PDB entry 2wku

View 2wku on RCSB PDB site
Description: biosynthetic thiolase from z. ramigera. the n316h mutant.
Class: transferase
Keywords: acyltransferase, phb biosynthesis, transferase, thiolase fold
Deposited on 2009-06-18, released 2009-11-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-11-02, with a file datestamp of 2011-10-28.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.204
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKU (10-10)
    Domains in SCOPe 2.08: d2wkua1, d2wkua2
  • Chain 'B':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKU (10-10)
    Domains in SCOPe 2.08: d2wkub1, d2wkub2
  • Chain 'C':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKU (10-10)
    Domains in SCOPe 2.08: d2wkuc1, d2wkuc2
  • Chain 'D':
    Compound: Acetyl-CoA acetyltransferase
    Species: ZOOGLOEA RAMIGERA [TaxId:350]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07097 (11-391)
      • see remark 999 (128)
      • engineered mutation (315)
    • PDB 2WKU (10-10)
    Domains in SCOPe 2.08: d2wkud1, d2wkud2
  • Heterogens: SO4, DNO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2wkuA (A:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wkuA (A:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2wkuB (B:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wkuB (B:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2wkuC (C:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wkuC (C:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2wkuD (D:)
stpsiviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpa
gegqnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesms
maphcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafav
asqnkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegt
vtagnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkal
eragwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilnt
llfemkrrgarkglatlcigggmgvamciesl

    Sequence, based on observed residues (ATOM records): (download) 
    >2wkuD (D:)
siviasaartavgsfngafantpahelgatvisavleragvaagevnevilgqvlpageg
qnparqaamkagvpqeatawgmnqlcgsglravalgmqqiatgdasiivaggmesmsmap
hcahlrggvkmgdfkmidtmikdgltdafygyhmgttaenvakqwqlsrdeqdafavasq
nkaeaaqkdgrfkdeivpfivkgrkgditvdadeyirhgatldsmaklrpafdkegtvta
gnasglndgaaaallmseaeasrrgiqplgrivswatvgvdpkvmgtgpipasrkalera
gwkigdldlveaheafaaqacavnkdlgwdpsivnvnggaiaighpigasgarilntllf
emkrrgarkglatlcigggmgvamciesl