PDB entry 2xed

View 2xed on RCSB PDB site
Description: nocardia farcinica maleate cis-trans isomerase c194s mutant with a covalently bound succinylcysteine intermediate
Deposited on 2010-05-13, released 2010-08-18
The last revision was dated 2011-06-15, with a file datestamp of 2011-06-10.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: 0.22402
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: putative maleate isomerase
    Species: NOCARDIA FARCINICA [TaxId:247156]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5YXQ1 (22-End)
      • expression tag (18-21)
      • engineered mutation (215)
  • Chain 'B':
    Compound: putative maleate isomerase
    Species: NOCARDIA FARCINICA [TaxId:247156]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5YXQ1 (22-End)
      • engineered mutation (215)
  • Chain 'C':
    Compound: putative maleate isomerase
    Species: NOCARDIA FARCINICA [TaxId:247156]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5YXQ1
      • engineered mutation (215)
  • Chain 'D':
    Compound: putative maleate isomerase
    Species: NOCARDIA FARCINICA [TaxId:247156]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5YXQ1
      • engineered mutation (215)
  • Heterogens: SIN, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >2xedA (A:)
mgsshhhhhhssglevlfqgpamgirriglvvpssnvtvetempallsrhpgaefsfhst
rmrmhtvspeglaamnaqrercvleiadaapevilyaclvavmvggpgehhrvesavaeq
latggsqalvrssagalveglraldaqrvalvtpymrplaekvvayleaegftisdwral
evadntevgcipgeqvmaaarsldlsevdalviscavqmpslplvetaerefgipvlsaa
tagaysilrsldlpvavpgagrllrqdsavtas

    Sequence, based on observed residues (ATOM records):
    >2xedA (A:)
qgpamgirriglvvpssnvtvetempallsrhpgaefsfhstrmrmhtvspeglaamnaq
rercvleiadaapevilyaclvavmvggpgehhrvesavaeqlatggsqalvrssagalv
eglraldaqrvalvtpymrplaekvvayleaegftisdwralevadntevgcipgeqvma
aarsldlsevdalviscavqmpslplvetaerefgipvlsaatagaysilrsldlpvavp
gagrllrqdsav

  • Chain 'B':
    Sequence, based on SEQRES records:
    >2xedB (B:)
mgsshhhhhhssglevlfqgpamgirriglvvpssnvtvetempallsrhpgaefsfhst
rmrmhtvspeglaamnaqrercvleiadaapevilyaclvavmvggpgehhrvesavaeq
latggsqalvrssagalveglraldaqrvalvtpymrplaekvvayleaegftisdwral
evadntevgcipgeqvmaaarsldlsevdalviscavqmpslplvetaerefgipvlsaa
tagaysilrsldlpvavpgagrllrqdsavtas

    Sequence, based on observed residues (ATOM records):
    >2xedB (B:)
mgirriglvvpssnvtvetempallsrhpgaefsfhstrmrmhtvspeglaamnaqrerc
vleiadaapevilyaclvavmvggpgehhrvesavaeqlatggsqalvrssagalveglr
aldaqrvalvtpymrplaekvvayleaegftisdwralevadntevgcipgeqvmaaars
ldlsevdalviscavqmpslplvetaerefgipvlsaatagaysilrsldlpvavpgagr
llrqds

  • Chain 'C':
    Sequence, based on SEQRES records:
    >2xedC (C:)
mgsshhhhhhssglevlfqgpamgirriglvvpssnvtvetempallsrhpgaefsfhst
rmrmhtvspeglaamnaqrercvleiadaapevilyaclvavmvggpgehhrvesavaeq
latggsqalvrssagalveglraldaqrvalvtpymrplaekvvayleaegftisdwral
evadntevgcipgeqvmaaarsldlsevdalviscavqmpslplvetaerefgipvlsaa
tagaysilrsldlpvavpgagrllrqdsavtas

    Sequence, based on observed residues (ATOM records):
    >2xedC (C:)
girriglvvpssnvtvetempallsrhpgaefsfhstrmrmhtvspeglaamnaqrercv
leiadaapevilyaclvavmvggpgehhrvesavaeqlatggsqalvrssagalveglra
ldaqrvalvtpymrplaekvvayleaegftisdwralevadntevgcipgeqvmaaarsl
dlsevdalviscavqmpslplvetaerefgipvlsaatagaysilrsldlpvavpgagrl
lrqds

  • Chain 'D':
    Sequence, based on SEQRES records:
    >2xedD (D:)
mgsshhhhhhssglevlfqgpamgirriglvvpssnvtvetempallsrhpgaefsfhst
rmrmhtvspeglaamnaqrercvleiadaapevilyaclvavmvggpgehhrvesavaeq
latggsqalvrssagalveglraldaqrvalvtpymrplaekvvayleaegftisdwral
evadntevgcipgeqvmaaarsldlsevdalviscavqmpslplvetaerefgipvlsaa
tagaysilrsldlpvavpgagrllrqdsavtas

    Sequence, based on observed residues (ATOM records):
    >2xedD (D:)
girriglvvpssnvtvetempallsrhpgaefsfhstrmrmhtvspeglaamnaqrercv
leiadaapevilyaclvavmvggpgehhrvesavaeqlatggsqalvrssagalveglra
ldaqrvalvtpymrplaekvvayleaegftisdwralevadntevgcipgeqvmaaarsl
dlsevdalviscavqmpslplvetaerefgipvlsaatagaysilrsldlpvavpgagrl
lrqds