PDB entry 3dl6

View 3dl6 on RCSB PDB site
Description: crystal structure of the a287f/s290g active site mutant of ts-dhfr from cryptosporidium hominis
Deposited on 2008-06-26, released 2008-08-19
The last revision was dated 2021-10-20, with a file datestamp of 2021-10-15.
Experiment type: XRAY
Resolution: 3.25 Å
R-factor: 0.225
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Dihydrofolate reductase, DHFR
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-520)
      • engineered mutation (286)
      • engineered mutation (289)
  • Chain 'B':
    Compound: Dihydrofolate reductase, DHFR
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-520)
      • engineered mutation (286)
      • engineered mutation (289)
  • Chain 'C':
    Compound: Dihydrofolate reductase, DHFR
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-520)
      • engineered mutation (286)
      • engineered mutation (289)
  • Chain 'D':
    Compound: Dihydrofolate reductase, DHFR
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-520)
      • engineered mutation (286)
      • engineered mutation (289)
  • Chain 'E':
    Compound: Dihydrofolate reductase, DHFR
    Species: Cryptosporidium hominis [TaxId:237895]
    Gene: Chro.40506
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5CGA3 (0-520)
      • engineered mutation (286)
      • engineered mutation (289)
  • Heterogens: UMP, CB3, DHF, NDP, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3dl6A (A:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records):
    >3dl6A (A:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqlksi
ddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengayren
rtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfikgdtngnhliekkvyiw
sgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklietlk
nnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspfnia
syailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkveni
edfkwedieligyypyptikmdmav

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3dl6B (B:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records):
    >3dl6B (B:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekql
ksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengay
renrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfikgdtngnhliekkv
yiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklie
tlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspf
niasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkv
eniedfkwedieligyypyptikmdmav

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3dl6C (C:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records):
    >3dl6C (C:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekql
ksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengay
renrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfikgdtngnhliekkv
yiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklie
tlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspf
niasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkv
eniedfkwedieligyypyptikmdmav

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3dl6D (D:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records):
    >3dl6D (D:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqekql
ksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengay
renrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfikgdtngnhliekkv
yiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlaklie
tlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspf
niasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkv
eniedfkwedieligyypyptikmdmav

  • Chain 'E':
    Sequence, based on SEQRES records:
    >3dl6E (E:)
mseknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwd
sigrrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyr
dalkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqek
ktlqncdpargqlksiddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqy
ldllsrvlengayrenrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfik
gdtngnhliekkvyiwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddy
tgvgvdqlaklietlknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnl
yqrscdlglgspfniasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrt
prpfpqlkfkrkveniedfkwedieligyypyptikmdmav

    Sequence, based on observed residues (ATOM records):
    >3dl6E (E:)
eknvsivvaasvlssgigingqlpwsisedlkffskitnnkcdsnkknalimgrktwdsi
grrplknriivvissslpqdeadpnvvvfrnledsienlmnddsienifvcggesiyrda
lkdnfvdriyltrvalediefdtyfpeipetflpvymsqtfctknisydfmifekqeklk
siddtvdllgeifgirkmgnrhkfpkeeiyntpsirfgrehyefqyldllsrvlengayr
enrtgistysifgqmmrfdmresfpllttkkvfirgifeeliwfikgdtngnhliekkvy
iwsgngskeyleriglghreendlgpiygfqwrhyngeyktmhddytgvgvdqlakliet
lknnpkdrrhiltawnpsalsqmalppchvlsqyyvtndnclscnlyqrscdlglgspfn
iasyailtmmlaqvcgyepgelaifigdahiyenhltqlkeqlsrtprpfpqlkfkrkve
niedfkwedieligyypyptikmdmav