PDB entry 2iw4

View 2iw4 on RCSB PDB site
Description: crystal structure of basillus subtilis family II inorganic pyrophosphatase mutant, h98q, in complex with pnp
Class: hydrolase
Keywords: hydrolase, pyrophosphatase, substrate complex, mutant, manganese, metal-binding
Deposited on 2006-06-26, released 2006-11-07
The last revision prior to the SCOPe 2.07 freeze date was dated 2011-07-13, with a file datestamp of 2011-05-31.
Experiment type: XRAY
Resolution: 2.15 Å
R-factor: 0.17
AEROSPACI score: 0.42 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Manganese-dependent inorganic pyrophosphatase
    Species: Bacillus subtilis [TaxId:1423]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37487 (Start-308)
      • engineered mutation (97)
    Domains in SCOPe 2.07: d2iw4a_
  • Chain 'B':
    Compound: Manganese-dependent inorganic pyrophosphatase
    Species: Bacillus subtilis [TaxId:1423]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37487 (0-308)
      • engineered mutation (97)
    Domains in SCOPe 2.07: d2iw4b_
  • Heterogens: FE, MG, MN, 2PN, SO4, PG4, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2iw4A (A:)
mekilifghqnpdtdticsaiayadlknklgfnaepvrlgqvngetqyaldyfkqesprl
vetaanevngvilvdhnerqqsikdieevqvlevidhqrianfetaeplyyraepvgcta
tilnkmykennvkiekeiaglmlsaiisdsllfksptctdqdvaaakelaeiagvdaeey
glnmlkagadlskktveelisldakeftlgskkveiaqvntvdiedvkkrqaeleavisk
vvaeknldlfllvitdilendslalaigneaakvekafnvtlenntallkgvvsrkkqvv
pvltdamae

    Sequence, based on observed residues (ATOM records): (download) 
    >2iw4A (A:)
ekilifghqnpdtdticsaiayadlknklgfnaepvrlgqvngetqyaldyfkqesprlv
etaanevngvilvdhnerqqsikdieevqvlevidhqrianfetaeplyyraepvgctat
ilnkmykennvkiekeiaglmlsaiisdsllfksptctdqdvaaakelaeiagvdaeeyg
lnmlkagadlskktveelisldakeftlgskkveiaqvntvdiedvkkrqaeleaviskv
vaeknldlfllvitdilendslalaigneaakvekafnvtlenntallkgvvsrkkqvvp
vltdamae

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >2iw4B (B:)
mekilifghqnpdtdticsaiayadlknklgfnaepvrlgqvngetqyaldyfkqesprl
vetaanevngvilvdhnerqqsikdieevqvlevidhqrianfetaeplyyraepvgcta
tilnkmykennvkiekeiaglmlsaiisdsllfksptctdqdvaaakelaeiagvdaeey
glnmlkagadlskktveelisldakeftlgskkveiaqvntvdiedvkkrqaeleavisk
vvaeknldlfllvitdilendslalaigneaakvekafnvtlenntallkgvvsrkkqvv
pvltdamae