PDB entry 3bud

View 3bud on RCSB PDB site
Description: Golgi mannosidase II D204A catalytic nucleophile mutant with an empty active site
Class: hydrolase
Keywords: GLYCOSYL HYDROLASE FAMILY 38, Glycosidase, Golgi apparatus, Membrane, Signal-anchor, Transmembrane, HYDROLASE
Deposited on 2008-01-02, released 2008-07-01
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: N/A
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Alpha-mannosidase 2
    Species: Drosophila melanogaster
    Gene: alpha-Man-II, GmII
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q24451 (Start-1044)
      • engineered (203)
      • see remark 999 (906)
    Domains in SCOPe 2.08: d3buda1, d3buda2, d3buda3
  • Heterogens: NAG, PO4, ZN, MPD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3budA (A:)
rsshhhhhhgefddpirpplkvarsprpgqcqdvvqdvpnvdvqmlelydrmsfkdidgg
vwkqgwnikydplkynahhklkvfvvphshndpgwiqtfeeyyqhdtkhilsnalrhlhd
npemkfiwaeisyfarfyhdlgenkklqmksivkngqlefvtggwvmpdeanshwrnvll
qltegqtwlkqfmnvtptaswaiapfghsptmpyilqksgfknmliqrthysvkkelaqq
rqleflwrqiwdnkgdtalfthmmpfysydiphtcgpdpkvccqfdfkrmgsfglscpwk
vpprtisdqnvaarsdllvdqwkkkaelyrtnvlliplgddfrfkqntewdvqrvnyerl
fehinsqahfnvqaqfgtlqeyfdavhqaeragqaefptlsgdfftyadrsdnywsgyyt
srpyhkrmdrvlmhyvraaemlsawhswdgmarieerleqarrelslfqhhdgitgtakt
hvvvdyeqrmqealkacqmvmqqsvyrlltkpsiyspdfsfsyftlddsrwpgsgvedsr
ttiilgedilpskhvvmhntlphwreqlvdfyvsspfvsvtdlannpveaqvspvwswhh
dtltktihpqgsttkyriifkarvppmglatyvltisdskpehtsyasnlllrknptslp
lgqypedvkfgdpreislrvgngptlafseqgllksiqltqdsphvpvhfkflkygvrsh
gdrsgaylflpngpaspvelgqpvvlvtkgklessvsvglpsvvhqtimrggapeirnlv
digsldnteivmrlethidsgdifytdlnglqfikrrrldklplqanyypipsgmfieda
ntrltlltgqplggsslasgeleimqdrrlasdderglgqgvldnkpvlhiyrlvlekvn
ncvrpsklhpagyltsaahkasqslldpldkfifaenewigaqgqfggdhpsaredldvs
vmrrltkssaktqrvgyvlhrtnlmqcgtpeehtqkldvchllpnvarcerttltflqnl
ehldgmvapevcpmetaayvsshss

    Sequence, based on observed residues (ATOM records): (download) 
    >3budA (A:)
qcqdvvqdvpnvdvqmlelydrmsfkdidggvwkqgwnikydplkynahhklkvfvvphs
hndpgwiqtfeeyyqhdtkhilsnalrhlhdnpemkfiwaeisyfarfyhdlgenkklqm
ksivkngqlefvtggwvmpdeanshwrnvllqltegqtwlkqfmnvtptaswaiapfghs
ptmpyilqksgfknmliqrthysvkkelaqqrqleflwrqiwdnkgdtalfthmmpfysy
diphtcgpdpkvccqfdfkrmgsfglscpwkvpprtisdqnvaarsdllvdqwkkkaely
rtnvlliplgddfrfkqntewdvqrvnyerlfehinsqahfnvqaqfgtlqeyfdavhqa
eragqaefptlsgdfftyadrsdnywsgyytsrpyhkrmdrvlmhyvraaemlsawhswd
gmarieerleqarrelslfqhhdgitgtakthvvvdyeqrmqealkacqmvmqqsvyrll
tkpsiyspdfsfsyftlddsrwpgsgvedsrttiilgedilpskhvvmhntlphwreqlv
dfyvsspfvsvtdlannpveaqvspvwswhhdtltktihpqgsttkyriifkarvppmgl
atyvltisdskpehtsyasnlllrknptslplgqypedvkfgdpreislrvgngptlafs
eqgllksiqltqdsphvpvhfkflkygvrshgdrsgaylflpngpaspvelgqpvvlvtk
gklessvsvglpsvvhqtimrggapeirnlvdigsldnteivmrlethidsgdifytdln
glqfikrrrldklplqanyypipsgmfiedantrltlltgqplggsslasgeleimqdrr
lasdderglgqgvldnkpvlhiyrlvlekvnncvrpsklhpagyltsaahkasqslldpl
dkfifaenewigaqgqfggdhpsaredldvsvmrrltkssaktqrvgyvlhrtnlmqcgt
peehtqkldvchllpnvarcerttltflqnlehldgmvapevcpmetaayvsshss