PDB entry 2v2h

View 2v2h on RCSB PDB site
Description: The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Class: isomerase
Keywords: gluconeogenesis, lipid synthesis, 2-phospho glycolate, glycolysis, engineering, pentose shunt, point mutation, tim, 2pg, a178l, loop6, hinge, loop-6, enzyme, fatty acid biosynthesis, triosephosphate isomerase, isomerase, glycosome, monomeric, tim-barrel
Deposited on 2007-06-06, released 2008-02-19
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-07-24, with a file datestamp of 2019-07-19.
Experiment type: XRAY
Resolution: 1.18 Å
R-factor: N/A
AEROSPACI score: 0.66 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: triosephosphate isomerase glycosomal
    Species: Trypanosoma brucei brucei [TaxId:5702]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04789 (71-241)
      • conflict (13)
      • conflict (16-17)
      • conflict (66-70)
      • conflict (72-73)
      • conflict (91)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d2v2ha_
  • Chain 'B':
    Compound: triosephosphate isomerase glycosomal
    Species: Trypanosoma brucei brucei [TaxId:5702]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04789 (71-241)
      • conflict (13)
      • conflict (16-17)
      • conflict (66-70)
      • conflict (72-73)
      • conflict (91)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d2v2hb_
  • Chain 'C':
    Compound: triosephosphate isomerase glycosomal
    Species: Trypanosoma brucei brucei [TaxId:5702]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04789 (71-241)
      • conflict (13)
      • conflict (16-17)
      • conflict (66-70)
      • conflict (72-73)
      • conflict (91)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d2v2hc_
  • Heterogens: PGA, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2v2hA (A:)
mskpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfv
iaaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacig
etlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeaha
lirswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiika
tq

    Sequence, based on observed residues (ATOM records): (download) 
    >2v2hA (A:)
skpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfvi
aaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacige
tlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeahal
irswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiikat
q

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2v2hB (B:)
mskpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfv
iaaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacig
etlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeaha
lirswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiika
tq

    Sequence, based on observed residues (ATOM records): (download) 
    >2v2hB (B:)
skpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfvi
aaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacige
tlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeahal
irswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiikat
q

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2v2hC (C:)
mskpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfv
iaaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacig
etlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeaha
lirswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiika
tq

    Sequence, based on observed residues (ATOM records): (download) 
    >2v2hC (C:)
skpqpiaaanwksgspdslselidlfnstsinhdvqcvvastfvhlamtkerlshpkfvi
aaqnagnadalaslkdfgvnwivlghserrwyygetneivadkvaaavasgfmviacige
tlqeresgrtavvvltqiaaiakklkkadwakvviayepvwaigtgkvltpqqaqeahal
irswvsskigadvagelrilyggsvngknartlyqqrdvngflvggaslkpefvdiikat
q