PDB entry 3d0a

View 3d0a on RCSB PDB site
Description: Human p53 core domain with hot spot mutation R249S and second site suppressor mutation H168R in sequence-specific complex with DNA
Class: transcription/DNA
Keywords: p53, Mutant protein, Loop-sheet-helix motif, DNA target, Acetylation, Activator, Alternative splicing, Anti-oncogene, Apoptosis, Cell cycle, Covalent protein-RNA linkage, Cytoplasm, Disease mutation, DNA-binding, Endoplasmic reticulum, Glycoprotein, Host-virus interaction, Li-Fraumeni syndrome, Metal-binding, Methylation, Nucleus, Phosphoprotein, Polymorphism, Transcription, Transcription regulation, Ubl conjugation, Zinc, TRANSCRIPTION/DNA COMPLEX
Deposited on 2008-05-01, released 2009-01-20
The last revision prior to the SCOPe 2.03 freeze date was dated 2009-01-20, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.186
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Cellular tumor antigen p53
    Species: Homo sapiens [TaxId:9606]
    Gene: TP53
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04637
      • engineered (74)
      • engineered (155)
    Domains in SCOPe 2.03: d3d0aa_
  • Chain 'B':
    Compound: Cellular tumor antigen p53
    Species: Homo sapiens [TaxId:9606]
    Gene: TP53
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04637
      • engineered (74)
      • engineered (155)
    Domains in SCOPe 2.03: d3d0ab_
  • Chain 'C':
    Compound: Cellular tumor antigen p53
    Species: Homo sapiens [TaxId:9606]
    Gene: TP53
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04637
      • engineered (74)
      • engineered (155)
    Domains in SCOPe 2.03: d3d0ac_
  • Chain 'D':
    Compound: Cellular tumor antigen p53
    Species: Homo sapiens [TaxId:9606]
    Gene: TP53
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04637
      • engineered (74)
      • engineered (155)
    Domains in SCOPe 2.03: d3d0ad_
  • Chain 'E':
    Compound: DNA (5'-d(*dcp*dgp*dgp*dgp*dcp*dap*dtp*dgp*dcp*dcp*dcp*dg)-3')
    Species: synthetic, synthetic
  • Chain 'F':
    Compound: DNA (5'-d(*dcp*dgp*dgp*dgp*dcp*dap*dtp*dgp*dcp*dcp*dcp*dg)-3')
    Species: synthetic, synthetic
  • Chain 'G':
    Compound: DNA (5'-d(*dcp*dgp*dgp*dgp*dcp*dap*dtp*dgp*dcp*dcp*dcp*dg)-3')
    Species: synthetic, synthetic
  • Chain 'H':
    Compound: DNA (5'-d(*dcp*dgp*dgp*dgp*dcp*dap*dtp*dgp*dcp*dcp*dcp*dg)-3')
    Species: synthetic, synthetic
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3d0aA (A:)
sssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstppp
gtrvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfr
hsvvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevr
vcacpgrdrrteeenlrkkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3d0aA (A:)
svpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstpppgt
rvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfrhs
vvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevrvc
acpgrdrrteeenlrk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3d0aB (B:)
sssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstppp
gtrvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfr
hsvvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevr
vcacpgrdrrteeenlrkkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3d0aB (B:)
ssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstpppg
trvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfrh
svvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevrv
cacpgrdrrteeenlrk

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3d0aC (C:)
sssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstppp
gtrvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfr
hsvvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevr
vcacpgrdrrteeenlrkkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3d0aC (C:)
svpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstpppgt
rvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfrhs
vvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevrvc
acpgrdrrteeen

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3d0aD (D:)
sssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstppp
gtrvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfr
hsvvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevr
vcacpgrdrrteeenlrkkg

    Sequence, based on observed residues (ATOM records): (download) 
    >3d0aD (D:)
ssvpsqktyqgsygfrlgflhsgtaksvtctyspalnkmfcqlaktcpvqlwvdstpppg
trvramaiykqsqrmtevvrrcphhercsdsdglappqhlirvegnlrveylddrntfrh
svvvpyeppevgsdcttihynymcnsscmggmnrspiltiitledssgnllgrnsfevrv
cacpgrdrrteeen

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.