PDB entry 2wnz

View 2wnz on RCSB PDB site
Description: structure of the e192n mutant of e. coli n-acetylneuraminic acid lyase in complex with pyruvate in space group p21 crystal form I
Class: lyase
Keywords: substrate specificity, carbohydrate metabolism, directed evolution, protein engineering, lyase, aldolase, schiff base
Deposited on 2009-07-21, released 2010-08-25
The last revision prior to the SCOPe 2.08 freeze date was dated 2010-11-17, with a file datestamp of 2010-11-12.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: 0.19025
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-303)
      • expression tag (2-7)
      • conflict (198)
    Domains in SCOPe 2.08: d2wnza1, d2wnza2
  • Chain 'B':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • conflict (198)
    Domains in SCOPe 2.08: d2wnzb_
  • Chain 'C':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • conflict (198)
    Domains in SCOPe 2.08: d2wnzc_
  • Chain 'D':
    Compound: n-acetylneuraminate lyase
    Species: ESCHERICHIA COLI [TaxId:469008]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6L4 (8-End)
      • expression tag (5-7)
      • conflict (198)
    Domains in SCOPe 2.08: d2wnzd1, d2wnzd2
  • Heterogens: 2OP, LAC, ETX, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wnzA (A:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnzA (A:)
hhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqs
lsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsf
eehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqme
qirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqkl
qtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqe
rg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wnzB (B:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnzB (B:)
atnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereq
vleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdh
yraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqirreh
pdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnk
vidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2wnzC (C:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnzC (C:)
atnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslsereq
vleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeehcdh
yraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqirreh
pdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqtecnk
vidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqer

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2wnzD (D:)
mehhhhhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafv
qslsereqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypf
sfeehcdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyq
meqirrehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaq
klqtecnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlm
qerg

    Sequence, based on observed residues (ATOM records): (download) 
    >2wnzD (D:)
hhhatnlrgvmaalltpfdqqqaldkaslrrlvqfniqqgidglyvggstgeafvqslse
reqvleivaeeakgkikliahvgcvstaesqqlaasakrygfdavsavtpfyypfsfeeh
cdhyraiidsadglpmvvynipalsgvkltldqintlvtlpgvgalxqtsgdlyqmeqir
rehpdlvlyngydnifasgllagadggigstynimgwryqgivkalkegdiqtaqklqte
cnkvidlliktgvfrglktvlhymdvvsvplcrkpfgpvdekylpelkalaqqlmqe