PDB entry 2j2f

View 2j2f on RCSB PDB site
Description: the t199d mutant of stearoyl acyl carrier protein desaturase from ricinus communis (castor bean)
Class: oxidoreductase
Keywords: electron transfer, four-helix bundle, oxidoreductase, lipid synthesis, transit peptide, fatty acid biosynthesis, nadp, chloroplast, di-ron enzyme
Deposited on 2006-08-16, released 2006-10-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-31.
Experiment type: XRAY
Resolution: 2.65 Å
R-factor: 0.242
AEROSPACI score: 0.25 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2fa_
  • Chain 'B':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2fb_
  • Chain 'C':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2fc_
  • Chain 'D':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2fd_
  • Chain 'E':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2fe_
  • Chain 'F':
    Compound: acyl-[acyl-carrier-protein] desaturase
    Species: Ricinus communis [TaxId:3988]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22337 (Start-362)
      • engineered mutation (198)
    Domains in SCOPe 2.08: d2j2ff_
  • Heterogens: FE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2j2fA (A:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fA (A:)
kpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdg
fdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiw
trawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfq
eradfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlaf
admmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltg
lsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2j2fB (B:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fB (B:)
pfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdgf
deqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiwt
rawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfqe
radfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlafa
dmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltgl
saegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2j2fC (C:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fC (C:)
fmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdgfd
eqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiwtr
awtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfqer
adfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlafad
mmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltgls
aegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2j2fD (D:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fD (D:)
pfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdgf
deqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiwt
rawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfqe
radfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlafa
dmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltgl
saegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2j2fE (E:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fE (E:)
pfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdgf
deqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiwt
rawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfqe
radfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlafa
dmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltgl
saegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2j2fF (F:)
astlksgskevenlkkpfmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvek
cwqpqdflpdpasdgfdeqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldg
vrdetgasptswaiwtrawtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdpr
tenspylgfiytsfqeradfishgntarqakehgdiklaqicgtiaadekrhetaytkiv
eklfeidpdgtvlafadmmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadi
leflvgrwkvdkltglsaegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrq
vkl

    Sequence, based on observed residues (ATOM records): (download) 
    >2j2fF (F:)
fmpprevhvqvthsmppqkieifksldnwaeenilvhlkpvekcwqpqdflpdpasdgfd
eqvrelrerakeipddyfvvlvgdmiteealptyqtmlntldgvrdetgasptswaiwtr
awtaeenrhgdllnkylylsgrvdmrqiektiqyligsgmdprtenspylgfiytsfqer
adfishgntarqakehgdiklaqicgtiaadekrhetaytkiveklfeidpdgtvlafad
mmrkkismpahlmydgrddnlfdhfsavaqrlgvytakdyadileflvgrwkvdkltgls
aegqkaqdyvcrlpprirrleeraqgrakeaptmpfswifdrqvkl