PDB entry 2ns7

View 2ns7 on RCSB PDB site
Description: How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor
Class: transcription
Keywords: transcription regulator, helix-turn-helix, Transcription
Deposited on 2006-11-03, released 2007-07-24
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-18, with a file datestamp of 2017-10-13.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: N/A
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Tetracycline repressor protein
    Species: Escherichia coli [TaxId:83333]
    Gene: tetR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04483 (Start-186)
      • engineered (67)
      • engineered (87)
      • engineered (120)
      • engineered (143)
    • Uniprot P0ACT4 (187-End)
    Domains in SCOPe 2.08: d2ns7a1, d2ns7a2
  • Chain 'B':
    Compound: Tetracycline repressor protein
    Species: Escherichia coli [TaxId:83333]
    Gene: tetR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04483 (Start-186)
      • engineered (67)
      • engineered (87)
      • engineered (120)
      • engineered (143)
    • Uniprot P0ACT4 (187-End)
    Domains in SCOPe 2.08: d2ns7b1, d2ns7b2
  • Chain 'C':
    Compound: Tetracycline repressor protein
    Species: Escherichia coli [TaxId:83333]
    Gene: tetR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04483 (Start-186)
      • engineered (67)
      • engineered (87)
      • engineered (120)
      • engineered (143)
    • Uniprot P0ACT4 (187-End)
    Domains in SCOPe 2.08: d2ns7c1, d2ns7c2
  • Chain 'D':
    Compound: Tetracycline repressor protein
    Species: Escherichia coli [TaxId:83333]
    Gene: tetR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P04483 (Start-186)
      • engineered (67)
      • engineered (87)
      • engineered (120)
      • engineered (143)
    • Uniprot P0ACT4 (187-End)
    Domains in SCOPe 2.08: d2ns7d1, d2ns7d2

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2ns7A (A:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaieml
drhhthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafl
tqqgfslenalyalsavghftlgsvledqehqvakeeretpttdsmppllrqaielfdhq
gaepaflhgleslirgfevqltallqiv

    Sequence, based on observed residues (ATOM records): (download) 
    >2ns7A (A:)
dkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaiemldrhh
thfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafltqqg
fslenalyalsavghftlgsvledqehqvakttdsmppllrqaielfdhqgaepaflhgl
eslirgfevqltallq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2ns7B (B:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaieml
drhhthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafl
tqqgfslenalyalsavghftlgsvledqehqvakeeretpttdsmppllrqaielfdhq
gaepaflhgleslirgfevqltallqiv

    Sequence, based on observed residues (ATOM records): (download) 
    >2ns7B (B:)
rldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaiemldr
hhthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafltq
qgfslenalyalsavghftlgsvledqehqvakdsmppllrqaielfdhqgaepaflhgl
eslirgfevqltal

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2ns7C (C:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaieml
drhhthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafl
tqqgfslenalyalsavghftlgsvledqehqvakeeretpttdsmppllrqaielfdhq
gaepaflhgleslirgfevqltallqiv

    Sequence, based on observed residues (ATOM records): (download) 
    >2ns7C (C:)
skvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaiemldrhhth
fsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafltqqgfs
lenalyalsavghftlgsvledqehqvakttdsmppllrqaielfdhqgaepaflhgles
lirgfevqlt

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2ns7D (D:)
msrldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaieml
drhhthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafl
tqqgfslenalyalsavghftlgsvledqehqvakeeretpttdsmppllrqaielfdhq
gaepaflhgleslirgfevqltallqiv

    Sequence, based on observed residues (ATOM records): (download) 
    >2ns7D (D:)
ldkskvinsalellnevgieglttrklaqklgveqptlywhvknkralldalaiemldrh
hthfsplegeswqdflrnnaksfrnallshrdgakvhlgtrptekqyetlenqlafltqq
gfslenalyalsavghftlgsvledqehqvakegaepaflhgleslirgfevqltall