PDB entry 2rhs

View 2rhs on RCSB PDB site
Description: PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies
Class: ligase
Keywords: heterotetramer, phenylalanine, tRNA, Aminoacyl-tRNA synthetase, ATP-binding, Cytoplasm, Ligase, Magnesium, Metal-binding, Nucleotide-binding, Protein biosynthesis, RNA-binding, tRNA-binding
Deposited on 2007-10-09, released 2007-11-06
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: phenylalanyl-tRNA synthetase alpha chain
    Species: Staphylococcus haemolyticus [TaxId:1283]
    Gene: pheS
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d2rhsa_
  • Chain 'B':
    Compound: phenylalanyl-tRNA synthetase beta chain
    Species: Staphylococcus haemolyticus [TaxId:1283]
    Gene: pheT
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4L5E4 (0-799)
      • engineered (33)
      • engineered (143)
      • engineered (660)
      • engineered (662-663)
  • Chain 'C':
    Compound: phenylalanyl-tRNA synthetase alpha chain
    Species: Staphylococcus haemolyticus [TaxId:1283]
    Gene: pheS
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4L5E3 (26-293)
      • expression tag (23-25)
    Domains in SCOPe 2.08: d2rhsc1, d2rhsc2
  • Chain 'D':
    Compound: phenylalanyl-tRNA synthetase beta chain
    Species: Staphylococcus haemolyticus [TaxId:1283]
    Gene: pheT
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q4L5E4 (0-End)
      • engineered (33)
      • engineered (143)
      • engineered (660)
      • engineered (662-663)
  • Heterogens: GAX, SO4, ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2rhsA (A:)
mgsshhhhhhssglvprgshmgtelmeklnqqlaeetidvtlpsrqisigskhpltrtve
eiedlflglgyeivdgyeveqdyynfealnlpkshpardmqdsfyitdeilmrthtspvq
artmekrngqgpvkiicpgkvyrrdsddathshqftqieglvvdknikmsdlkgtlelva
kklfgadreirlrpsyfpftepsvevdvscfkckgkgcnvckhtgwieilgagmvhpnvl
emagfdsneysgfafgmgpdriamlkygiediryfytndvrfleqfkavedrge

    Sequence, based on observed residues (ATOM records): (download) 
    >2rhsA (A:)
nqqlaeetidvtlpsrqisigskhpltrtveeiedlflglgyeivdgyeveqdyynfeal
nlpkshpardmqdsfyitdeilmrthtspvqartmekrngqgpvkiicpgkvyrrdsdda
thshqftqieglvvdknikmsdlkgtlelvakklfgadreirlrpsyfpftepsvevdvs
wieilgagmvhpnvlemagfdsneysgfafgmgpdriamlkygiediryfytndvrfleq
fkavedrge

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2rhsC (C:)
mgsshhhhhhssglvprgshmgtelmeklnqqlaeetidvtlpsrqisigskhpltrtve
eiedlflglgyeivdgyeveqdyynfealnlpkshpardmqdsfyitdeilmrthtspvq
artmekrngqgpvkiicpgkvyrrdsddathshqftqieglvvdknikmsdlkgtlelva
kklfgadreirlrpsyfpftepsvevdvscfkckgkgcnvckhtgwieilgagmvhpnvl
emagfdsneysgfafgmgpdriamlkygiediryfytndvrfleqfkavedrge

    Sequence, based on observed residues (ATOM records): (download) 
    >2rhsC (C:)
elmeklnqqlaeetidvtlpsrqisigskhpltrtveeiedlflglgyeivdgyeveqdy
ynfealnlpkshpardmqdsfyitdeilmrthtspvqartmekrngqgpvkiicpgkvyr
rdsddathshqftqieglvvdknikmsdlkgtlelvakklfgadreirlrpsyfpfteps
vevdvscfkckgkgcnvckhtgwieilgagmvhpnvlemagfdsneysgfafgmgpdria
mlkygiediryfytndvrfleqfkavedrge

  • Chain 'D':
    No sequence available.