PDB entry 3e5s
View 3e5s on RCSB PDB site
Description: Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature
Class: hydrolase
Keywords: Staphylococcal nuclease, hyperstable variant, hydrolase, pdtp, Calcium, Endonuclease, Membrane, Metal-binding, Nuclease, Secreted, Zymogen
Deposited on
2008-08-14, released
2008-11-04
The last revision prior to the SCOPe 2.06 freeze date was dated
2009-02-24, with a file datestamp of
2009-03-01.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.167
AEROSPACI score: 0.48
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: Thermonuclease
Species: Staphylococcus aureus [TaxId:1280]
Gene: nuc
Database cross-references and differences (RAF-indexed):
- Uniprot P00644
- engineered (43-44)
- engineered (96)
- engineered (110)
- engineered (117)
- engineered (121)
Domains in SCOPe 2.06: d3e5sa_ - Chain 'B':
Compound: Thermonuclease
Species: Staphylococcus aureus [TaxId:1280]
Gene: nuc
Database cross-references and differences (RAF-indexed):
- Uniprot P00644
- engineered (43-44)
- engineered (96)
- engineered (110)
- engineered (117)
- engineered (121)
Domains in SCOPe 2.06: d3e5sb_ - Heterogens: THP, CA, HOH
PDB Chain Sequences:
- Chain 'A':
Sequence, based on SEQRES records: (download)
>3e5sA (A:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasaftkkmv
enakkievefdkgqrtdkygrglayiyadgkmvneakvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq
Sequence, based on observed residues (ATOM records): (download)
>3e5sA (A:)
lhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasaftkkmvenakki
evefdkgqrtdkygrglayiyadgkmvneakvrqglakvayvykgnntheqllrkaeaqa
kkeklniws
- Chain 'B':
Sequence, based on SEQRES records: (download)
>3e5sB (B:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasaftkkmv
enakkievefdkgqrtdkygrglayiyadgkmvneakvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq
Sequence, based on observed residues (ATOM records): (download)
>3e5sB (B:)
lhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasaftkkmvenakki
evefdkgqrtdkygrglayiyadgkmvneakvrqglakvayvykgnntheqllrkaeaqa
kkeklniws