PDB entry 2zqa

View 2zqa on RCSB PDB site
Description: Cefotaxime acyl-intermediate structure of class a beta-lacta Toho-1 E166A/R274N/R276N triple mutant
Class: hydrolase
Keywords: EXTENDED-SPECTRUM, ESBL, BETA-LACTAMASE, ACYL-ENZYME, COMPLEX, CEFOTAXIME, TOHO-1, HYDROLASE, Antibiotic resistance
Deposited on 2008-08-07, released 2009-07-28
The last revision prior to the SCOPe 2.01 freeze date was dated 2011-08-24, with a file datestamp of 2011-08-19.
Experiment type: XRAY
Resolution: 0.95 Å
R-factor: 0.136
AEROSPACI score: 1.07 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-lactamase toho-1
    Species: Escherichia coli [TaxId:562]
    Gene: bla
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q47066 (Start-261)
      • engineered mutation (139)
      • engineered mutation (245)
      • engineered mutation (247)
    Domains in SCOPe 2.01: d2zqaa_
  • Heterogens: PCZ, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >2zqaA (A:)
    ansvqqqlealekssggrlgvalintadnsqilyraderfamcstskvmaaaavlkqses
    dkhllnqrveikksdlvnynpiaekhvngtmtlaelgaaalqysdntamnkliahlggpd
    kvtafarslgdetfrldrtaptlntaipgdprdtttplamaqtlknltlgkalaetqraq
    lvtwlkgnttgsasiraglpkswvvgdktgsgdygttndiaviwpenhaplvlvtyftqp
    eqkaenrndilaaaakivthgf
    

    Sequence, based on observed residues (ATOM records): (download)
    >2zqaA (A:)
    nsvqqqlealekssggrlgvalintadnsqilyraderfamcstskvmaaaavlkqsesd
    khllnqrveikksdlvnynpiaekhvngtmtlaelgaaalqysdntamnkliahlggpdk
    vtafarslgdetfrldrtaptlntaipgdprdtttplamaqtlknltlgkalaetqraql
    vtwlkgnttgsasiraglpkswvvgdktgsgdygttndiaviwpenhaplvlvtyftqpe
    qkaenrndilaaaakivthgf