PDB entry 2vum

View 2vum on RCSB PDB site
Description: Alpha-amanitin inhibited complete RNA polymerase II elongation complex
Class: transferase
Keywords: transferase, DNA-directed RNA polymerase, alpha-amanitin, ubl conjugation, nuclear protein, RNA polymerase II, elongation complex, mRNA processing, phosphorylation, enzyme inhibition, DNA damage, DNA repair, zinc-finger, DNA-binding, transferase/DNA/RNA, transcription bubble, zinc, nucleus, cytoplasm, inhibitor, magnesium, transcription inhibition, translocation intermediate, transcription, metal-binding, phosphoprotein, intermediate, fungal toxin, polymorphism, nucleotidyltransferase, transcription mechanism
Deposited on 2008-05-27, released 2008-06-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2018-06-27, with a file datestamp of 2018-06-22.
Experiment type: XRAY
Resolution: 3.4 Å
R-factor: N/A
AEROSPACI score: 0.08 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase II subunit RPB1
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vuma1
  • Chain 'B':
    Compound: DNA-directed RNA polymerase II subunit RPB2
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumb1
  • Chain 'C':
    Compound: DNA-directed RNA polymerase II subunit RPB3
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: DNA-directed RNA polymerase II subunit RPB4
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumd1
  • Chain 'E':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC1
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumf1
  • Chain 'G':
    Compound: DNA-directed RNA polymerase II subunit RPB7
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumg1
  • Chain 'H':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumh1
  • Chain 'I':
    Compound: DNA-directed RNA polymerase II subunit RPB9
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumj1
  • Chain 'K':
    Compound: DNA-directed RNA polymerase II subunit RPB11
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vumk1
  • Chain 'L':
    Compound: DNA-directed RNA polymerases I, II, and III subunit RPABC4
    Species: SACCHAROMYCES CEREVISIAE [TaxId:4932]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2vuml1
  • Chain 'M':
    Compound: amatoxin
    Species: AMANITA PHALLOIDES [TaxId:67723]
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: 5'-d(*ap*ap*ap*cp*tp*ap*cp*tp*tp*gp *ap*gp*cp*t)-3'
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'P':
    Compound: 5'-r(*ap*ap*ap*gp*ap*cp*cp*ap*gp*gp*c)-3'
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'T':
    Compound: 5'-d(*ap*gp*cp*tp*cp*ap*ap*gp*tp*ap *gp*tp*tp*ap*cp*gp*cp*cp*brup*gp*gp*tp*cp*ap*tp*t)-3'
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: ZN, MG

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2vumA (A:)
mvgqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgs
idrnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehne
lmrqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglk
lvgswkkdratgdadepelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclp
vppppvrpsisfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhv
atymdndiagqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnle
ldqvgvpksiaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskr
agdiqlqygwkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspyna
dfdgdemnlhvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtf
ieldqvlnmlywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllsp
kdngmliidgqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhn
gfstgigdtiadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvr
flneardkagrlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfv
drtlphfskddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrr
lvkaledimvhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvd
llntdhtldpsllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvni
rriiqnaqqtfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfcc
llrsrlatrrvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmt
lntfhfagvaskkvtsgvprlkeilnvaknmktpsltvylepghaadqeqaklirsaieh
ttlksvtiaseiyydpdprstvipedeeiiqlhfslldeeaeqsfdqqspwllrleldra
amndkdltmgqvgerikqtfkndlfviwsedndekliircrvvrpksldaeteaeedhml
kkientmlenitlrgveniervvmmkydrkvpsptgeyvkepewvletdgvnlsevmtvp
gidptriytnsfidimevlgieagraalykevynviasdgsyvnyrhmallvdvmttqgg
ltsvtrhgfnrsntgalmrcsfeetveilfeagasaelddcrgvsenvilgqmapigtga
fdvmideeslvkympeqkiteiedgqdggvtpysnesglvnadldvkdelmfsplvdsgs
ndamaggftayggadygeatspfgaygeaptspgfgvsspgfsptsptysptspayspts
psysptspsysptspsysptspsysptspsysptspsysptspsysptspsysptspsys
ptspsysptspsysptspsysptspsysptspsysptspaysptspsysptspsysptsp
sysptspsysptspnysptspsysptspgyspgspayspkqdeqkhnenensr

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumA (A:)
vgqqyssaplrtvkevqfglfspeevraisvakirfpetmdetqtrakigglndprlgsi
drnlkcqtcqegmnecpghfghidlakpvfhvgfiakikkvcecvcmhcgkllldehnel
mrqalaikdskkrfaaiwtlcktkmvcetdvpseddptqlvsrggcgntqptirkdglkl
vgswkdepelrvlsteeilnifkhisvkdftslgfnevfsrpewmiltclpvppppvrps
isfnesqrgeddltfkladilkanisletlehngaphhaieeaesllqfhvatymdndia
gqpqalqksgrpvksirarlkgkegrirgnlmgkrvdfsartvisgdpnleldqvgvpks
iaktltypevvtpynidrltqlvrngpnehpgakyvirdsgdridlryskragdiqlqyg
wkverhimdndpvlfnrqpslhkmsmmahrvkvipystfrlnlsvtspynadfdgdemnl
hvpqseetraelsqlcavplqivspqsnkpcmgivqdtlcgirkltlrdtfieldqvlnm
lywvpdwdgviptpaiikpkplwsgkqilsvaipngihlqrfdegttllspkdngmliid
gqiifgvvekktvgssngglihvvtrekgpqvcaklfgniqkvvnfwllhngfstgigdt
iadgptmreitetiaeakkkvldvtkeaqanlltakhgmtlresfednvvrflneardka
grlaevnlkdlnnvkqmvmagskgsfiniaqmsacvgqqsvegkriafgfvdrtlphfsk
ddyspeskgfvensylrgltpqefffhamggreglidtavktaetgyiqrrlvkaledim
vhydnttrnslgnviqfiygedgmdaahiekqsldtiggsdaafekryrvdllntdhtld
psllesgseilgdlklqvlldeeykqlvkdrkflrevfvdgeanwplpvnirriiqnaqq
tfhidhtkpsdltikdivlgvkdlqenllvlrgkneiiqnaqrdavtlfccllrsrlatr
rvlqeyrltkqafdwvlsnieaqflrsvvhpgemvgvlaaqsigepatqmtlntfhvtsg
vprlkeilnvaknmktpsltvylepghaadqeqaklirsaiehttlksvtiaseiyydpd
prstvipedeeiiqlhfslqqspwllrleldraamndkdltmgqvgerikqtfkndlfvi
wsedndekliircrvvaeedhmlkkientmlenitlrgveniervvmmkydrkvpsptge
yvkepewvletdgvnlsevmtvpgidptriytnsfidimevlgieagraalykevynvia
sdgsyvnyrhmallvdvmttqggltsvtrhgfnrsntgalmrcsfeetveilfeagasae
lddcrgvsenvilgqmapigtgafdvmideeslvkymp

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2vumB (B:)
msdlansekyydedpygfedesapitaedswavisaffrekglvsqqldsfnqfvdytlq
diicedstlileqlaqhttesdnisrkyeisfgkiyvtkpmvnesdgvthalypqearlr
nltyssglfvdvkkrtyeaidvpgrelkyeliaeeseddsesgkvfigrlpimlrskncy
lseatesdlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaei
rsalekgsrfistlqvklygregssartikatlpyikqdipiviifralgiipdgeileh
icydvndwqmlemlkpcvedgfviqdretaldfigrrgtalgikkekriqyakdilqkef
lphitqlegfesrkafflgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktl
fkkltkdifrymqrtveeahdfnmklainaktitsglkyalatgnwgeqkkamssragvs
qvlnrytysstlshlrrtntpigrdgklakprqlhnthwglvcpaetpegqacglvknls
lmscisvgtdpmpiitflsewgmepledyvphqspdatrvfvngvwhgvhrnparlmetl
rtlrrkgdinpevsmirdirekelkiftdagrvyrplfiveddeslghkelkvrkghiak
lmateyqdieggfedveeytwssllneglveyidaeeeesiliamqpedlepaeaneend
ldvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsprntyqsamgkqamgv
fltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaivaiacysgynqedsmi
mnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrmkhgtydkldddglia
pgvrvsgedviigkttpispdeeelgqrtayhskrdastplrstengivdqvlvttnqdg
lkfvkvrvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaipsr
mtvahliecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtgkk
lmaqiffgptyyqrlrhmvddkiharargpmqvltrqpvegrsrdgglrfgemerdcmia
hgaasflkerlmeasdafrvhicgicglmtviaklnhnqfeckgcdnkidiyqihipyaa
kllfqelmamnitprlytdrsrdf

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumB (B:)
desapitaedswavisaffrekglvsqqldsfnqfvdytlqdiicedstliisfgkiyvt
kpmvnesdgvthalypqearlrnltyssglfvdvkkkvfigrlpimlrskncylseates
dlyklkecpfdmggyfiingsekvliaqersagnivqvfkkaapspishvaeirsalekg
srfistlqvklygregssartikatlpyikqdipiviifralgiipdgeilehicydvnd
wqmlemlkpcvedgfviqdretaldfigkekriqyakdilqkeflphitqlegfesrkaf
flgyminrlllcaldrkdqddrdhfgkkrldlagpllaqlfktlfkkltkdifrymqrtv
elainaktitsglkyalatgnwgeqkkamssragvsqvlnrytysstlshlrrtntpiak
prqlhnthwglvcpaetpegqacglvknlslmscisvgtdpmpiitflsewgmepledyv
phqspdatrvfvngvwhgvhrnparlmetlrtlrrkgdinpevsmirdirekelkiftda
grvyrplfiveddeslghkelkvrkghiaklmateyqdeytwssllneglveyidaeeee
siliamqpedlepaeadvdpakrirvshhattfthceihpsmilgvaasiipfpdhnqsp
rntyqsamgkqamgvfltnynvrmdtmanilyypqkplgttrameylkfrelpagqnaiv
aiacysgynqedsmimnqssidrglfrslffrsymdqekkygmsitetfekpqrtntlrm
khgtydkldddgliapgvrvsgedviigkttpisskrdastplrstengivdqvlvttnq
dglkfvkvrvrttkipqigdkfasrhgqkgtigityrredmpftaegivpdliinphaip
srmtvahliecllskvaalsgnegdaspftditvegiskllrehgyqsrgfevmynghtg
kklmaqiffgptyyqrlrhmvddkiharargpmqvltrqpvegrsrdgglrfgemerdcm
iahgaasflkerlmeasdafrvhicgicglmtviaklnhnqfeckgcdnkidiyqihipy
aakllfqelmamnitprlytdrsrdf

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2vumD (D:)
mnvststfqtrrrrlkkveeeenaatlqlgqefqlkqinhqgeeeelialnlsearlvik
ealverrrafkrsqkkhkkkhlkhenandettavededddldeddvnaddddfmhsetre
kelesidvlleqttggnnkdlkntmqyltnfsrfrdqetvgaviqllkstglhpfevaql
gslacdtadeaktlipslnnkisddelerilkelsnletly

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumD (D:)
ststfqtrrrrlkkveeeenaatlqlgqefqlkqinhqgeeeelialnlsearlvikeal
verrrafkrsqkktrekelesidvlleqttggnnkdlkntmqyltnfsrfrdqetvgavi
qllkstglhpfevaqlgslacdtadeaktlipslnnkisddelerilkelsnletly

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2vumF (F:)
msdyeeafndgnenfedfdvehfsdeetyeekpqfkdgettdangktivtggngpedfqq
heqirrktlkekaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplri
amkelaekkiplvirrylpdgsfedwsveelivdl

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumF (F:)
kaipkdqrattpymtkyerarilgtralqismnapvfvdlegetdplriamkelaekkip
lvirrylpdgsfedwsveelivdl

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2vumG (G:)
mffikdlslnitlhpsffgprmkqylktklleevegsctgkfgyilcvldydnidiqrgr
ilptdgsaefnvkyravvfkpfkgevvdgtvvscsqhgfevqvgpmkvfvtkhlmpqdlt
fnagsnppsyqssedvitiksrirvkiegcisqvssihaigsikedylgai

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >2vumH (H:)
msntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtia
sslnledtpandssatrswrppqagdrsladdydyvmygtaykfeevskdliavyysfgg
llmrlegnyrnlnnlkqenayllirr

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumH (H:)
sntlfddifqvsevdpgrynkvcrieaasttqdqckltldinvelfpvaaqdsltvtias
sltrswrppqagdrsladdydyvmygtaykfeevskdliavyysfggllmrlegnyrnln
nlkqenayllirr

  • Chain 'I':
    No sequence available.

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >2vumJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynplekrd

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumJ (J:)
mivpvrcfscgkvvgdkwesylnllqedeldegtalsrlglkryccrrmilthvdliekf
lrynp

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >2vumK (K:)
mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtlaaddaf

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumK (K:)
mnapdrfelfllgegesklkidpdtkapnavvitfekedhtlgnliraellndrkvlfaa
ykvehpffarfklriqttegydpkdalknacnsiinklgalktnfetewnlqtl

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >2vumL (L:)
msregfqiptnldaaaagtsqartatlkyicaecssklslsrtdavrckdcghrillkar
tkrlvqfear

    Sequence, based on observed residues (ATOM records): (download) 
    >2vumL (L:)
atlkyicaecssklslsrtdavrckdcghrillkartkrlvqfear

  • Chain 'M':
    No sequence available.

  • Chain 'N':
    No sequence available.

  • Chain 'P':
    No sequence available.

  • Chain 'T':
    No sequence available.