PDB entry 3b82

View 3b82 on RCSB PDB site
Description: Structure of the eEF2-ExoA(E546H)-NAD+ complex
Class: biosynthetic protein/transferase
Keywords: elongation factor, toxin, ADP-ribosylation, toxin-substrate complex, Cytoplasm, GTP-binding, Nucleotide-binding, Phosphorylation, Protein biosynthesis, RNA-binding, rRNA-binding, Glycosyltransferase, NAD, Transferase, BIOSYNTHETIC PROTEIN/TRANSFERASE COMPLEX
Deposited on 2007-10-31, released 2008-06-17
The last revision prior to the SCOPe 2.07 freeze date was dated 2009-05-05, with a file datestamp of 2009-05-01.
Experiment type: XRAY
Resolution: 2.35 Å
R-factor: 0.215
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Elongation factor 2
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3b82a1, d3b82a2, d3b82a3, d3b82a4, d3b82a5
  • Chain 'B':
    Compound: exotoxin a
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: eta
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11439 (1-206)
      • expression tag (0)
      • see remark 999 (8)
      • see remark 999 (116)
      • engineered (147)
    Domains in SCOPe 2.07: d3b82b1, d3b82b2
  • Chain 'C':
    Compound: Elongation factor 2
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3b82c1, d3b82c2, d3b82c3, d3b82c4, d3b82c5
  • Chain 'D':
    Compound: exotoxin a
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: eta
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11439 (1-206)
      • expression tag (0)
      • see remark 999 (8)
      • see remark 999 (116)
      • engineered (147)
    Domains in SCOPe 2.07: d3b82d1, d3b82d2
  • Chain 'E':
    Compound: Elongation factor 2
    Species: Saccharomyces cerevisiae [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d3b82e1, d3b82e2, d3b82e3, d3b82e4, d3b82e5
  • Chain 'F':
    Compound: exotoxin a
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: eta
    Database cross-references and differences (RAF-indexed):
    • Uniprot P11439 (1-206)
      • expression tag (0)
      • see remark 999 (8)
      • see remark 999 (116)
      • engineered (147)
    Domains in SCOPe 2.07: d3b82f1, d3b82f2
  • Heterogens: NAD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3b82A (A:)
    mvaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisaakagearftdtr
    kdeqergitikstaislysemsdedvkeikqktdgnsflinlidspghvdfssevtaalr
    vtdgalvvvdtiegvcvqtetvlrqalgerikpvvvinkvdrallelqvskedlyqtfar
    tvesvnvivstyadevlgdvqvypargtvafgsglhgwaftirqfatryakkfgvdkakm
    mdrlwgdsffnpktkkwtnkdtdaegkplerafnmfildpifrlftaimnfkkdeipvll
    ekleivlkgdekdlegkallkvvmrkflpaadallemivlhlpspvtaqayraeqlyegp
    addanciaikncdpkadlmlyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvp
    gkkddlfikaiqrvvlmmgrfvepiddcpagniiglvgidqfllktgtlttsetahnmkv
    mkfsvspvvqvavevknandlpklveglkrlsksdpcvltymsesgehivagtgelhlei
    clqdlehdhagvplkisppvvayretvesessqtalskspnkhnriylkaepideevsla
    iengiinprddfkararimaddygwdvtdarkiwcfgpdgngpnlvidqtkavqylheik
    dsvvaafqwatkegpifgeemrsvrvnildvtlhadaihrgggqiiptmrratyagflla
    dpkiqepvflveiqcpeqavggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgf
    tgelrqatggqafpqmvfdhwstlgsdpldptskageivlaarkrhgmkeevpgwqeyyd
    kl
    

    Sequence, based on observed residues (ATOM records): (download)
    >3b82A (A:)
    vaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisagitikstaislys
    emsdedvkeikqktdgnsflinlidspghvdfssevtaalrvtdgalvvvdtiegvcvqt
    etvlrqalgerikpvvvinkvdrallelqvskedlyqtfartvesvnvivstyadevlgd
    vqvypargtvafgsglhgwaftirqfatryakkfgvdkakmmdrlwgdsffnpktkkwtn
    kdtdaegkplerafnmfildpifrlftaimnfkkdeipvllekleivlkgdekdlegkal
    lkvvmrkflpaadallemivlhlpspvtaqayraeqlyegpaddanciaikncdpkadlm
    lyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvpgkkddlfikaiqrvvlmmg
    rfvepiddcpagniiglvgidqfllktgtlttsetahnmkvmkfsvspvvqvavevknan
    dlpklveglkrlsksdpcvltymsesgehivagtgelhleiclqdlehdhagvplkispp
    vvayretvesessqtalskspnkhnriylkaepideevslaiengiinprddfkararim
    addygwdvtdarkiwcfgpdgngpnlvidqtkavqylheikdsvvaafqwatkegpifge
    emrsvrvnildvtlhadaihrgggqiiptmrratyagflladpkiqepvflveiqcpeqa
    vggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgftgelrqatggqafpqmvfd
    hwstlgsdpldptskageivlaarkrhgmkeevpgwqeyydkl
    

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3b82B (B:)
    aflgdggdvsfstrgtqnwtverllqahrqleergyvfvgyhgtfleaaqsivfggvrar
    sqdldaiwrgfyiagdpalaygyaqdqepdargrirngallrvyvprsslpgfyrtsltl
    aapeaageverlighplplrldaitgpheeggrletilgwplaertvvipsaiptdprnv
    ggdldpssipdkeqaisalpdyasqpg
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3b82C (C:)
    mvaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisaakagearftdtr
    kdeqergitikstaislysemsdedvkeikqktdgnsflinlidspghvdfssevtaalr
    vtdgalvvvdtiegvcvqtetvlrqalgerikpvvvinkvdrallelqvskedlyqtfar
    tvesvnvivstyadevlgdvqvypargtvafgsglhgwaftirqfatryakkfgvdkakm
    mdrlwgdsffnpktkkwtnkdtdaegkplerafnmfildpifrlftaimnfkkdeipvll
    ekleivlkgdekdlegkallkvvmrkflpaadallemivlhlpspvtaqayraeqlyegp
    addanciaikncdpkadlmlyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvp
    gkkddlfikaiqrvvlmmgrfvepiddcpagniiglvgidqfllktgtlttsetahnmkv
    mkfsvspvvqvavevknandlpklveglkrlsksdpcvltymsesgehivagtgelhlei
    clqdlehdhagvplkisppvvayretvesessqtalskspnkhnriylkaepideevsla
    iengiinprddfkararimaddygwdvtdarkiwcfgpdgngpnlvidqtkavqylheik
    dsvvaafqwatkegpifgeemrsvrvnildvtlhadaihrgggqiiptmrratyagflla
    dpkiqepvflveiqcpeqavggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgf
    tgelrqatggqafpqmvfdhwstlgsdpldptskageivlaarkrhgmkeevpgwqeyyd
    kl
    

    Sequence, based on observed residues (ATOM records): (download)
    >3b82C (C:)
    vaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisagitikstaislys
    emsdedvkeikqktdgnsflinlidspghvdfssevtaalrvtdgalvvvdtiegvcvqt
    etvlrqalgerikpvvvinkvdrallelqvskedlyqtfartvesvnvivstyadevlgd
    vqvypargtvafgsglhgwaftirqfatryakkfgvdkakmmdrlwgdsffnpktkkwtn
    kdtdaegkplerafnmfildpifrlftaimnfkkdeipvllekleivlkgdekdlegkal
    lkvvmrkflpaadallemivlhlpspvtaqayraeqlyegpaddanciaikncdpkadlm
    lyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvpgkkddlfikaiqrvvlmmg
    rfvepiddcpagniiglvgidqfllktgtlttsetahnmkvmkfsvspvvqvavevknan
    dlpklveglkrlsksdpcvltymsesgehivagtgelhleiclqdlehdhagvplkispp
    vvayretvesessqtalskspnkhnriylkaepideevslaiengiinprddfkararim
    addygwdvtdarkiwcfgpdgngpnlvidqtkavqylheikdsvvaafqwatkegpifge
    emrsvrvnildvtlhadaihrgggqiiptmrratyagflladpkiqepvflveiqcpeqa
    vggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgftgelrqatggqafpqmvfd
    hwstlgsdpldptskageivlaarkrhgmkeevpgwqeyydkl
    

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3b82D (D:)
    aflgdggdvsfstrgtqnwtverllqahrqleergyvfvgyhgtfleaaqsivfggvrar
    sqdldaiwrgfyiagdpalaygyaqdqepdargrirngallrvyvprsslpgfyrtsltl
    aapeaageverlighplplrldaitgpheeggrletilgwplaertvvipsaiptdprnv
    ggdldpssipdkeqaisalpdyasqpg
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >3b82E (E:)
    mvaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisaakagearftdtr
    kdeqergitikstaislysemsdedvkeikqktdgnsflinlidspghvdfssevtaalr
    vtdgalvvvdtiegvcvqtetvlrqalgerikpvvvinkvdrallelqvskedlyqtfar
    tvesvnvivstyadevlgdvqvypargtvafgsglhgwaftirqfatryakkfgvdkakm
    mdrlwgdsffnpktkkwtnkdtdaegkplerafnmfildpifrlftaimnfkkdeipvll
    ekleivlkgdekdlegkallkvvmrkflpaadallemivlhlpspvtaqayraeqlyegp
    addanciaikncdpkadlmlyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvp
    gkkddlfikaiqrvvlmmgrfvepiddcpagniiglvgidqfllktgtlttsetahnmkv
    mkfsvspvvqvavevknandlpklveglkrlsksdpcvltymsesgehivagtgelhlei
    clqdlehdhagvplkisppvvayretvesessqtalskspnkhnriylkaepideevsla
    iengiinprddfkararimaddygwdvtdarkiwcfgpdgngpnlvidqtkavqylheik
    dsvvaafqwatkegpifgeemrsvrvnildvtlhadaihrgggqiiptmrratyagflla
    dpkiqepvflveiqcpeqavggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgf
    tgelrqatggqafpqmvfdhwstlgsdpldptskageivlaarkrhgmkeevpgwqeyyd
    kl
    

    Sequence, based on observed residues (ATOM records): (download)
    >3b82E (E:)
    vaftvdqmrslmdkvtnvrnmsviahvdhgkstltdslvqragiisagitikstaislys
    emsdedvkeikqktdgnsflinlidspghvdfssevtaalrvtdgalvvvdtiegvcvqt
    etvlrqalgerikpvvvinkvdrallelqvskedlyqtfartvesvnvivstyadevlgd
    vqvypargtvafgsglhgwaftirqfatryakkfgvdkakmmdrlwgdsffnpktkkwtn
    kdtdaegkplerafnmfildpifrlftaimnfkkdeipvllekleivlkgdekdlegkal
    lkvvmrkflpaadallemivlhlpspvtaqayraeqlyegpaddanciaikncdpkadlm
    lyvskmvptsdkgrfyafgrvfagtvksgqkvriqgpnyvpgkkddlfikaiqrvvlmmg
    rfvepiddcpagniiglvgidqfllktgtlttsetahnmkvmkfsvspvvqvavevknan
    dlpklveglkrlsksdpcvltymsesgehivagtgelhleiclqdlehdhagvplkispp
    vvayretvesessqtalskspnkhnriylkaepideevslaiengiinprddfkararim
    addygwdvtdarkiwcfgpdgngpnlvidqtkavqylheikdsvvaafqwatkegpifge
    emrsvrvnildvtlhadaihrgggqiiptmrratyagflladpkiqepvflveiqcpeqa
    vggiysvlnkkrgqvvseeqrpgtplftvkaylpvnesfgftgelrqatggqafpqmvfd
    hwstlgsdpldptskageivlaarkrhgmkeevpgwqeyydkl
    

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download)
    >3b82F (F:)
    aflgdggdvsfstrgtqnwtverllqahrqleergyvfvgyhgtfleaaqsivfggvrar
    sqdldaiwrgfyiagdpalaygyaqdqepdargrirngallrvyvprsslpgfyrtsltl
    aapeaageverlighplplrldaitgpheeggrletilgwplaertvvipsaiptdprnv
    ggdldpssipdkeqaisalpdyasqpg