PDB entry 3ea1

View 3ea1 on RCSB PDB site
Description: Crystal Structure of the Y247S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis
Class: lyase
Keywords: phosphatidylinositol-specific phospholipase C, PI-PLC, Dimer, interfacially impaired, membrane binding, TIM barrel, Lipid degradation, Lyase, Secreted
Deposited on 2008-08-24, released 2009-04-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 1.75 Å
R-factor: N/A
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 1-phosphatidylinositol phosphodiesterase
    Species: BACILLUS THURINGIENSIS [TaxId:1428]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P08954 (0-End)
      • engineered (246)
      • engineered (250)
    Domains in SCOPe 2.08: d3ea1a_
  • Chain 'B':
    Compound: 1-phosphatidylinositol phosphodiesterase
    Species: BACILLUS THURINGIENSIS [TaxId:1428]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P08954 (0-End)
      • engineered (246)
      • engineered (250)
    Domains in SCOPe 2.08: d3ea1b_
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ea1A (A:)
assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
awnspysyassinpeiandikqknptrvgwviqdyinekwspllyqevirankslike

    Sequence, based on observed residues (ATOM records): (download) 
    >3ea1A (A:)
assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
awnspysyassinpeiandikqknptrvgwviqdyinekwspllyqeviranksli

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ea1B (B:)
assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
awnspysyassinpeiandikqknptrvgwviqdyinekwspllyqevirankslike

    Sequence, based on observed residues (ATOM records): (download) 
    >3ea1B (B:)
assvnelenwskwmqpipdniplarisipgthdsgtfklqnpikqvwgmtqeydfryqmd
hgarifdirgrltddntivlhhgplylyvtlhefineakqflkdnpsetiimslkkeyed
mkgaegsfsstfeknyfvdpiflktegniklgdargkivllkrysgsnesggynnfywpd
netftttvnqnvnvtvqdkykvnydekvksikdtmdetmnnsedlnhlyinftslssggt
awnspysyassinpeiandikqknptrvgwviqdyinekwspllyqeviranksli