PDB entry 3ea3

View 3ea3 on RCSB PDB site
Description: Crystal Structure of the Y246S/Y247S/Y248S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis
Class: lyase
Keywords: phosphatidylinositol-specific phospholipase C, PI-PLC, Dimer, interfacially impaired, membrane binding, TIM barrel, Lipid degradation, Lyase, Secreted
Deposited on 2008-08-24, released 2009-04-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 1.78 Å
R-factor: N/A
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 1-phosphatidylinositol phosphodiesterase
    Species: BACILLUS THURINGIENSIS [TaxId:1428]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P08954 (0-End)
      • engineered (245-247)
      • engineered (250)
    Domains in SCOPe 2.08: d3ea3a_
  • Chain 'B':
    Compound: 1-phosphatidylinositol phosphodiesterase
    Species: BACILLUS THURINGIENSIS [TaxId:1428]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P08954 (0-End)
      • engineered (245-247)
      • engineered (250)
    Domains in SCOPe 2.08: d3ea3b_
  • Heterogens: MN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3ea3A (A:)
    assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
    hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
    mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
    netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
    awnspsssassinpeiandikqknptrvgwviqdyinekwspllyqevirankslike
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ea3A (A:)
    assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
    hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
    mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
    netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
    awnspsssassinpeiandikqknptrvgwviqdyinekwspllyqeviranksli
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3ea3B (B:)
    assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
    hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
    mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
    netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
    awnspsssassinpeiandikqknptrvgwviqdyinekwspllyqevirankslike
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ea3B (B:)
    assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
    hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
    mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
    netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
    awnspsssassinpeiandikqknptrvgwviqdyinekwspllyqeviranksli