PDB entry 3df2

View 3df2 on RCSB PDB site
Description: Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. This file contains the 50S subunit of the first 70S ribosome. The entire crystal structure contains two 70S ribosomes.
Class: ribosome
Keywords: RNA-protein complex, RIBOSOME
Deposited on 2008-06-11, released 2008-08-19
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-12-10, with a file datestamp of 2014-12-05.
Experiment type: XRAY
Resolution: 3.5 Å
R-factor: 0.269
AEROSPACI score: 0.03 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 50S ribosomal protein L32
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df201
  • Chain '1':
    Compound: 50S ribosomal protein L33
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df211
  • Chain '2':
    Compound: 50S ribosomal protein L34
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
  • Chain '3':
    Compound: 50S ribosomal protein L35
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df231
  • Chain '4':
    Compound: 50S ribosomal protein L36
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df241
  • Chain 'A':
    Compound: 5S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'B':
    Compound: 23S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'C':
    Compound: 50S ribosomal protein L2
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2c1, d3df2c2
  • Chain 'D':
    Compound: 50S ribosomal protein L3
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2d1
  • Chain 'E':
    Compound: 50S ribosomal protein L4
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2e1
  • Chain 'F':
    Compound: 50S ribosomal protein L5
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2f1
  • Chain 'G':
    Compound: 50S ribosomal protein L6
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2g1, d3df2g2
  • Chain 'H':
    Compound: 50S ribosomal protein L9
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2h1, d3df2h2
  • Chain 'I':
    Compound: 50S ribosomal protein L11
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2i1, d3df2i2
  • Chain 'J':
    Compound: 50S ribosomal protein L13
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2j1
  • Chain 'K':
    Compound: 50S ribosomal protein L14
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2k1
  • Chain 'L':
    Compound: 50S ribosomal protein L15
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2l1
  • Chain 'M':
    Compound: 50S ribosomal protein L16
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2m1
  • Chain 'N':
    Compound: 50S ribosomal protein L17
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2n1
  • Chain 'O':
    Compound: 50S ribosomal protein L18
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2o1
  • Chain 'P':
    Compound: 50S ribosomal protein L19
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2p1
  • Chain 'Q':
    Compound: 50S ribosomal protein L20
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2q1
  • Chain 'R':
    Compound: 50S ribosomal protein L21
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2r1
  • Chain 'S':
    Compound: 50S ribosomal protein L22
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2s1
  • Chain 'T':
    Compound: 50S ribosomal protein L23
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2t1
  • Chain 'U':
    Compound: 50S ribosomal protein L24
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2u1
  • Chain 'V':
    Compound: 50S ribosomal protein L25
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2v1
  • Chain 'W':
    Compound: 50S ribosomal protein L27
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2w1
  • Chain 'X':
    Compound: 50S ribosomal protein L29
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2x1
  • Chain 'Y':
    Compound: 50S ribosomal protein L30
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2y1
  • Chain 'Z':
    Compound: 50S ribosomal protein L28
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d3df2z1
  • Heterogens: MG, ZN, HOH

PDB Chain Sequences:

  • Chain '0':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df20 (0:)
avqqnkptrskrgmrrshdaltavtslsvdktsgekhlrhhitadgyyrgrkviak

  • Chain '1':
    Sequence, based on SEQRES records: (download) 
    >3df21 (1:)
akgirekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeakik

    Sequence, based on observed residues (ATOM records): (download) 
    >3df21 (1:)
girekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeak

  • Chain '2':
    No sequence available.

  • Chain '3':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df23 (3:)
pkiktvrgaakrfkktgkggfkhkhanlrhiltkkatkrkrhlrpkamvskgdlglviac
lpya

  • Chain '4':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df24 (4:)
mkvrasvkklcrnckivkrdgvirvicsaepkhkqrqg

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3df2C (C:)
mavvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkq
ayrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgv
daaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsge
mrkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnf
gkhpvtpwgvqtkgkktrsnkrtdkfivrrrsk

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2C (C:)
avvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkqa
yrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgvd
aaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsgem
rkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnfg
khpvtpwgvqtkgkktrsnkrtdkfivrrrs

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2D (D:)
miglvgkkvgmtriftedgvsipvtvieveanrvtqvkdlandgyraiqvttgakkanrv
tkpeaghfakagveagrglwefrlaegeeftvgqsisvelfadvkkvdvtgtskgkgfag
tvkrwnfrtqdathgnslshrvpgsigqnqtpgkvfkgkkmagqmgnervtvqsldvvrv
daernlllvkgavpgatgsdlivkpavka

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2E (E:)
melvlkdaqsaltvsettfgrdfnealvhqvvvayaagarqgtraqktraevtgsgkkpw
rqkgtgrarsgsikspiwrsggvtfaarpqdhsqkvnkkmyrgalksilselvrqdrliv
vekfsveapktkllaqklkdmaledvliitgeldenlflaarnlhkvdvrdatgidpvsl
iafdkvvmtadavkqveemla

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2F (F:)
aklhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaais
gqkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsaks
fdgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2G (G:)
srvakapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyadg
waqagtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpagi
taecptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtkeakkk

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2H (H:)
mqvilldkvanlgslgdqvnvkagyarnflvpqgkavpatkknieffearraeleaklae
vlaaanaraekinaletvtiaskagdegklfgsigtrdiadavtaagvevaksevrlpng
vlrttgehevsfqvhsevfakvivnvvae

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2I (I:)
akkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvitv
yadrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtgad
ieamtrsiegtarsmglvved

  • Chain 'J':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2J (J:)
mktftakpetvkrdwyvvdatgktlgrlatelarrlrgkhkaeytphvdtgdyiivlnad
kvavtgnkrtdkvyyhhtghiggikqatfeemiarrpervieiavkgmlpkgplgramfr
klkvyagnehnhaaqqpqvldi

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >3df2K (K:)
miqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlka
vvvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislap
evl

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2K (K:)
iqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlkav
vvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislape
v

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >3df2L (L:)
mrlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrr
lpkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpv
tvrglrvtkgaraaieaaggkiee

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2L (L:)
rlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrrl
pkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpvt
vrglrvtkgaraaieaaggkiee

  • Chain 'M':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2M (M:)
mlqpkrtkfrkmhkgrnrglaqgtdvsfgsfglkavgrgrltarqieaarramtravkrq
gkiwirvfpdkpitekplavrmgkgkgnveywvaliqpgkvlyemdgvpeelareafkla
aaklpikttfvtktvm

  • Chain 'N':
    Sequence, based on SEQRES records: (download) 
    >3df2N (N:)
mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse
kaeaaae

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2N (N:)
mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse

  • Chain 'O':
    Sequence, based on SEQRES records: (download) 
    >3df2O (O:)
mdkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiae
qlkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2O (O:)
dkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiaeq
lkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf

  • Chain 'P':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2P (P:)
sniikqleqeqmkqdvpsfrpgdtvevkvwvvegskkrlqafegvviairnrglhsaftv
rkisngegvervfqthspvvdsisvkrrgavrkaklyylrertgkaarikerln

  • Chain 'Q':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2Q (Q:)
arvkrgviararhkkilkqakgyygarsrvyrvafqavikagqyayrdrrqrkrqfrqlw
iarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftalvekakaala

  • Chain 'R':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2R (R:)
myavfqsggkqhrvsegqtvrlekldiatgetvefaevlmiangeevkigvpfvdggvik
aevvahgrgekvkivkfrrrkhyrkqqghrqwftdvkitgisa

  • Chain 'S':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2S (S:)
metiakhrharssaqkvrlvadlirgkkvsqaldiltytnkkaavlvkkvlesaianaeh
ndgadiddlkvtkifvdegpsmkrimprakgradrilkrtshitvvvsdr

  • Chain 'T':
    Sequence, based on SEQRES records: (download) 
    >3df2T (T:)
mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
lvvkgkvkrhgqrigrrsdwkkayvtlkegqnldfvggae

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2T (T:)
mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
lvvkgkvkrhgqrigrrsdwkkayvtlkegqnl

  • Chain 'U':
    Sequence, based on SEQRES records: (download) 
    >3df2U (U:)
aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnsetik

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2U (U:)
aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnseti

  • Chain 'V':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2V (V:)
mftinaevrkeqgkgasrrlraankfpaiiyggkeaplaieldhdkvmnmqakaefysev
ltivvdgkeikvkaqdvqrhpykpklqhidfvra

  • Chain 'W':
    Sequence, based on SEQRES records: (download) 
    >3df2W (W:)
ahkkaggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfa
kadgkvkfevkgpknrkfisieae

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2W (W:)
ggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfakadgk
vkfevkgpknrkfisieae

  • Chain 'X':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2X (X:)
mkakelreksveelntellnllreqfnlrmqaasgqlqqshllkqvrrdvarvktllnek
aga

  • Chain 'Y':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3df2Y (Y:)
aktikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee

  • Chain 'Z':
    Sequence, based on SEQRES records: (download) 
    >3df2Z (Z:)
msrvcqvtgkrpvtgnnrshalnatkrrflpnlhshrfwvesekrfvtlrvsakgmrvid
kkgidtvlaelrargeky

    Sequence, based on observed residues (ATOM records): (download) 
    >3df2Z (Z:)
srvcqvtgkrpvtgnnrshalnatkrrflpnlhshrfwvesekrfvtlrvsakgmrvidk
kgidtvlaelrargeky