PDB entry 2z1i

View 2z1i on RCSB PDB site
Description: Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T40E/Q72H/Q76K/Q80E/T92K/Q105K/Q113R/Q115K)
Class: hydrolase
Keywords: RNase HI, thermostability, surface-charge residue, HYDROLASE
Deposited on 2007-05-08, released 2007-11-13
The last revision prior to the SCOPe 2.08 freeze date was dated 2009-02-24, with a file datestamp of 2009-02-03.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.211
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (3)
      • engineered (39)
      • engineered (71)
      • engineered (75)
      • engineered (79)
      • engineered (91)
      • engineered (104)
      • engineered (112)
      • engineered (114)
    Domains in SCOPe 2.08: d2z1ia_
  • Chain 'B':
    Compound: ribonuclease hi
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A7Y4 (0-End)
      • engineered (3)
      • engineered (39)
      • engineered (71)
      • engineered (75)
      • engineered (79)
      • engineered (91)
      • engineered (104)
      • engineered (112)
      • engineered (114)
    Domains in SCOPe 2.08: d2z1ib_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2z1iA (A:)
mlkrveiftdgsclgnpgpggygailryrgrektfsagyerttnnrmelmaaivalealk
ehcevilstdshyvrkgitewihnwkkrgwkkadkkpvknvdlwkrldaalgrhkikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >2z1iA (A:)
mlkrveiftdgsclgnpgpggygailryrgrektfsagyerttnnrmelmaaivalealk
ehcevilstdshyvrkgitewihnwkkrgwkkadkkpvknvdlwkrldaalgrhkikwew
vkghaghpenercdelaraaamnptledtgy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2z1iB (B:)
mlkrveiftdgsclgnpgpggygailryrgrektfsagyerttnnrmelmaaivalealk
ehcevilstdshyvrkgitewihnwkkrgwkkadkkpvknvdlwkrldaalgrhkikwew
vkghaghpenercdelaraaamnptledtgyqvev

    Sequence, based on observed residues (ATOM records): (download) 
    >2z1iB (B:)
mlkrveiftdgsclgnpgpggygailryrgrektfsagyerttnnrmelmaaivalealk
ehcevilstdshyvrkgitewihnwkkrgwkkadkkpvknvdlwkrldaalgrhkikwew
vkghaghpenercdelaraaamnptledtgy