PDB entry 2xfw

View 2xfw on RCSB PDB site
Description: Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate in crystal form III
Class: lyase
Keywords: lyase
Deposited on 2010-05-28, released 2010-09-22
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-03-13, with a file datestamp of 2013-03-08.
Experiment type: XRAY
Resolution: 1.65 Å
R-factor: 0.15772
AEROSPACI score: 0.62 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminic acid lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot B7M0T7 (8-303)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2xfwa1, d2xfwa2
  • Chain 'B':
    Compound: n-acetylneuraminic acid lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot B7M0T7 (8-End)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2xfwb1, d2xfwb2
  • Chain 'C':
    Compound: n-acetylneuraminic acid lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot B7M0T7 (8-303)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2xfwc1, d2xfwc2
  • Chain 'D':
    Compound: n-acetylneuraminic acid lyase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot B7M0T7 (8-303)
      • expression tag (5-7)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2xfwd1, d2xfwd2
  • Heterogens: PYR, 1PE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2xfwA (A:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2xfwA (A:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2xfwB (B:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2xfwB (B:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2xfwC (C:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2xfwC (C:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2xfwD (D:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2xfwD (D:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqerg