PDB entry 3e7j

View 3e7j on RCSB PDB site
Description: heparinaseii h202a/y257a double mutant complexed with a heparan sulfate tetrasaccharide substrate
Deposited on 2008-08-18, released 2008-12-30
The last revision was dated 2021-10-20, with a file datestamp of 2021-10-15.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: heparinase II protein
    Species: Pedobacter heparinus [TaxId:984]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46080 (0-748)
      • engineered mutation (178)
      • engineered mutation (233)
      • see remark 999 (734)
  • Chain 'B':
    Compound: heparinase II protein
    Species: Pedobacter heparinus [TaxId:984]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46080 (0-748)
      • engineered mutation (178)
      • engineered mutation (233)
      • see remark 999 (734)
  • Heterogens: ZN, ACT, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3e7jA (A:)
ysqtkadvvwkdvdgvsmpippkthprlylreqqvpdlknrmndpklkkvwadmikmqed
wkpadipevkdfrfyfnqkgltvrvelmalnylmtkdpkvgreaitsiidtletatfkpa
gdisrgiglfmvtgaivydwcydqlkpeektrfvkafvrlakmlecgyppvkdksivgaa
sewmimrdllsvgiaiydefpemynlaagrffkehlvarnwfypshnyhqgmsalnvrft
ndlfalwildrmgagnvfnpgqqfilydaiykrrpdgqilaggdvdysrkkpkyytmpal
lagsyykdeylnyeflkdpnvephcklfeflwrdtqlgsrkpddlplsrysgspfgwmia
rtgwgpesviaemkvneysflnhqhqdagafqiyykgplaidagsytgssggynsphnkn
ffkrtiahnslliydpketfsssgyggsdhtdfaandggqrlpgkgwiaprdlkemlagd
frtgkilaqgfgpdnqtpdytylkgditaaysakvkevkrsflflnlkdakvpaamivfd
kvvasnpdfkkfwllhsieqpeikgnqitikrtkngdsgmlvntallpdaansnitsigg
kgkdfwvfgtnytndpkpgtdealergewrveitpkkaaaedyylnviqiadntqqklhe
vkridgdkvvgvqladrivtfsktsetvdrpfgfsvvgkgtfkfvmtdllpgtwqvlkdg
kilypalsakgddgalyfegtegtyrflr

    Sequence, based on observed residues (ATOM records):
    >3e7jA (A:)
dvvwkdvdgvsmpippkthprlylreqqvpdlknrmndpklkkvwadmikmqedwkpadi
pevkdfrfyfnqkgltvrvelmalnylmtkdpkvgreaitsiidtletatfkpagdisrg
iglfmvtgaivydwcydqlkpeektrfvkafvrlakmlecgyppvkdksivgaasewmim
rdllsvgiaiydefpemynlaagrffkehlvarnwfypshnyhqgmsalnvrftndlfal
wildrmgagnvfnpgqqfilydaiykrrpdgqilaggdvdysrkkpkyytmpallagsyy
kdeylnyeflkdpnvephcklfeflwrdtqlgsrkpddlplsrysgspfgwmiartgwgp
esviaemkvneysflnhqhqdagafqiyykgplaidagsytgssggynsphnknffkrti
ahnslliydpketfsssgyggsdhtdfaandggqrlpgkgwiaprdlkemlagdfrtgki
laqgfgpdnqtpdytylkgditaaysakvkevkrsflflnlkdakvpaamivfdkvvasn
pdfkkfwllhsieqpeikgnqitikrtkngdsgmlvntallpdaansnitsiggkgkdfw
vfgtnytndpkpgtdealergewrveitpkkaaaedyylnviqiadntqqklhevkridg
dkvvgvqladrivtfsktsetvdrpfgfsvvgkgtfkfvmtdllpgtwqvlkdgkilypa
lsakgddgalyfegtegtyrflr

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3e7jB (B:)
ysqtkadvvwkdvdgvsmpippkthprlylreqqvpdlknrmndpklkkvwadmikmqed
wkpadipevkdfrfyfnqkgltvrvelmalnylmtkdpkvgreaitsiidtletatfkpa
gdisrgiglfmvtgaivydwcydqlkpeektrfvkafvrlakmlecgyppvkdksivgaa
sewmimrdllsvgiaiydefpemynlaagrffkehlvarnwfypshnyhqgmsalnvrft
ndlfalwildrmgagnvfnpgqqfilydaiykrrpdgqilaggdvdysrkkpkyytmpal
lagsyykdeylnyeflkdpnvephcklfeflwrdtqlgsrkpddlplsrysgspfgwmia
rtgwgpesviaemkvneysflnhqhqdagafqiyykgplaidagsytgssggynsphnkn
ffkrtiahnslliydpketfsssgyggsdhtdfaandggqrlpgkgwiaprdlkemlagd
frtgkilaqgfgpdnqtpdytylkgditaaysakvkevkrsflflnlkdakvpaamivfd
kvvasnpdfkkfwllhsieqpeikgnqitikrtkngdsgmlvntallpdaansnitsigg
kgkdfwvfgtnytndpkpgtdealergewrveitpkkaaaedyylnviqiadntqqklhe
vkridgdkvvgvqladrivtfsktsetvdrpfgfsvvgkgtfkfvmtdllpgtwqvlkdg
kilypalsakgddgalyfegtegtyrflr

    Sequence, based on observed residues (ATOM records):
    >3e7jB (B:)
dvvwkdvdgvsmpippkthprlylreqqvpdlknrmndpklkkvwadmikmqedwkpadi
pevkdfrfyfnqkgltvrvelmalnylmtkdpkvgreaitsiidtletatfkpagdisrg
iglfmvtgaivydwcydqlkpeektrfvkafvrlakmlecgyppvkdksivgaasewmim
rdllsvgiaiydefpemynlaagrffkehlvarnwfypshnyhqgmsalnvrftndlfal
wildrmgagnvfnpgqqfilydaiykrrpdgqilaggdvdysrkkpkyytmpallagsyy
kdeylnyeflkdpnvephcklfeflwrdtqlgsrkpddlplsrysgspfgwmiartgwgp
esviaemkvneysflnhqhqdagafqiyykgplaidagsytgssggynsphnknffkrti
ahnslliydpketfsssgyggsdhtdfaandggqrlpgkgwiaprdlkemlagdfrtgki
laqgfgpdnqtpdytylkgditaaysakvkevkrsflflnlkdakvpaamivfdkvvasn
pdfkkfwllhsieqpeikgnqitikrtkngdsgmlvntallpdaansnitsiggkgkdfw
vfgtnytndpkpgtdealergewrveitpkkaaaedyylnviqiadntqqklhevkridg
dkvvgvqladrivtfsktsetvdrpfgfsvvgkgtfkfvmtdllpgtwqvlkdgkilypa
lsakgddgalyfegtegtyrflr