtgyeetaailkdarfkvrtplpes
stkyqdlshvqnqmmlfqnqpdhrrlrtlasgaftprttesyqpyiietvhhlldqvqgk
kkmevisdfafplasfvianiigvpeedreqlkewaasliqtidftrsrkaltegnimav
qamayfkeliqkrkrhpqqdmismllkkdklteeeaastcillaiaghettvnlisnsvl
cllqhpeqllklrenpdligtaveeclryesptqmtarvasedidicgvtirqgeqvyll
lgaanrdpsiftnpdvfditrspnphlsfghghhvclgsslarleaqiaintllqrmpsl
nlawryrplfgfraleelpvtfe