PDB entry 2v9i

View 2v9i on RCSB PDB site
Description: l-rhamnulose-1-phosphate aldolase from escherichia coli (mutant e192a-k248w-l274stop)
Class: lyase
Keywords: entropy index, metal-binding, oligomerization, lyase, aldolase, class II, cleavage of l-rhamnulose-1-phosphate to dihydroxyacetoneph bacterial l-rhamnose metabolism, interface design, surface mutation, 2-ketose degradation, protein-protein interface, rare sugar, aggregation, zinc enzyme, fibrillation, rhamnose metabolism, protein engineering
Deposited on 2007-08-23, released 2008-01-15
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-31.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: 0.209
AEROSPACI score: 0.47 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: rhamnulose-1-phosphate aldolase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P32169 (Start-272)
      • engineered mutation (191)
      • engineered mutation (247)
    Domains in SCOPe 2.08: d2v9ia_
  • Chain 'B':
    Compound: rhamnulose-1-phosphate aldolase
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P32169 (Start-272)
      • engineered mutation (191)
      • engineered mutation (247)
    Domains in SCOPe 2.08: d2v9ib_
  • Heterogens: EDO, ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2v9iA (A:)
mqnitqswfvqgmikattdawlkgwdernggnltlrlddadiapyhdnfhqqpryiplsq
pmpllantpfivtgsgkffrnvqldpaanlgivkvdsdgagyhilwgltneavptselpa
hflshcerikatngkdrvimhchatnlialtyvlendtavftrqlwegsteclvvfpdgv
gilpwmvpgtdaigqataqemqkhslvlwpfhgvfgsgptldetfglidtaeksaqvlvk
vysmggmwqtisreelialgkrfgvtplasala

    Sequence, based on observed residues (ATOM records): (download) 
    >2v9iA (A:)
qnitqswfvqgmikattdawlkgwdernggnltlrlddadiapyhdnfhqqpryiplsqp
mpllantpfivtgsgkffrnvqldpaanlgivkvdsdgagyhilwgltneavptselpah
flshcerikatngkdrvimhchatnlialtyvlendtavftrqlwegsteclvvfpdgvg
ilpwmvpgtdaigqataqemqkhslvlwpfhgvfgsgptldetfglidtaeksaqvlvkv
ysmggmwqtisreelialgkrfgvtplasala

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2v9iB (B:)
mqnitqswfvqgmikattdawlkgwdernggnltlrlddadiapyhdnfhqqpryiplsq
pmpllantpfivtgsgkffrnvqldpaanlgivkvdsdgagyhilwgltneavptselpa
hflshcerikatngkdrvimhchatnlialtyvlendtavftrqlwegsteclvvfpdgv
gilpwmvpgtdaigqataqemqkhslvlwpfhgvfgsgptldetfglidtaeksaqvlvk
vysmggmwqtisreelialgkrfgvtplasala

    Sequence, based on observed residues (ATOM records): (download) 
    >2v9iB (B:)
qnitqswfvqgmikattdawlkgwdernggnltlrlddadiapyhdnfhqqpryiplsqp
mpllantpfivtgsgkffrnvqldpaanlgivkvdsdgagyhilwgltneavptselpah
flshcerikatngkdrvimhchatnlialtyvlendtavftrqlwegsteclvvfpdgvg
ilpwmvpgtdaigqataqemqkhslvlwpfhgvfgsgptldetfglidtaeksaqvlvkv
ysmggmwqtisreelialgkrfgvtplasala