PDB entry 2wvs

View 2wvs on RCSB PDB site
Description: crystal structure of an alpha-l-fucosidase gh29 trapped covalent intermediate from bacteroides thetaiotaomicron in complex with 2- fluoro-fucosyl fluoride using an e288q mutant
Deposited on 2009-10-20, released 2010-02-02
The last revision was dated 2020-07-29, with a file datestamp of 2020-06-29.
Experiment type: XRAY
Resolution: 2.19 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alpha-l-fucosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8A3I4 (Start-442)
      • engineered mutation (257)
  • Chain 'B':
    Compound: alpha-l-fucosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8A3I4
      • engineered mutation (257)
  • Chain 'C':
    Compound: alpha-l-fucosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8A3I4
      • engineered mutation (257)
  • Chain 'D':
    Compound: alpha-l-fucosidase
    Species: BACTEROIDES THETAIOTAOMICRON [TaxId:226186]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8A3I4
      • engineered mutation (257)
  • Heterogens: FUF, SO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >2wvsA (A:)
eakkeiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlk
swakvpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkyt
vantpykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftd
nqlkelatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgk
rhfdsngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktp
ievidrivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfe
kqdwgyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrney
nvsvpkknpgepyviqlkvraak

    Sequence, based on observed residues (ATOM records):
    >2wvsA (A:)
eiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlkswak
vpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkytvant
pykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftdnqlk
elatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgkrhfd
sngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktpievi
drivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfekqdw
gyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrneynvsv
pkknpgepyviqlkvraak

  • Chain 'B':
    Sequence, based on SEQRES records:
    >2wvsB (B:)
eakkeiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlk
swakvpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkyt
vantpykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftd
nqlkelatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgk
rhfdsngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktp
ievidrivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfe
kqdwgyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrney
nvsvpkknpgepyviqlkvraak

    Sequence, based on observed residues (ATOM records):
    >2wvsB (B:)
eiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlkswak
vpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkytvant
pykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftdnqlk
elatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgkrhfd
sngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktpievi
drivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfekqdw
gyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrneynvsv
pkknpgepyviqlkvra

  • Chain 'C':
    Sequence, based on SEQRES records:
    >2wvsC (C:)
eakkeiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlk
swakvpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkyt
vantpykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftd
nqlkelatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgk
rhfdsngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktp
ievidrivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfe
kqdwgyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrney
nvsvpkknpgepyviqlkvraak

    Sequence, based on observed residues (ATOM records):
    >2wvsC (C:)
eiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlkswak
vpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkytvant
pykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftdnqlk
elatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgkrhfd
sngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktpievi
drivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfekqdw
gyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrneynvsv
pkknpgepyviqlkvraa

  • Chain 'D':
    Sequence, based on SEQRES records:
    >2wvsD (D:)
eakkeiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlk
swakvpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkyt
vantpykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftd
nqlkelatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgk
rhfdsngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktp
ievidrivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfe
kqdwgyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrney
nvsvpkknpgepyviqlkvraak

    Sequence, based on observed residues (ATOM records):
    >2wvsD (D:)
eiplkygatnegkrqdpamqkfrdnrlgafihwglyaipggewngkvyggaaewlkswak
vpadewlklmdqwnptkfdakkwakmakemgtkyvkittkhhegfclwpskytkytvant
pykrdilgelvkayndegidvhfyfsvmdwsnpdyrydikskedsiafsrfleftdnqlk
elatryptvkdfwfdgtwdasvkkngwwtahaeqmlkelvpgvainsrlraddkgkrhfd
sngrlmgdyesgyqrrlpdpvkdlkvtqwdweacmtipenqwgyhkdwslsyvktpievi
drivhavsmggnmvvnfgpqadgdfrpeekamataigkwmnrygkavyacdyagfekqdw
gyytrgkndevymvvfnqpyserlivktpkgitvekatllttgeditvvettrneynvsv
pkknpgepyviqlkvraa