PDB entry 2vot

View 2vot on RCSB PDB site
Description: structural and biochemical evidence for a boat-like transition state in beta-mannosidases
Class: hydrolase
Keywords: linear free energy relationship, hydrolase, transition state mimic, mannosidase, glycoside hydrolase
Deposited on 2008-02-20, released 2008-04-01
The last revision prior to the SCOP 1.75 freeze date was dated 2008-04-01, with a file datestamp of 2008-03-28.
Experiment type: XRAY
Resolution: 1.95 Å
R-factor: 0.16848
AEROSPACI score: 0.58 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-mannosidase
    Species: BACTEROIDES THETAIOTAOMICRON
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8AAK6 (Start-838)
    • PDB 2VOT
    Domains in SCOP 1.75: d2vota1, d2vota2, d2vota3, d2vota4, d2vota5
  • Chain 'B':
    Compound: beta-mannosidase
    Species: BACTEROIDES THETAIOTAOMICRON
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8AAK6 (Start-838)
    • PDB 2VOT (839-End)
    Domains in SCOP 1.75: d2votb1, d2votb2, d2votb3, d2votb4, d2votb5
  • Heterogens: BR, CL, NHV, EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2votA (A:)
qgndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwve
nedweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvl
rkgenhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmv
tsgvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvsln
gttvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqs
hriglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmk
eanmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavyni
rrlrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefds
drfyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsefgfqsfpemk
tiaafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgm
rhgleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiq
qndslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldg
wltpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlf
ssmlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretyke
hhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2votA (A:)
ndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwvene
dweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvlrk
genhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmvts
gvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvslngt
tvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqshr
iglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmkea
nmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavynirr
lrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefdsdr
fyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsefgfqsfpemkti
aafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgmrh
gleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiqqn
dslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldgwl
tpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlfss
mlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretyk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2votB (B:)
qgndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwve
nedweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvl
rkgenhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmv
tsgvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvsln
gttvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqs
hriglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmk
eanmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavyni
rrlrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefds
drfyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsefgfqsfpemk
tiaafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgm
rhgleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiq
qndslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldg
wltpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlf
ssmlakdifietplqgarysdnffdllpgerkkviitsprikkgeelpvnikhiretyke
hhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2votB (B:)
ndtsevmlldtgwefsqsgtekwmpatvpgtvhqdlishellpnpfygmnekkiqwvene
dweyrtsfivseeqlnrdgiqlifegldtyadvylngslllkadnmfvgytlpvksvlrk
genhlyiyfhspirqtlpqyasngfnypadndhhekhlsvfsrkapysygwdwgirmvts
gvwrpvtlrfydiatisdyyvrqlsltdenarlsnelivnqivpqkipaevrvnvslngt
tvtevkqqvtlqpginhitlpaevtnpvrwmpngwgtptlydfsaqiacgdrivaeqshr
iglrtirvvnekdkdgesfyfevngipmfakganyipqdallpnvtteryqtlfrdmkea
nmnmvriwgggtyennlfydladengilvwqdfmfactpypsdptflkrveaeavynirr
lrnhaslamwcgnneilealkywgfekkftpevyqglmhgydklfrellpstvkefdsdr
fyvhsspylanwgrpeswgtgdshnwgvwygkkpfesldtdlprfmsefgfqsfpemkti
aafaapedyqiesevmnahqkssignslirtymerdyiipesfedfvyvglvlqgqgmrh
gleahrrnrpycmgtlywqlndswpvvswssidyygnwkalhyqakrafapvlinpiqqn
dslsvylisdrldtmeqmtlemkvvdfdgktlgkkiqvhslevpantskcvyrakldgwl
tpedcrrsflklilkdksghqvaesvhffrktkdlqlpptsvsyqmkqtdgkceltlfss
mlakdifietplqgarysdnffdllpgerkkviitsprikkgelpvnikhiretykehh