PDB entry 2w74

View 2w74 on RCSB PDB site
Description: mutant (k220r) of the hsdr subunit of the ecor124i restriction enzyme in complex with atp
Deposited on 2008-12-20, released 2009-12-29
The last revision was dated 2014-03-26, with a file datestamp of 2014-03-21.
Experiment type: XRAY
Resolution: 2.74 Å
R-factor: 0.246
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'B':
    Compound: type I restriction enzyme ecor124ii r protein
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10486 (0-874)
      • engineered mutation (208)
  • Chain 'D':
    Compound: type I restriction enzyme ecor124ii r protein
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P10486
      • engineered mutation (208)
  • Heterogens: ATP, MG, PO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'B':
    Sequence, based on SEQRES records:
    >2w74B (B:)
nnfivldkyikaeptgdsyqsesdlereliqdlrnqgyefisvksqsamlanvreqlqnl
ngvvfndsewrrfteqyldnpsdgildktrkihidyicdfifdderleniylidkknlmr
nkvqiiqqfeqagshanrydvtilvnglplvqielkkrgvaireafnqihryskesfnse
nslfkylqlfvisngtdtryfanttkrdrnsfdftmnwaksdntlikdlkdftatcfqkh
tllnvlvnysvfdssqtllvmrpyqiaaterilwkikssftaknwskpesggyiwhttgs
gktltsfkaarlateldfidkvffvvdrkdldyqtmkeyqrfspdsvngsentaglkrnl
dkddnkiivttiqklnnlmkaesdlpvynqqvvfifdechrsqfgeaqknlkkkfkryyq
fgftgtpifpenalgsettasvfgrelhsyvitdairdekvlkfkvdyndvrpqfkslet
etdekklsaaenqqaflhpmriqeitqyilnnfrqkthrtfpgskgfnamlavssvdaak
ayyatfkrlqeeaanksatykplriatifsfaaneeqnaigeisdetfdtsamdssakef
ldaaireynshfktnfstdsngfqnyyrdlaqrvknqdidllivvgmfltgfdaptlntl
fvdknlryhglmqafsrtnriydatktfgnivtfrdlerstidaitlfgdkntknvvlek
syteymegftdaatgeakrgfmtvvseleqrfpdptsiesekekkdfvklfgeylraeni
lqnydefatlkalqqidlsdpvavekfkaehyvddekfaelqtirlpadrkiqdyrsayn
dirdwqrrekeaekkeksttdwddvvfevdllksq

    Sequence, based on observed residues (ATOM records):
    >2w74B (B:)
nnfivldkyikaeptgdsyqsesdlereliqdlrnqgyefisvksqsamlanvreqlqnl
ngvvfndsewrrfteqyldnpsdgildktrkihidyicdfifdderleniylidkknlmr
nkvqiiqqfrydvtilvnglplvqielkkrgvaireafnqisenslfkylqlfvisngtd
tryfanttkrdrnsfdftmnwaksdntlikdlkdftatcfqkhtllnvlvnysvfdssqt
llvmrpyqiaaterilwkikssftaknwskpesggyiwhttgsgktltsfkaarlateld
fidkvffvvdrkdldyqtmkeyqrfspdsvngsentaglkrnldkddnkiivttiqklnn
lmkaesdlpvynqqvvfifdechrsqfgeaqknlkkkfkryyqfgftgtpifpenalgse
ttasvfgrelhsyvitdairdekvlkfkvdyndvrpqfksleteriqeitqyilnnfrqk
thrtfpgskgfnamlavssvdaakayyatfkplriatifsfageisdetfdtakefldaa
ireynshfktnfstdsngfqnyyrdlaqrvknqdidllivvgmfltgfdaptlntlfvdk
nlryhglmqafsrtnriydatktfgnivtfrdlerstidaitlfgdkntknvvleksyte
ymegftdgeakrgfmtvvseleqrfpdptsiesekekkdfvklfgeylraenilqnydef
atlkalfaelqtirlpadrkiqdyrsayndirdwqrrwddvvfevdllksq

  • Chain 'D':
    Sequence, based on SEQRES records:
    >2w74D (D:)
nnfivldkyikaeptgdsyqsesdlereliqdlrnqgyefisvksqsamlanvreqlqnl
ngvvfndsewrrfteqyldnpsdgildktrkihidyicdfifdderleniylidkknlmr
nkvqiiqqfeqagshanrydvtilvnglplvqielkkrgvaireafnqihryskesfnse
nslfkylqlfvisngtdtryfanttkrdrnsfdftmnwaksdntlikdlkdftatcfqkh
tllnvlvnysvfdssqtllvmrpyqiaaterilwkikssftaknwskpesggyiwhttgs
gktltsfkaarlateldfidkvffvvdrkdldyqtmkeyqrfspdsvngsentaglkrnl
dkddnkiivttiqklnnlmkaesdlpvynqqvvfifdechrsqfgeaqknlkkkfkryyq
fgftgtpifpenalgsettasvfgrelhsyvitdairdekvlkfkvdyndvrpqfkslet
etdekklsaaenqqaflhpmriqeitqyilnnfrqkthrtfpgskgfnamlavssvdaak
ayyatfkrlqeeaanksatykplriatifsfaaneeqnaigeisdetfdtsamdssakef
ldaaireynshfktnfstdsngfqnyyrdlaqrvknqdidllivvgmfltgfdaptlntl
fvdknlryhglmqafsrtnriydatktfgnivtfrdlerstidaitlfgdkntknvvlek
syteymegftdaatgeakrgfmtvvseleqrfpdptsiesekekkdfvklfgeylraeni
lqnydefatlkalqqidlsdpvavekfkaehyvddekfaelqtirlpadrkiqdyrsayn
dirdwqrrekeaekkeksttdwddvvfevdllksq

    Sequence, based on observed residues (ATOM records):
    >2w74D (D:)
nfivldkyikaeptgdsyqsesdlereliqdlrnqgyefisvksqsamlanvreqlqnln
gvvfndsewrrfteqyldnpsdgildktrkihidyicdfifdderleniylidkknlmrn
kvqiiqqfrydvtilvnglplvqielkkrgvaireafnqinslfkylqlfvisngtdtry
fanttkrdrnsfdftmnwaksdntlikdlkdftatcfqkhtllnvlvnysvfdssqtllv
mrpyqiaaterilwkikssftaknwskpesggyiwhttgsgktltsfkaarlateldfid
kvffvvdrkdldyqtmkeyqrfspdsvngsentaglkrnldknkiivttiqklnnlmkae
sdlpvynqqvvfifdechrsqfgeaqknlkkkfkryyqfgftgtpifpenaettasvfgr
elhsyvitdairdekvlkfkvdyndvrpqfksletaflhpmriqeitqyilnnfrqkthr
kgfnamlavssvdaakayyatfplriatifsgeisdetfdtsakefldaaireynshfkt
nfstdsngfqnyyrdlaqrvknqdidllivvgmfltgfdaptlntlfvdknlryhglmqa
fsrtnriydatktfgnivtfrdlerstidaitlfgdkntknvvleksyteymegftdakr
gfmtvvseleqrfdfvklfgeylraenilqnydefatlkahyvddekfaelqtirlpadr
kiqdyrsayndivvfevdllks