PDB entry 2y12

View 2y12 on RCSB PDB site
Description: The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome
Deposited on 2010-12-07, released 2011-03-09
The last revision was dated 2014-12-10, with a file datestamp of 2014-12-05.
Experiment type: XRAY
Resolution: 3.1 Å
R-factor: 0.2384
AEROSPACI score: 0.12 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 16s rRNA
    Species: THERMUS THERMOPHILUS
  • Chain 'B':
    Compound: 30S ribosomal protein S2
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: 30S ribosomal protein S3
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: 30S ribosomal protein S4
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: 30S ribosomal protein S5
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: 30S ribosomal protein S6
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: 30S ribosomal protein S7
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: 30S ribosomal protein S8
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: 30S ribosomal protein S9
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: 30S ribosomal protein S10
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: 30S ribosomal protein S11
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: 30S ribosomal protein S12
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: 30S ribosomal protein S13
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: 30S ribosomal protein S14
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: 30S ribosomal protein S15
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'P':
    Compound: 30S ribosomal protein S16
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'Q':
    Compound: 30S ribosomal protein S17
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: 30S ribosomal protein S18
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'S':
    Compound: 30S ribosomal protein S19
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'T':
    Compound: 30S ribosomal protein S20
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'U':
    Compound: 30S ribosomal protein Thx
    Species: THERMUS THERMOPHILUS
    Database cross-references and differences (RAF-indexed):
  • Chain 'V':
    Compound: e-site tRNA phe or p-site tRNA phe
    Species: ESCHERICHIA COLI
  • Chain 'W':
    Compound: e-site tRNA phe or p-site tRNA phe
    Species: ESCHERICHIA COLI
  • Chain 'X':
    Compound: mRNA
    Species: Escherichia coli, synthetic
  • Chain 'Y':
    Compound: a-site tRNA g24a trp-tRNA trp
    Species: ESCHERICHIA COLI
  • Chain 'Z':
    Compound: elongation factor tu
    Species: Thermus thermophilus
    Database cross-references and differences (RAF-indexed):
  • Heterogens: ZN, GDP, KIR

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records:
    >2y12B (B:)
mpveitvkelleagvhfgherkrwnpkfaryiyaerngihiidlqktmeelertfrfied
lamrggtilfvgtkkqaqdivrmeaeragmpyvnqrwlggmltnfktisqrvhrleelea
lfaspeieerpkkeqvrlkhelerlqkylsgfrllkrlpdaifvvdptkeaiavrearkl
fipvialadtdsdpdlvdyiipgnddairsiqlilsravdliiqarggvvepspsyalvq
eaeatetpegesevea

    Sequence, based on observed residues (ATOM records):
    >2y12B (B:)
vkelleagvhfgherkrwnpkfaryiyaerngihiidlqktmeelertfrfiedlamrgg
tilfvgtkkqaqdivrmeaeragmpyvnqrwlggmltnfktisqrvhrleelealfaspe
ieerpkkeqvrlkhelerlqkylsgfrllkrlpdaifvvdptkeaiavrearklfipvia
ladtdsdpdlvdyiipgnddairsiqlilsravdliiqarggvvepspsyalvq

  • Chain 'C':
    Sequence, based on SEQRES records:
    >2y12C (C:)
mgnkihpigfrlgitrdwesrwyagkkqyrhllledqrirgllekelysaglarvdiera
adnvavtvhvakpgvvigrggerirvlreelakltgknvalnvqevqnpnlsaplvaqrv
aeqierrfavrraikqavqrvmesgakgakvivsgriggaeqartewaaqgrvplhtlra
nidygfalarttygvlgvkayiflgeviggqkpkarpelpkaeerprrrrpavrvkkee

    Sequence, based on observed residues (ATOM records):
    >2y12C (C:)
gnkihpigfrlgitrdwesrwyagkkqyrhllledqrirgllekelysaglarvdieraa
dnvavtvhvakpgvvigrggerirvlreelakltgknvalnvqevqnpnlsaplvaqrva
eqierrfavrraikqavqrvmesgakgakvivsgriggaeqartewaaqgrvplhtlran
idygfalarttygvlgvkayiflgev

  • Chain 'D':
    Sequence, based on SEQRES records:
    >2y12D (D:)
mgryigpvcrlcrregvklylkgercyspkcamerrpyppgqhgqkrarrpsdyavrlre
kqklrriygiserqfrnlfeeaskkkgvtgsvflgllesrldnvvyrlgfavsrrqarql
vrhghitvngrrvdlpsyrvrpgdeiavaeksrnlelirqnleamkgrkvgpwlsldveg
mkgkflrlpdredlalpvneqlviefysr

    Sequence, based on observed residues (ATOM records):
    >2y12D (D:)
gryigpvcrlcrregvklylkgercyspkcamerrpyppgqhgqkrarrpsdyavrlrek
qklrriygiserqfrnlfeeaskkkgvtgsvflgllesrldnvvyrlgfavsrrqarqlv
rhghitvngrrvdlpsyrvrpgdeiavaeksrnlelirqnleamkgrkvgpwlsldvegm
kgkflrlpdredlalpvneqlviefysr

  • Chain 'E':
    Sequence, based on SEQRES records:
    >2y12E (E:)
mpetdfeekmilirrtarmqaggrrfrfgalvvvgdrqgrvglgfgkapevplavqkagy
yarrnmvevplqngtipheievefgaskivlkpaapgtgviagavprailelagvtdilt
kelgsrnpiniayatmealrqlrtkadverlrkgeahaqaqg

    Sequence, based on observed residues (ATOM records):
    >2y12E (E:)
dfeekmilirrtarmqaggrrfrfgalvvvgdrqgrvglgfgkapevplavqkagyyarr
nmvevplqngtipheievefgaskivlkpaapgtgviagavprailelagvtdiltkelg
srnpiniayatmealrqlrtkadverlrkg

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records:
    >2y12F (F:)
mrryevnivlnpnldqsqlalekeiiqralenygarvekveelglrrlaypiakdpqgyf
lwyqvempedrvndlarelrirdnvrrvmvvksqepflana

  • Chain 'G':
    Sequence, based on SEQRES records:
    >2y12G (G:)
marrrraevrqlqpdlvygdvlvtafinkimrdgkknlaarifydackiiqektgqeplk
vfkqavenvkprmevrsrrvgganyqvpmevsprrqqslalrwlvqaanqrperraavri
ahelmdaaegkggavkkkedvermaeanrayahyrw

    Sequence, based on observed residues (ATOM records):
    >2y12G (G:)
arrrraevrqlqpdlvygdvlvtafinkimrdgkknlaarifydackiiqektgqeplkv
fkqavenvkprmevrsrrvgganyqvpmevsprrqqslalrwlvqaanqrperraavria
helmdaaegkggavkkkedvermaeanrayahyrw

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records:
    >2y12H (H:)
mltdpiadmltrirnatrvykestdvpasrfkeeilrilaregfikgyervdvdgkpylr
vylkygprrqgpdprpeqvihhirriskpgrrvyvgvkeiprvrrglgiailstskgvlt
drearklgvggelicevw

  • Chain 'I':
    Sequence, based on SEQRES records:
    >2y12I (I:)
meqyygtgrrkeavarvflrpgngkvtvngqdfneyfqglvravaaleplravdalghfd
ayitvrgggksgqidaiklgiaralvqynpdyraklkplgfltrdarvverkkygkhkar
rapqyskr

    Sequence, based on observed residues (ATOM records):
    >2y12I (I:)
eqyygtgrrkeavarvflrpgngkvtvngqdfneyfqglvravaaleplravdalghfda
yitvrgggksgqidaiklgiaralvqynpdyraklkplgfltrdarvverkkygkhkarr
apqyskr

  • Chain 'J':
    Sequence, based on SEQRES records:
    >2y12J (J:)
mpkiriklrgfdhktldasaqkiveaarrsgaqvsgpiplptrvrrftvirgpfkhkdsr
ehfelrthnrlvdiinpnrktieqlmtldlptgveieiktvgggr

    Sequence, based on observed residues (ATOM records):
    >2y12J (J:)
kiriklrgfdhktldasaqkiveaarrsgaqvsgpiplptrvrrftvirgpfkhkdsreh
felrthnrlvdiinpnrktieqlmtldlptgveieikt

  • Chain 'K':
    Sequence, based on SEQRES records:
    >2y12K (K:)
makkpskkkvkrqvasgrayihasynntivtitdpdgnpitwssggvigykgsrkgtpya
aqlaaldaakkamaygmqsvdvivrgtgagreqairalqasglqvksivddtpvphngcr
pkkkfrkas

    Sequence, based on observed residues (ATOM records):
    >2y12K (K:)
krqvasgrayihasynntivtitdpdgnpitwssggvigykgsrkgtpyaaqlaaldaak
kamaygmqsvdvivrgtgagreqairalqasglqvksivddtpvphngcrpkkkfrkas

  • Chain 'L':
    Sequence, based on SEQRES records:
    >2y12L (L:)
mptinqlvrkgrekvrkkskvpalkgapfrrgvctvvrtvtpkkpnsalrkvakvrltsg
yevtayipgeghnlqehsvvlirggrvkdlpgvryhivrgvydaagvkdrkksrskygtk
kpkeaaktaakk

    Sequence, based on observed residues (ATOM records):
    >2y12L (L:)
ptinqlvrkgrekvrkkskvpalkgapfrrgvctvvrtvtpkkpnsalrkvakvrltsgy
evtayipgeghnlqehsvvlirggrvkdlpgvryhivrgvydaagvkdrkksrskygtkk
pkea

  • Chain 'M':
    Sequence, based on SEQRES records:
    >2y12M (M:)
mariagveiprnkrvdvaltyiygigkarakealektginpatrvkdlteaevvrlreyv
entwklegelraevaanikrlmdigcyrglrhrrglpvrgqrtrtnartrkgprktvagk
kkaprk

    Sequence, based on observed residues (ATOM records):
    >2y12M (M:)
ariagveiprnkrvdvaltyiygigkarakealektginpatrvkdlteaevvrlreyve
ntwklegelraevaanikrlmdigcyrglrhrrglpvrgqrtrtnartrkgprktvagkk
kapr

  • Chain 'N':
    Sequence, based on SEQRES records:
    >2y12N (N:)
markaliekakrtpkfkvraytrcvrcgrarsvyrffglcriclrelahkgqlpgvrkas
w

    Sequence, based on observed residues (ATOM records):
    >2y12N (N:)
arkaliekakrtpkfkvraytrcvrcgrarsvyrffglcriclrelahkgqlpgvrkasw

  • Chain 'O':
    Sequence, based on SEQRES records:
    >2y12O (O:)
mpitkeekqkviqefarfpgdtgstevqvalltlrinrlsehlkvhkkdhhshrgllmmv
gqrrrllrylqredperyralieklgirg

    Sequence, based on observed residues (ATOM records):
    >2y12O (O:)
pitkeekqkviqefarfpgdtgstevqvalltlrinrlsehlkvhkkdhhshrgllmmvg
qrrrllrylqredperyralieklgirg

  • Chain 'P':
    Sequence, based on SEQRES records:
    >2y12P (P:)
mvkirlarfgskhnphyrivvtdarrkrdgkyiekigyydprkttpdwlkvdverarywl
svgaqptdtarrllrqagvfrqearega

    Sequence, based on observed residues (ATOM records):
    >2y12P (P:)
mvkirlarfgskhnphyrivvtdarrkrdgkyiekigyydprkttpdwlkvdverarywl
svgaqptdtarrllrqagvfrqe

  • Chain 'Q':
    Sequence, based on SEQRES records:
    >2y12Q (Q:)
mpkkvltgvvvsdkmqktvtvlverqfphplygkvikrskkylahdpeekyklgdvveii
esrpiskrkrfrvlrlvesgrmdlvekylirrqnyeslskrggka

    Sequence, based on observed residues (ATOM records):
    >2y12Q (Q:)
pkkvltgvvvsdkmqktvtvlverqfphplygkvikrskkylahdpeekyklgdvveiie
srpiskrkrfrvlrlvesgrmdlvekylirrqnyeslsk

  • Chain 'R':
    Sequence, based on SEQRES records:
    >2y12R (R:)
mstknakpkkeaqrrpsrkakvkatlgefdlrdyrnvevlkrflsetgkilprrrtglsa
keqrilaktikrarilgllpfteklvrk

    Sequence, based on observed residues (ATOM records):
    >2y12R (R:)
kakvkatlgefdlrdyrnvevlkrflsetgkilprrrtglsakeqrilaktikrarilgl
lpfteklvrk

  • Chain 'S':
    Sequence, based on SEQRES records:
    >2y12S (S:)
mprslkkgvfvddhllekvlelnakgekrliktwsrrstivpemvghtiavyngkqhvpv
yitenmvghklgefaptrtyrghgkeakatkkk

    Sequence, based on observed residues (ATOM records):
    >2y12S (S:)
slkkgvfvddhllekvlelnakgekrliktwsrrstivpemvghtiavyngkqhvpvyit
enmvghklgefaptrtyr

  • Chain 'T':
    Sequence, based on SEQRES records:
    >2y12T (T:)
maqkkpkrnlsalkrhrqslkrrlrnkakksaiktlskkaiqlaqegkaeealkimrkae
slidkaakgstlhknaaarrksrlmrkvrqlleaagapliggglsa

    Sequence, based on observed residues (ATOM records):
    >2y12T (T:)
rnlsalkrhrqslkrrlrnkakksaiktlskkaiqlaqegkaeealkimrkaeslidkaa
kgstlhknaaarrksrlmrkvrqlleaagapliggglsa

  • Chain 'U':
    Sequence, based on SEQRES records:
    >2y12U (U:)
mgkgdrrtrrgkiwrgtygkyrprkkk

    Sequence, based on observed residues (ATOM records):
    >2y12U (U:)
gkgdrrtrrgkiwrgtygkyrprk

  • Chain 'V':
    No sequence available.

  • Chain 'W':
    No sequence available.

  • Chain 'X':
    No sequence available.

  • Chain 'Y':
    No sequence available.

  • Chain 'Z':
    Sequence, based on SEQRES records:
    >2y12Z (Z:)
akgefvrtkphvnvgtighvdhgkttltaaltyvaaaenpnvevkdygdidkapeerarg
itintahveyetakrhyshvdcpghadyiknmitgaaqmdgailvvsaadgpmpqtrehi
llarqvgvpyivvfmnkvdmvddpelldlvemevrdllnqyefpgdevpvirgsallale
qmhrnpktrrgenewvdkiwelldaideyiptpvrdvdkpflmpvedvftitgrgtvatg
riergkvkvgdeveivglapetrrtvvtgvemhrktlqegiagdnvgvllrgvsreever
gqvlakpgsitphtkfeasvyvlkkeeggrhtgffsgyrpqfyfrttdvtgvvqlppgve
mvmpgdnvtftvelikpvaleeglrfaireggrtvgagvvtkile

    Sequence, based on observed residues (ATOM records):
    >2y12Z (Z:)
akgefvrtkphvnvgtighvdhgkttltaaltyvaaaenpnvintahveyetakrhyshv
dcpghadyiknmitgaaqmdgailvvsaadgpmpqtrehillarqvgvpyivvfmnkvdm
vddpelldlvemevrdllnqyefpgdevpvirgsallaleqmhrnpktrrgenewvdkiw
elldaideyiptpvrdvdkpflmpvedvftitgrgtvatgriergkvkvgdeveivglap
etrrtvvtgvemhrktlqegiagdnvgvllrgvsreevergqvlakpgsitphtkfeasv
yvlkkeeggrhtgffsgyrpqfyfrttdvtgvvqlppgvemvmpgdnvtftvelikpval
eeglrfaireggrtvgagvvtkile