PDB entry 3awi

View 3awi on RCSB PDB site
Description: Bifunctional tRNA modification enzyme MnmC from Escherichia coli
Deposited on 2011-03-23, released 2011-06-01
The last revision was dated 2014-11-12, with a file datestamp of 2014-11-07.
Experiment type: XRAY
Resolution: 3 Å
R-factor: 0.205
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC
    Species: Escherichia coli [TaxId:83333]
    Gene: mnmC, yfcK, b2324, JW5380
    Database cross-references and differences (RAF-indexed):
  • Heterogens: FAD, SO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >3awiA (A:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiA (A:)
leetryvflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqr
lhfisfekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwf
gdineltsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsag
fvrrglqdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggi
asallslallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfar
rfydqlpvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatn
csgitypqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqath
svvvlanghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqh
hcigasyhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhl
pmvgnvpdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqm
sdepipmdastlaalnpnrlwvrkllkg

  • Chain 'B':
    Sequence, based on SEQRES records:
    >3awiB (B:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiB (B:)
leetryvflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqr
lhfisfekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwf
gdineltsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsag
fvrrglqdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggi
asallslallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfar
rfydqlpvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatn
csgitypqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqath
svvvlanghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqh
hcigasyhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhl
pmvgnvpdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqm
sdepipmdastlaalnpnrlwvrkllkg

  • Chain 'C':
    Sequence, based on SEQRES records:
    >3awiC (C:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiC (C:)
leetryvflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqr
lhfisfekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwf
gdineltsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsag
fvrrglqdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggi
asallslallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfar
rfydqlpvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatn
csgitypqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqath
svvvlanghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqh
hcigasyhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhl
pmvgnvpdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqm
sdepipmdastlaalnpnrlwvrkllkg

  • Chain 'D':
    Sequence, based on SEQRES records:
    >3awiD (D:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiD (D:)
mkhysiqpanlefntpvsrdfddvyfsndngleetryvflggnqlevrfpehphplfvva
esgfgtglnfltlwqafdqfreahpqaqlqrlhfisfekfpltradlalahqhwpelapw
aeqlqaqwpmplpgchrllldegrvtldlwfgdineltsqlddslnqkvdawfldgfapa
knpdmwtqnlfnamarlarpggtlatftsagfvrrglqdagftmqkrkgfgrkremlcgv
meqtlplpcsapwfnrtgsskreaaiigggiasallslallrrgwqvtlycadeapalga
sgnrqgalypllskhdealnrffsnaftfarrfydqlpvkfdhdwcgvtqlgwdeksqhk
iaqmlsmdlpaelavaveanaveqitgvatncsgitypqggwlcpaeltrnvlelaqqqg
lqiyyqyqlqnlsrkddcwllnfagdqqathsvvvlanghqisrfsqtstlpvysvagqv
shipttpelaelkqvlcydgyltpqnpanqhhcigasyhrgsedtayseddqqqnrqrli
dcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnvpdyeatlveyaslaeqkdeavsa
pvfddlfmfaalgsrglcsaplcaeilaaqmsdepipmdastlaalnpnrlwvrkllkg

  • Chain 'E':
    Sequence, based on SEQRES records:
    >3awiE (E:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiE (E:)
leetryvflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqr
lhfisfekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwf
gdineltsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsag
fvrrglqdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggi
asallslallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfar
rfydqlpvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatn
csgitypqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqath
svvvlanghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqh
hcigasyhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhl
pmvgnvpdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqm
sdepipmdastlaalnpnrlwvrkllkg

  • Chain 'F':
    Sequence, based on SEQRES records:
    >3awiF (F:)
mgsshhhhhhssglvprgshmkhysiqpanlefnaegtpvsrdfddvyfsndngleetry
vflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqrlhfisf
ekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwfgdinel
tsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsagfvrrgl
qdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggiasalls
lallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfarrfydql
pvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatncsgity
pqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqathsvvvla
nghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqhhcigas
yhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhlpmvgnv
pdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqmsdepip
mdastlaalnpnrlwvrkllkgkavkag

    Sequence, based on observed residues (ATOM records):
    >3awiF (F:)
leetryvflggnqlevrfpehphplfvvaesgfgtglnfltlwqafdqfreahpqaqlqr
lhfisfekfpltradlalahqhwpelapwaeqlqaqwpmplpgchrllldegrvtldlwf
gdineltsqlddslnqkvdawfldgfapaknpdmwtqnlfnamarlarpggtlatftsag
fvrrglqdagftmqkrkgfgrkremlcgvmeqtlplpcsapwfnrtgsskreaaiigggi
asallslallrrgwqvtlycadeapalgasgnrqgalypllskhdealnrffsnaftfar
rfydqlpvkfdhdwcgvtqlgwdeksqhkiaqmlsmdlpaelavaveanaveqitgvatn
csgitypqggwlcpaeltrnvlelaqqqglqiyyqyqlqnlsrkddcwllnfagdqqath
svvvlanghqisrfsqtstlpvysvagqvshipttpelaelkqvlcydgyltpqnpanqh
hcigasyhrgsedtayseddqqqnrqrlidcfpqaqwakevdvsdkearcgvrcatrdhl
pmvgnvpdyeatlveyaslaeqkdeavsapvfddlfmfaalgsrglcsaplcaeilaaqm
sdepipmdastlaalnpnrlwvrkllkg