PDB entry 3ecj

View 3ecj on RCSB PDB site
Description: Structure of E323L mutant of Homoprotocatechuate 2,3-Dioxygenase from Brevibacterium fuscum at 1.65A resolution
Class: oxidoreductase
Keywords: oxidoreductase, oxygenase, extradiol, FeII, crystal packing, Dioxygenase
Deposited on 2008-08-31, released 2008-10-07
The last revision prior to the SCOP 1.75 freeze date was dated 2008-10-28, with a file datestamp of 2008-10-24.
Experiment type: XRAY
Resolution: 1.65 Å
R-factor: 0.171
AEROSPACI score: 0.69 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: PROTEIN (Homoprotocatechuate 2,3-dioxygenase)
    Species: Brevibacterium fuscum [TaxId:47914]
    Gene: hpcd
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q45135
      • engineered (322)
    Domains in SCOP 1.75: d3ecja1, d3ecja2
  • Chain 'B':
    Compound: PROTEIN (Homoprotocatechuate 2,3-dioxygenase)
    Species: Brevibacterium fuscum [TaxId:47914]
    Gene: hpcd
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q45135
      • engineered (322)
    Domains in SCOP 1.75: d3ecjb1, d3ecjb2
  • Chain 'C':
    Compound: PROTEIN (Homoprotocatechuate 2,3-dioxygenase)
    Species: Brevibacterium fuscum [TaxId:47914]
    Gene: hpcd
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q45135
      • engineered (322)
    Domains in SCOP 1.75: d3ecjc1, d3ecjc2
  • Chain 'D':
    Compound: PROTEIN (Homoprotocatechuate 2,3-dioxygenase)
    Species: Brevibacterium fuscum [TaxId:47914]
    Gene: hpcd
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q45135
      • engineered (322)
    Domains in SCOP 1.75: d3ecjd1, d3ecjd2
  • Heterogens: FE2, CL, GOL, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ecjA (A:)
msneipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefi
hhnlvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrved
plgfpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvted
iqddegttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdr
iergpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpv
vpswyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfk
lgnql

    Sequence, based on observed residues (ATOM records): (download) 
    >3ecjA (A:)
neipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefihh
nlvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrvedpl
gfpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvtediq
ddegttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdrie
rgpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpvvp
swyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfklg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ecjB (B:)
msneipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefi
hhnlvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrved
plgfpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvted
iqddegttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdr
iergpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpv
vpswyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfk
lgnql

    Sequence, based on observed residues (ATOM records): (download) 
    >3ecjB (B:)
eipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefihhn
lvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrvedplg
fpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvtediqd
degttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdrier
gpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpvvps
wyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfklg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ecjC (C:)
msneipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefi
hhnlvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrved
plgfpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvted
iqddegttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdr
iergpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpv
vpswyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfk
lgnql

    Sequence, based on observed residues (ATOM records): (download) 
    >3ecjC (C:)
eipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefihhn
lvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrvedplg
fpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvtediqd
degttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdrier
gpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpvvps
wyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfklg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3ecjD (D:)
msneipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefi
hhnlvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrved
plgfpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvted
iqddegttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdr
iergpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpv
vpswyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfk
lgnql

    Sequence, based on observed residues (ATOM records): (download) 
    >3ecjD (D:)
eipkpvapapdilrcayaelvvtdlaksrnfyvdvlglhvsyedenqiylrsfeefihhn
lvltkgpvaalkamafrvrtpedvdkaeayyqelgcrterrkdgfvkgigdalrvedplg
fpyefffetthverlhmrydlysagelvrldhfnqvtpdvprgrkyledlgfrvtediqd
degttyaawmhrkgtvhdtaltggngprlhhvafsthekhniiqicdkmgalrisdrier
gpgrhgvsnafylyildpdnhrieiytqdyytgdpdnptitwnvhdnqrrdwwgnpvvps
wyteaskvldldgnvqeiilrtddselevtigadgfsftragdedgsyhgqaskgfklg