PDB entry 3e42

View 3e42 on RCSB PDB site
Description: Q138F HincII bound to GTCGAC and Ca2+ (cocrystallized)
Class: hydrolase/DNA
Keywords: Protein-DNA Interaction, Endonuclease, Restriction Endonuclease, Indirect Readout, Hydrolase, Nuclease, Restriction system, HYDROLASE/DNA COMPLEX
Deposited on 2008-08-08, released 2008-08-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-01-20, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2.68 Å
R-factor: 0.192
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Type-2 restriction enzyme HindII
    Species: Haemophilus influenzae [TaxId:727]
    Gene: HINDIIR, HI0512
    Database cross-references and differences (RAF-indexed):
    • Uniprot P44413 (0-End)
      • conflict (65)
      • engineered (136)
    Domains in SCOPe 2.08: d3e42a_
  • Chain 'B':
    Compound: Type-2 restriction enzyme HindII
    Species: Haemophilus influenzae [TaxId:727]
    Gene: HINDIIR, HI0512
    Database cross-references and differences (RAF-indexed):
    • Uniprot P44413 (0-End)
      • conflict (65)
      • engineered (136)
    Domains in SCOPe 2.08: d3e42b_
  • Chain 'E':
    Compound: 5'-d(*dgp*dcp*dcp*dgp*dgp*dtp*dcp*dgp*dap*dcp*dcp*dgp*dgp*dc)-3'
  • Chain 'F':
    Compound: 5'-d(*dgp*dcp*dcp*dgp*dgp*dtp*dcp*dgp*dap*dcp*dcp*dgp*dgp*dc)-3'
  • Heterogens: NA, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3e42A (A:)
    sfikpiyqdinsiligqkvkrpksgtlsghaagepfeklvykflkenlsdltfkqyeyln
    dlfmknpaiighearyklfnsptllfllsrgkaatenwsienlfeekqndtadillvkdq
    fyelldvktrnisksafapniisayklaqtcakmidnkefdlfdinylevdwelngedlv
    cvstsfaelfksepselyinwaaamqiqfhvrdldqgfngtreewaksylkhfvtqaeqr
    aismidkfvkpfkkyil
    

    Sequence, based on observed residues (ATOM records): (download)
    >3e42A (A:)
    sfikpiyqdinsiligqkvkrpaagepfeklvykflkenlsdltfkqyeylndlfmknpa
    iighearyklfnsptllfllsrgkaatenwsienlfeekqndtadillvkdqfyelldvk
    trnisksafapniisayklaqtcakmidnkefdlfdinylevdwelngedlvcvstsfae
    lfksepselyinwaaamqiqfhvrdldqgfngtreewaksylkhfvtqaeqraismidkf
    vkpfkkyi
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3e42B (B:)
    sfikpiyqdinsiligqkvkrpksgtlsghaagepfeklvykflkenlsdltfkqyeyln
    dlfmknpaiighearyklfnsptllfllsrgkaatenwsienlfeekqndtadillvkdq
    fyelldvktrnisksafapniisayklaqtcakmidnkefdlfdinylevdwelngedlv
    cvstsfaelfksepselyinwaaamqiqfhvrdldqgfngtreewaksylkhfvtqaeqr
    aismidkfvkpfkkyil
    

    Sequence, based on observed residues (ATOM records): (download)
    >3e42B (B:)
    sfikpiyqdinsiligqkepfeklvykflkenlsdltfkqyeylndlfmknpaiighear
    yklfsptllfllsrgkaatenwsienlfeekqndtadillvkdqfyelldvktrnisksa
    fapniisayklaqtcakmidnkefdlfdinylevdwelngedlvcvstsfaelfksepse
    lyinwaaamqiqfhvrdldqgfngtreewaksylkhfvtqaeqraismidkfvkpfkkyi
    

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.