PDB entry 2ven

View 2ven on RCSB PDB site
Description: structure-based enzyme engineering efforts with an inactive monomeric tim variant: the importance of a single point mutation for generating an active site with suitable binding properties
Class: isomerase
Keywords: isomerase, tim barrel, glycolysis, pentose shunt, gluconeogenesis, lipid synthesis, fatty acid biosynthesis, glycosome
Deposited on 2007-10-25, released 2008-02-19
The last revision prior to the SCOPe 2.01 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-31.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.188
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glycosomal triosephosphate isomerase
    Species: Trypanosoma brucei brucei [TaxId:5702]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04789 (0-237)
      • conflict (71-72)
      • conflict (90)
      • engineered mutation (223)
    Domains in SCOPe 2.01: d2vena_
  • Chain 'B':
    Compound: glycosomal triosephosphate isomerase
    Species: Trypanosoma brucei brucei [TaxId:5702]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04789 (0-237)
      • conflict (12)
      • conflict (71-72)
      • conflict (90)
      • engineered mutation (223)
    Domains in SCOPe 2.01: d2venb_
  • Heterogens: CIT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2venA (A:)
skpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfvi
aaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacige
tlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvatpqqaqeahal
irswvsskigadvagelrilyggsvngknartlyqqrdvngflaglkpefvdiikatq

    Sequence, based on observed residues (ATOM records): (download) 
    >2venA (A:)
skpqpiaaanwkslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfviaaqna
gnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacigetlqer
esgrtavvvltqiaaiakklkkadwakvviayepvwkvatpqqaqeahalirswvsskig
advagelrilyggsvngknartlyqqrdvngflaglkpefvdiikatq

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2venB (B:)
skpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfvi
aaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacige
tlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvatpqqaqeahal
irswvsskigadvagelrilyggsvngknartlyqqrdvngflaglkpefvdiikatq