PDB entry 2nlz

View 2nlz on RCSB PDB site
Description: Crystal structure of cephalosporin acylase from Bacillus halodurans
Class: structural genomics, unknown function
Keywords: ACYLASE, STRUCTURAL GENOMICS, PROTEIN STRUCTURE INITIATIVE, PSI, NYSGXRC, New York Structural GenomiX Research Consortium STRUCTURAL GENOMICS
Deposited on 2006-10-20, released 2006-11-07
The last revision prior to the SCOP 1.75 freeze date was dated 2006-11-07, with a file datestamp of 2007-06-04.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: 0.211
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: cephalosporin acylase
    Species: Bacillus halodurans
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KEI5 (Start-538)
      • engineered (169)
      • cloning artifact (539-540)
      • his tag (541)
    Domains in SCOP 1.75: d2nlza1
  • Chain 'B':
    Compound: cephalosporin acylase
    Species: Bacillus halodurans
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KEI5 (Start-538)
      • engineered (169)
      • cloning artifact (539-540)
      • his tag (541)
    Domains in SCOP 1.75: d2nlzb1
  • Chain 'C':
    Compound: cephalosporin acylase
    Species: Bacillus halodurans
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KEI5 (Start-538)
      • engineered (169)
      • cloning artifact (539-540)
      • his tag (541)
    Domains in SCOP 1.75: d2nlzc1
  • Chain 'D':
    Compound: cephalosporin acylase
    Species: Bacillus halodurans
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9KEI5 (Start-538)
      • engineered (169)
      • cloning artifact (539-540)
      • his tag (541-543)
    Domains in SCOP 1.75: d2nlzd1
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2nlzA (A:)
msvmfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltv
leptsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvp
gapgawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyq
pwfdtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggylt
kedlacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkq
ieamklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtpprggtvy
latadgdgnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyht
iipgfltkndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvq
veptfpvdiaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawe
ghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2nlzA (A:)
vmfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltvle
ptsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvpga
pgawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyqpw
fdtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggyltke
dlacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkqie
amklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtptvylatadg
dgnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyhtiipgfl
tkndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvqveptfp
vdiaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawegh

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2nlzB (B:)
msvmfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltv
leptsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvp
gapgawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyq
pwfdtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggylt
kedlacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkq
ieamklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtpprggtvy
latadgdgnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyht
iipgfltkndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvq
veptfpvdiaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawe
ghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2nlzB (B:)
mfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltvlep
tsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvpgap
gawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyqpwf
dtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggyltked
lacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkqiea
mklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtptvylatadgd
gnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyhtiipgflt
kndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvqveptfpv
diaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawegh

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2nlzC (C:)
msvmfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltv
leptsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvp
gapgawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyq
pwfdtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggylt
kedlacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkq
ieamklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtpprggtvy
latadgdgnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyht
iipgfltkndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvq
veptfpvdiaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawe
ghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2nlzC (C:)
mfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltvlep
tsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvpgap
gawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyqpwf
dtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggyltked
lacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkqiea
mklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtptvylatadgd
gnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyhtiipgflt
kndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvqveptfpv
diaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawegh

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2nlzD (D:)
msvmfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltv
leptsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvp
gapgawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyq
pwfdtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggylt
kedlacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkq
ieamklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtpprggtvy
latadgdgnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyht
iipgfltkndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvq
veptfpvdiaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaawe
ghhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >2nlzD (D:)
mfdpqsypypsrrnvvyakngmvatsqplaaqagldilkaggnaidaaiatataltvlep
tsngigsdafalvwtkgklhglngsgrapmsltmeavkakgyeqelppygvipvtvpgap
gawaelakmygnlplaaslapairyaeegypvtptlakywkaaydrvktewtddvyqpwf
dtfapkgraprvgevwrsqghadtlrsiaesngesfyrgeladqihaffdkhggyltked
lacyrpewvepisidyrgyrvweippngqglvalealnivkgfefyhkdtvdtyhkqiea
mklafvdgmkyvtepsdmsvsveqllsdeyaterrkeigeqaltpepgtptvylatadgd
gnmvsfiqsnymgfgsgvvvpgtgiamqnrghnfsldpnhdnalkpgkrtyhtiipgflt
kndqpigpfgvmggfmqpqghmqvmmntidfglnpqaaldaprwqwtngkqvqveptfpv
diaqalvrrghkiqvvldegafgrgqiiwrdpttgvlaggteprtdgqvaaweghhh