PDB entry 3czo

View 3czo on RCSB PDB site
Description: crystal structure of double mutant phenylalanine ammonia-lyase from anabaena variabilis
Deposited on 2008-04-29, released 2008-05-20
The last revision was dated 2021-10-20, with a file datestamp of 2021-10-15.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.214
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: histidine ammonia-lyase
    Species: Anabaena variabilis [TaxId:1172]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3M5Z3 (0-540)
      • engineered mutation (478)
      • engineered mutation (540)
  • Chain 'B':
    Compound: histidine ammonia-lyase
    Species: Anabaena variabilis [TaxId:1172]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3M5Z3 (0-540)
      • engineered mutation (478)
      • engineered mutation (540)
  • Chain 'C':
    Compound: histidine ammonia-lyase
    Species: Anabaena variabilis [TaxId:1172]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3M5Z3 (0-540)
      • engineered mutation (478)
      • engineered mutation (540)
  • Chain 'D':
    Compound: histidine ammonia-lyase
    Species: Anabaena variabilis [TaxId:1172]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q3M5Z3 (0-540)
      • engineered mutation (478)
      • engineered mutation (540)
  • Heterogens: HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3czoA (A:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdilp
s

    Sequence, based on observed residues (ATOM records):
    >3czoA (A:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdil

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records:
    >3czoB (B:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdilp
s

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3czoC (C:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdilp
s

    Sequence, based on observed residues (ATOM records):
    >3czoC (C:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdil

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3czoD (D:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrdheliqdryslrclpqylg
pivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllak
hldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfp
thaeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasl
spaterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdilp
s

    Sequence, based on observed residues (ATOM records):
    >3czoD (D:)
nviignqkltindvarvarngtlvsltnntdilqgiqascdyinnavesgepiygvtsgf
ggmanvaisreqaselqtnlvwflktgagnklpladvraamllranshmrgasgirleli
krmeiflnagvtpyvyefgsigasgdlvplsyitgsligldpsfkvdfngkemdaptalr
qlnlspltllpkeglammngtsvmtgiaancvydtqiltaiamgvhaldiqalngtnqsf
hpfihnskphpgqlwaadqmisllansqlvrdeldgkhdyrheliqdryslrclpqylgp
ivdgisqiakqieieinsvtdnplidvdnqasyhggnflgqyvgmgmdhlryyigllakh
ldvqiallaspefsnglppsllgnrerkvnmglkglqicgnsimplltfygnsiadrfpt
haeqfnqninsqgytsatlarrsvdifqnyvaialmfgvqavdlrtykktghydarasls
paterlysavrhvvgqkptsdrpyiwndneqgldehiarisadiaaggvivqavqdilp