PDB entry 3b12

View 3b12 on RCSB PDB site
Description: Crystal Structure of the Fluoroacetate Dehalogenase D104 mutant from Burkholderia sp. FA1 in complex with fluoroacetate
Class: hydrolase
Keywords: dehalogease, Fluoroacetate, HYDROLASE
Deposited on 2011-06-19, released 2012-06-20
The last revision prior to the SCOPe 2.06 freeze date was dated 2013-06-19, with a file datestamp of 2013-06-14.
Experiment type: XRAY
Resolution: 1.2 Å
R-factor: 0.177
AEROSPACI score: 0.81 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Fluoroacetate Dehalogenase
    Species: Burkholderia sp. [TaxId:36773]
    Gene: fac-dex
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1JU72 (0-End)
      • engineered mutation (103)
    Domains in SCOPe 2.06: d3b12a_
  • Chain 'B':
    Compound: Fluoroacetate Dehalogenase
    Species: Burkholderia sp. [TaxId:36773]
    Gene: fac-dex
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q1JU72 (0-End)
      • engineered mutation (103)
    Domains in SCOPe 2.06: d3b12b_
  • Heterogens: FAH, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3b12A (A:)
mfegferrlvdvgdvtincvvggsgpallllhgfpqnlhmwarvapllaneytvvcadlr
gyggsskpvgapdhanysframasdqrelmrtlgferfhlvgharggrtghrmaldhpds
vlslavldiiptyvmfeevdrfvaraywhwyflqqpapypekvigadpdtfyegclfgwg
atgadgfdpeqleeyrkqwrdpaaihgsccdyraggtidfeldhgdlgrqvqcpalvfsg
saglmhslfemqvvwaprlanmrfaslpgghffvdrfpddtarilreflsdarsgihqte
rres

    Sequence, based on observed residues (ATOM records): (download) 
    >3b12A (A:)
mfegferrlvdvgdvtincvvggsgpallllhgfpqnlhmwarvapllaneytvvcadlr
gyggsskpvgapdhanysframasdqrelmrtlgferfhlvgharggrtghrmaldhpds
vlslavldiiptyvmfeevdrfvaraywhwyflqqpapypekvigadpdtfyegclfgwg
atgadgfdpeqleeyrkqwrdpaaihgsccdyraggtidfeldhgdlgrqvqcpalvfsg
saglmhslfemqvvwaprlanmrfaslpgghffvdrfpddtarilreflsdars

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3b12B (B:)
mfegferrlvdvgdvtincvvggsgpallllhgfpqnlhmwarvapllaneytvvcadlr
gyggsskpvgapdhanysframasdqrelmrtlgferfhlvgharggrtghrmaldhpds
vlslavldiiptyvmfeevdrfvaraywhwyflqqpapypekvigadpdtfyegclfgwg
atgadgfdpeqleeyrkqwrdpaaihgsccdyraggtidfeldhgdlgrqvqcpalvfsg
saglmhslfemqvvwaprlanmrfaslpgghffvdrfpddtarilreflsdarsgihqte
rres

    Sequence, based on observed residues (ATOM records): (download) 
    >3b12B (B:)
mfegferrlvdvgdvtincvvggsgpallllhgfpqnlhmwarvapllaneytvvcadlr
gyggsskpvgapdhanysframasdqrelmrtlgferfhlvgharggrtghrmaldhpds
vlslavldiiptyvmfeevdrfvaraywhwyflqqpapypekvigadpdtfyegclfgwg
atgadgfdpeqleeyrkqwrdpaaihgsccdyraggtidfeldhgdlgrqvqcpalvfsg
saglmhslfemqvvwaprlanmrfaslpgghffvdrfpddtarilreflsdars