PDB entry 2v7g

View 2v7g on RCSB PDB site
Description: Crystal Structure of an Engineered Urocanase Tetramer
Class: lyase
Keywords: histidine degradation, nad, lyase, protein engineering, urocanate hydratase, histidine metabolism
Deposited on 2007-07-30, released 2008-01-15
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-11-20, with a file datestamp of 2013-11-15.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.156
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Urocanate hydratase
    Species: Pseudomonas putida [TaxId:303]
    Database cross-references and differences (RAF-indexed):
    • PDB 2V7G
      • engineered mutation (197)
      • engineered mutation (276)
      • engineered mutation (290)
      • engineered mutation (300)
      • conflict (163-164)
      • conflict (166)
    • Uniprot P25080 (Start-556)
    Domains in SCOPe 2.08: d2v7ga_
  • Chain 'B':
    Compound: Urocanate hydratase
    Species: Pseudomonas putida [TaxId:303]
    Database cross-references and differences (RAF-indexed):
    • PDB 2V7G
      • engineered mutation (197)
      • engineered mutation (276)
      • engineered mutation (290)
      • engineered mutation (300)
      • conflict (163-164)
      • conflict (166)
    • Uniprot P25080 (Start-556)
    Domains in SCOPe 2.08: d2v7gb_
  • Chain 'C':
    Compound: Urocanate hydratase
    Species: Pseudomonas putida [TaxId:303]
    Database cross-references and differences (RAF-indexed):
    • PDB 2V7G
      • engineered mutation (197)
      • engineered mutation (276)
      • engineered mutation (290)
      • engineered mutation (300)
      • conflict (163-164)
      • conflict (166)
    • Uniprot P25080 (Start-556)
    Domains in SCOPe 2.08: d2v7gc_
  • Chain 'D':
    Compound: Urocanate hydratase
    Species: Pseudomonas putida [TaxId:303]
    Database cross-references and differences (RAF-indexed):
    • PDB 2V7G
      • engineered mutation (197)
      • engineered mutation (276)
      • engineered mutation (290)
      • engineered mutation (300)
      • conflict (163-164)
      • conflict (166)
    • Uniprot P25080 (Start-556)
    Domains in SCOPe 2.08: d2v7gd_
  • Heterogens: ACT, GOL, NAD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2v7gA (A:)
mtdnnkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraar
nwecydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfne
ldakglamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmgg
aqplaatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisial
hgnaaeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaak
asmavhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgp
frwaalsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglrak
lglafnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallnta
ggatwvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydi
aidcakeqgldlpmitg

    Sequence, based on observed residues (ATOM records): (download) 
    >2v7gA (A:)
nkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraarnwec
ydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfneldak
glamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmggaqpl
aatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisialhgna
aeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaakasma
vhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgpfrwa
alsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglraklgla
fnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallntaggat
wvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydiaidc
akeqgldlpmitg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2v7gB (B:)
mtdnnkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraar
nwecydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfne
ldakglamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmgg
aqplaatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisial
hgnaaeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaak
asmavhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgp
frwaalsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglrak
lglafnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallnta
ggatwvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydi
aidcakeqgldlpmitg

    Sequence, based on observed residues (ATOM records): (download) 
    >2v7gB (B:)
nkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraarnwec
ydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfneldak
glamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmggaqpl
aatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisialhgna
aeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaakasma
vhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgpfrwa
alsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglraklgla
fnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallntaggat
wvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydiaidc
akeqgldlpmitg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2v7gC (C:)
mtdnnkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraar
nwecydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfne
ldakglamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmgg
aqplaatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisial
hgnaaeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaak
asmavhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgp
frwaalsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglrak
lglafnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallnta
ggatwvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydi
aidcakeqgldlpmitg

    Sequence, based on observed residues (ATOM records): (download) 
    >2v7gC (C:)
nkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraarnwec
ydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfneldak
glamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmggaqpl
aatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisialhgna
aeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaakasma
vhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgpfrwa
alsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglraklgla
fnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallntaggat
wvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydiaidc
akeqgldlpmitg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2v7gD (D:)
mtdnnkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraar
nwecydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfne
ldakglamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmgg
aqplaatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisial
hgnaaeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaak
asmavhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgp
frwaalsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglrak
lglafnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallnta
ggatwvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydi
aidcakeqgldlpmitg

    Sequence, based on observed residues (ATOM records): (download) 
    >2v7gD (D:)
nkyrdveiraprgnkltakswlteaplrmlmnnldpqvaenpkelvvyggigraarnwec
ydkivetltrleddetllvqsgkpvgvfkthsnaprvliansnlvphwanwehfneldak
glamygqmtagswiyigsqgivqgtyetfveagrqhyggslkgkwvltaglggmggaqpl
aatlagacslniesqqsridfrletryvdeqatdlddalvriakytaegkaisialhgna
aeilpelvkrgvrpdmvtdqtsahdplngylpigwtweqyrdraqtipaavvkaakasma
vhvqamldfqkqgvptfdygnnirqmakeegvanafdfpgfvpayirplfcrgvgpfrwa
alsgeaediyktdakvkelipddahlhrwldmarerisfqglparicwvglglraklgla
fnemvrsgelsapvvigrdhldsgsvsspnreteamrdgsdavsdwpllnallntaggat
wvslhhgggvgmgfsqhsgmvivcdgtdeaaeriarvltndpgtgvmrhadagydiaidc
akeqgldlpmitg