PDB entry 3d2b

View 3d2b on RCSB PDB site
Description: Structure of 2D9, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution
Class: hydrolase
Keywords: lipase, alpha/beta hydrolase, stability, directed evolution, Hydrolase, Lipid degradation, Secreted
Deposited on 2008-05-08, released 2008-06-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lipase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: LIPA, LIP, BSU02700
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37957 (Start-180)
      • engineered (16)
      • engineered (88)
      • engineered (113)
      • engineered (131)
      • engineered (156)
      • engineered (165)
    Domains in SCOPe 2.08: d3d2ba_
  • Chain 'B':
    Compound: Lipase
    Species: Bacillus subtilis [TaxId:1423]
    Gene: LIPA, LIP, BSU02700
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37957 (Start-180)
      • engineered (16)
      • engineered (88)
      • engineered (113)
      • engineered (131)
      • engineered (156)
      • engineered (165)
    Domains in SCOPe 2.08: d3d2bb_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3d2bA (A:)
aehnpvvmvhgiggassnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvq
kvldetgakkvdivahsmggantlyyikyldggnkvanvvtlgganrlttgkappgtdpn
qkilytsiyssddmivmnylsrldgarnvqihgvghmgllyssqvyslikeglngggqnt
n

    Sequence, based on observed residues (ATOM records): (download) 
    >3d2bA (A:)
hnpvvmvhgiggassnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvqkv
ldetgakkvdivahsmggantlyyikyldggnkvanvvtlgganrlttgkappgtdpnqk
ilytsiyssddmivmnylsrldgarnvqihgvghmgllyssqvyslikeglngggqntn

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3d2bB (B:)
aehnpvvmvhgiggassnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvq
kvldetgakkvdivahsmggantlyyikyldggnkvanvvtlgganrlttgkappgtdpn
qkilytsiyssddmivmnylsrldgarnvqihgvghmgllyssqvyslikeglngggqnt
n

    Sequence, based on observed residues (ATOM records): (download) 
    >3d2bB (B:)
hnpvvmvhgiggassnfagiksylvsqgwsrdklyavdfwdktgtnynngpvlsrfvqkv
ldetgakkvdivahsmggantlyyikyldggnkvanvvtlgganrlttgkappgtdpnqk
ilytsiyssddmivmnylsrldgarnvqihgvghmgllyssqvyslikeglngggqntn