PDB entry 3b8u

View 3b8u on RCSB PDB site
Description: crystal structure of escherichia coli alaine racemase mutant e221a
Deposited on 2007-11-02, released 2008-07-08
The last revision was dated 2021-11-10, with a file datestamp of 2021-11-05.
Experiment type: XRAY
Resolution: 3 Å
R-factor: 0.215
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alanine racemase
    Species: Escherichia coli [TaxId:83333]
    Gene: alr
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6B4 (20-378)
      • expression tag (0-19)
      • engineered mutation (240)
  • Chain 'B':
    Compound: alanine racemase
    Species: Escherichia coli [TaxId:83333]
    Gene: alr
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6B4 (20-378)
      • expression tag (0-19)
      • engineered mutation (240)
  • Chain 'C':
    Compound: alanine racemase
    Species: Escherichia coli [TaxId:83333]
    Gene: alr
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6B4 (20-378)
      • expression tag (0-19)
      • engineered mutation (240)
  • Chain 'D':
    Compound: alanine racemase
    Species: Escherichia coli [TaxId:83333]
    Gene: alr
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6B4 (20-378)
      • expression tag (0-19)
      • engineered mutation (240)
  • Heterogens: SO4, PLP, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3b8uA (A:)
mgsshhhhhhssglvprgshmqaatvvinrralrhnlqrlrelapaskmvavvkanaygh
glletartlpdadafgvarleealrlraggitkpvlllegffdardlptisaqhfhtavh
neeqlaaleeasldepvtvwmkldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshf
aradepkcgatekqlaifntfcegkpgqrsiaasggillwpqshfdwvrpgiilygvspl
adrstgadfgcqpvmsltssliavrehkagepvgyggtwvserdtrlgvvamgygdgypr
aapsgtpvlvngrevpivgrvamdmicvdlgpqaqdkagdpvilwgeglpveriaemtkv
sayelitrltsrvamkyvd

    Sequence, based on observed residues (ATOM records):
    >3b8uA (A:)
mqaatvvinrralrhnlqrlrelapaskmvavvkanayghglletartlpdadafgvarl
eealrlraggitkpvlllegffdardlptisaqhfhtavhneeqlaaleeasldepvtvw
mkldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshfaradepkcgatekqlaifnt
fcegkpgqrsiaasggillwpqshfdwvrpgiilygvspladrstgadfgcqpvmsltss
liavrehkagepvgyggtwvserdtrlgvvamgygdgypraapsgtpvlvngrevpivgr
vamdmicvdlgpqaqdkagdpvilwgeglpveriaemtkvsayelitrltsrvamkyvd

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3b8uB (B:)
mgsshhhhhhssglvprgshmqaatvvinrralrhnlqrlrelapaskmvavvkanaygh
glletartlpdadafgvarleealrlraggitkpvlllegffdardlptisaqhfhtavh
neeqlaaleeasldepvtvwmkldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshf
aradepkcgatekqlaifntfcegkpgqrsiaasggillwpqshfdwvrpgiilygvspl
adrstgadfgcqpvmsltssliavrehkagepvgyggtwvserdtrlgvvamgygdgypr
aapsgtpvlvngrevpivgrvamdmicvdlgpqaqdkagdpvilwgeglpveriaemtkv
sayelitrltsrvamkyvd

    Sequence, based on observed residues (ATOM records):
    >3b8uB (B:)
qaatvvinrralrhnlqrlrelapaskmvavvkanayghglletartlpdadafgvarle
ealrlraggitkpvlllegffdardlptisaqhfhtavhneeqlaaleeasldepvtvwm
kldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshfaradepkcgatekqlaifntf
cegkpgqrsiaasggillwpqshfdwvrpgiilygvspladrstgadfgcqpvmsltssl
iavrehkagepvgyggtwvserdtrlgvvamgygdgypraapsgtpvlvngrevpivgrv
amdmicvdlgpqaqdkagdpvilwgeglpveriaemtkvsayelitrltsrvamkyvd

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3b8uC (C:)
mgsshhhhhhssglvprgshmqaatvvinrralrhnlqrlrelapaskmvavvkanaygh
glletartlpdadafgvarleealrlraggitkpvlllegffdardlptisaqhfhtavh
neeqlaaleeasldepvtvwmkldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshf
aradepkcgatekqlaifntfcegkpgqrsiaasggillwpqshfdwvrpgiilygvspl
adrstgadfgcqpvmsltssliavrehkagepvgyggtwvserdtrlgvvamgygdgypr
aapsgtpvlvngrevpivgrvamdmicvdlgpqaqdkagdpvilwgeglpveriaemtkv
sayelitrltsrvamkyvd

    Sequence, based on observed residues (ATOM records):
    >3b8uC (C:)
aatvvinrralrhnlqrlrelapaskmvavvkanayghglletartlpdadafgvarlee
alrlraggitkpvlllegffdardlptisaqhfhtavhneeqlaaleeasldepvtvwmk
ldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshfaradepkcgatekqlaifntfc
egkpgqrsiaasggillwpqshfdwvrpgiilygvspladrstgadfgcqpvmsltssli
avrehkagepvgyggtwvserdtrlgvvamgygdgypraapsgtpvlvngrevpivgrva
mdmicvdlgpqaqdkagdpvilwlpverikvsayelitrltsrvamkyvd

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3b8uD (D:)
mgsshhhhhhssglvprgshmqaatvvinrralrhnlqrlrelapaskmvavvkanaygh
glletartlpdadafgvarleealrlraggitkpvlllegffdardlptisaqhfhtavh
neeqlaaleeasldepvtvwmkldtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshf
aradepkcgatekqlaifntfcegkpgqrsiaasggillwpqshfdwvrpgiilygvspl
adrstgadfgcqpvmsltssliavrehkagepvgyggtwvserdtrlgvvamgygdgypr
aapsgtpvlvngrevpivgrvamdmicvdlgpqaqdkagdpvilwgeglpveriaemtkv
sayelitrltsrvamkyvd

    Sequence, based on observed residues (ATOM records):
    >3b8uD (D:)
aatvvinrralrhnlqrlrelapaskmvavvkanayghglletartlpdadafgvarlee
alrlraggitkpvlllegffdardlptisaqhfhtavhneeqlaaleealdepvtvwmkl
dtgmhrlgvrpeqaeafyhrltqcknvrqpvnivshfaradepkcgatekqlaifntfce
gkpgqrsiaasggillwpqshfdwvrpgiilygvsplstgadfgcqpvmsltssliavre
hkagepvgyggtwvserdtrlgvvamgygdgypraapsgtpvlvngrevpivgrvamdmi
cvdlgpqaqdkagdpvilwgeglpverikvsayelitrltsrvamkyvd