SCOPe 2.08: Structural Classification of Proteins

PDB entry 3a4z

View 3a4z on RCSB PDB site
Description: Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution
Class: oxidoreductase
Keywords: Cytochrome P450, vitamin D3 hydroxylase, hemoprotein, monooxygenase, directed evolution, oxidoreductase
Deposited on 2009-07-24, released 2010-07-28
The last revision prior to the SCOPe 2.08 freeze date was dated 2010-11-17, with a file datestamp of 2010-11-12.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: 0.203
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644 (Start-402)
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.08: d3a4za_
Chain 'B':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644 (Start-402)
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.08: d3a4zb_
Chain 'C':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.08: d3a4zc_
Chain 'D':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.08: d3a4zd_
Chain 'E':
Compound: Vitamin D hydroxylase
Species: Pseudonocardia autotrophica [TaxId:2074]
Gene: vdh
Database cross-references and differences (RAF-indexed):
- Uniprot C4B644
  - engineered mutation (69)
  - engineered mutation (155)
  - engineered mutation (215)
  - engineered mutation (383)
Domains in SCOPe 2.08: d3a4ze_
Heterogens: HEM, CA, ACT, GOL, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>3a4zA (A:)
maltttgteqhdlfsgtfwqnphpayaalraedpvrklalpdgpvwlltryadvreafvd
prlskdwrhrlpedqradmpatptpmmilmdppdhtrlrklvgrsftvrrmneleprite
iadgllaglptdgpvdlmreyafqipvqvicellglpaedrddfsawssvlvddspaddk
naamgklhgylsdllerkrtepddallssllavsdmdgdrlsqeelvamamllliaghet
tvnligngvlallthpdqrkllaedpslissaveeflrfdspvsqapirftaedvtysgv
tipagemvmlglaaanrdadwmpepdrlditrdasggvffghgihfclgaqlarlegrva
igrlfadrpelalavgldelvyrrstlvrglsrmpvtmgprsalehhhhhh