PDB entry 3ay6

View 3ay6 on RCSB PDB site
Description: Crystal structure of Bacillus megaterium glucose dehydrogenase 4 A258F mutant in complex with NADH and D-glucose
Class: oxidoreductase
Keywords: Rossmann Fold, Oxidation-reduction process, Nucleotide binding, Cytosol, OXIDOREDUCTASE
Deposited on 2011-04-29, released 2012-05-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Glucose 1-dehydrogenase 4
    Species: Bacillus megaterium [TaxId:1404]
    Gene: gdhIV
    Database cross-references and differences (RAF-indexed):
    • Uniprot P39485 (8-268)
      • expression tag (6-7)
      • engineered mutation (265)
    Domains in SCOPe 2.08: d3ay6a1, d3ay6a2
  • Chain 'B':
    Compound: Glucose 1-dehydrogenase 4
    Species: Bacillus megaterium [TaxId:1404]
    Gene: gdhIV
    Database cross-references and differences (RAF-indexed):
    • Uniprot P39485 (8-268)
      • expression tag (2-7)
      • engineered mutation (265)
    Domains in SCOPe 2.08: d3ay6b1, d3ay6b2
  • Chain 'C':
    Compound: Glucose 1-dehydrogenase 4
    Species: Bacillus megaterium [TaxId:1404]
    Gene: gdhIV
    Database cross-references and differences (RAF-indexed):
    • Uniprot P39485 (8-268)
      • expression tag (7)
      • engineered mutation (265)
    Domains in SCOPe 2.08: d3ay6c1, d3ay6c2
  • Chain 'D':
    Compound: Glucose 1-dehydrogenase 4
    Species: Bacillus megaterium [TaxId:1404]
    Gene: gdhIV
    Database cross-references and differences (RAF-indexed):
    • Uniprot P39485 (8-268)
      • expression tag (2-7)
      • engineered mutation (265)
    Domains in SCOPe 2.08: d3ay6d1, d3ay6d2
  • Heterogens: NAI, BGC, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3ay6A (A:)
mghhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkev
eeaggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkv
idtnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetl
aleyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaafla
ssqasyvtgitlfadggmtkypsfqfgrg

    Sequence, based on observed residues (ATOM records): (download) 
    >3ay6A (A:)
hhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkeveeaggq
aiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkvidtnlt
gaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetlaleyap
kgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaaflassqasy
vtgitlfadggmtkypsfqfgrg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >3ay6B (B:)
mghhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkev
eeaggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkv
idtnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetl
aleyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaafla
ssqasyvtgitlfadggmtkypsfqfgrg

    Sequence, based on observed residues (ATOM records): (download) 
    >3ay6B (B:)
hhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkevee
aggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkvid
tnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetlal
eyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaaflass
qasyvtgitlfadggmtkypsfqfgrg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >3ay6C (C:)
mghhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkev
eeaggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkv
idtnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetl
aleyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaafla
ssqasyvtgitlfadggmtkypsfqfgrg

    Sequence, based on observed residues (ATOM records): (download) 
    >3ay6C (C:)
hmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkeveeaggqa
iivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkvidtnltg
aflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetlaleyapk
girvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaaflassqasyv
tgitlfadggmtkypsfqfgrg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >3ay6D (D:)
mghhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkev
eeaggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkv
idtnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetl
aleyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaafla
ssqasyvtgitlfadggmtkypsfqfgrg

    Sequence, based on observed residues (ATOM records): (download) 
    >3ay6D (D:)
hhhhhhmytdlkdkvvvitggstglgramavrfgqeeakvvinyynneeealdakkevee
aggqaiivqgdvtkeedvvnlvqtaikefgtldvminnagvenpvpshelsldnwnkvid
tnltgaflgsreaikyfvendikgnvinmssvhemipwplfvhyaaskggmklmtetlal
eyapkgirvnnigpgamntpinaekfadpvqradvesmipmgyigkpeevaavaaflass
qasyvtgitlfadggmtkypsfqfgrg