PDB entry 3ah6

View 3ah6 on RCSB PDB site
Description: Remarkable improvement of the heat stability of CutA1 from E.coli by rational protein designing
Class: unknown function
Keywords: CutA1, copper tolerance, stability, point mutation, UNKNOWN FUNCTION
Deposited on 2010-04-15, released 2010-08-11
The last revision prior to the SCOPe 2.05 freeze date was dated 2011-04-13, with a file datestamp of 2011-04-08.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.202
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6a_
  • Chain 'B':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6b_
  • Chain 'C':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6c_
  • Chain 'D':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488 (Start-111)
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6d_
  • Chain 'E':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6e_
  • Chain 'F':
    Compound: Divalent-cation tolerance protein cutA
    Species: Escherichia coli [TaxId:83333]
    Gene: cutA1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P69488
      • engineered mutation (10)
    Domains in SCOPe 2.05: d3ah6f_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >3ah6A (A:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6A (A:)
    tavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmilkt
    tvshqqalleclkshhpyqtpellvlpvthgdtdylswlnasl
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >3ah6B (B:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6B (B:)
    ntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmilk
    ttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnasl
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >3ah6C (C:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6C (C:)
    sntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmil
    kttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnasl
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >3ah6D (D:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6D (D:)
    tavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmilkt
    tvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

  • Chain 'E':
    Sequence, based on SEQRES records: (download)
    >3ah6E (E:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6E (E:)
    tavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmilkt
    tvshqqalleclkshhpyqtpellvlpvthgdtdylswlnasl
    

  • Chain 'F':
    Sequence, based on SEQRES records: (download)
    >3ah6F (F:)
    mldekssntavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqey
    evqmilkttvshqqalleclkshhpyqtpellvlpvthgdtdylswlnaslr
    

    Sequence, based on observed residues (ATOM records): (download)
    >3ah6F (F:)
    tavvvvlctapdeataqdlaakvlaeklaacatlipgatslyywegkleqeyevqmilkt
    tvshqqalleclkshhpyqtpellvlpvthgdtdylswlnasl