SCOPe 2.05: Structural Classification of Proteins

PDB entry 3a50

View 3a50 on RCSB PDB site
Description: Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound vitamin D3
Class: oxidoreductase
Keywords: Cytochrome P450, vitamin D3 hydroxylase, hemoprotein, monooxygenase, directed evolution, oxidoreductase
Deposited on 2009-07-24, released 2010-07-28
The last revision prior to the SCOPe 2.05 freeze date was dated 2010-11-17, with a file datestamp of 2010-11-12.
Experiment type: XRAY
Resolution: 2.05 Å
R-factor: 0.203
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644 (Start-402)
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.05: d3a50a_
Chain 'B':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644 (Start-402)
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.05: d3a50b_
Chain 'C':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.05: d3a50c_
Chain 'D':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.05: d3a50d_
Chain 'E':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.05: d3a50e_
Heterogens: HEM, VD3, ACT, CA, GOL, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>3a50A (A:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhrlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellglpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdmdgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfclgaqlarlegrva
igrlfadrpelalavgldelvyrrstlvrglsrmpvtmgprsalehhhhhh