PDB entry 3a8m

View 3a8m on RCSB PDB site
Description: Crystal structure of Nitrile Hydratase mutant Y72F complexed with Trimethylacetonitrile
Class: lyase
Keywords: nitrile hydratase, Fe, Iron, Lyase, Metal-binding, Oxidation
Deposited on 2009-10-06, released 2010-04-14
The last revision prior to the SCOPe 2.01 freeze date was dated 2010-04-14, with a file datestamp of 2010-04-09.
Experiment type: XRAY
Resolution: 1.32 Å
R-factor: 0.157
AEROSPACI score: 0.75 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Nitrile hydratase subunit alpha
    Species: Rhodococcus erythropolis [TaxId:1833]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.01: d3a8ma_
  • Chain 'B':
    Compound: Nitrile hydratase subunit beta
    Species: Rhodococcus erythropolis [TaxId:1833]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P13449 (0-211)
      • engineered (71)
    Domains in SCOPe 2.01: d3a8mb_
  • Heterogens: FE, MG, TAN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >3a8mA (A:)
msvtidhttenaapaqapvsdrawalfraldgkglvpdgyvegwkktfeedfsprrgael
varawtdpefrqllltdgtaavaqygylgpqgeyivavedtptlknvivcslcsctawpi
lglpptwyksfeyrarvvreprkvlsemgteiasdieirvydttaetrymvlpqrpagte
gwsqeqlqeivtkdcligvaipqvptv

    Sequence, based on observed residues (ATOM records): (download) 
    >3a8mA (A:)
enaapaqapvsdrawalfraldgkglvpdgyvegwkktfeedfsprrgaelvarawtdpe
frqllltdgtaavaqygylgpqgeyivavedtptlknvivcslcsctawpilglpptwyk
sfeyrarvvreprkvlsemgteiasdieirvydttaetrymvlpqrpagtegwsqeqlqe
ivtkdcligvaipqvpt

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >3a8mB (B:)
mdgvhdlagvqgfgkvphtvnadigptfhaewehlpyslmfagvaelgafsvdevryvve
rmeprhymmtpfyeryvigvatlmvekgiltqdeleslaggpfplsrpsesegrpapvet
ttfevgqrvrvrdeyvpghirmpaycrgrvgtishrttekwpfpdaighgrndageepty
hvkfaaeelfgsdtdggsvvvdlfegylepaa