PDB entry 2ign

View 2ign on RCSB PDB site
Description: Crystal structure of recombinant pyranose 2-oxidase H167A mutant
Class: oxidoreductase
Keywords: oxidoreductase, gmc oxidoreductase, h167a mutant, rossmann fold, phbh fold, homotetramer, 8-alpha-(n3) histidyl flavinylation
Deposited on 2006-09-22, released 2006-10-10
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-10-18, with a file datestamp of 2017-10-13.
Experiment type: XRAY
Resolution: 1.65 Å
R-factor: N/A
AEROSPACI score: 0.42 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2igna1, d2igna2
  • Chain 'B':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2ignb1, d2ignb2
  • Chain 'C':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2ignc1, d2ignc2
  • Chain 'D':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2ignd1, d2ignd2
  • Chain 'E':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2igne1, d2igne2
  • Chain 'F':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2ignf1, d2ignf2
  • Chain 'G':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2igng1, d2igng2
  • Chain 'H':
    Compound: Pyranose oxidase
    Species: Trametes ochracea [TaxId:230624]
    Gene: p2o
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7ZA32
      • engineered (166)
    Domains in SCOPe 2.08: d2ignh1, d2ignh2
  • Heterogens: FAD, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2ignA (A:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignA (A:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2ignB (B:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignB (B:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >2ignC (C:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignC (C:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >2ignD (D:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignD (D:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >2ignE (E:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignE (E:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >2ignF (F:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignF (F:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >2ignG (G:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignG (G:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >2ignH (H:)
mstsssdpffnfakssfrsaaaqkasasslpplpgpdkkvpgmdikydvvivgsgpigct
yarelvgagykvamfdigeidsglkigahkkntveyqknidkfvnviqgqlmsvsvpvnt
lvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvggmstawtcatprfdreqr
pllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlvlnklteeykgqrdfqqip
laatrrsptfvewssantvfdlqnrpntdapeerfnlfpavacervvrnalnseieslhi
hdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnpanppellpslgsyiteqs
lvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastnkhpdwwnekvknhmmqhq
edplpipfedpepqvttlfqpshpwhtqihrdafsygavqqsidsrlivdwrffgrtepk
eenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdmcvmsakiggflpgslpqf
mepglvlhlggthrmgfdekednccvntdsrvfgfknlflggcgniptayganptltams
laiksceyikqnftpspftseaq

    Sequence, based on observed residues (ATOM records): (download) 
    >2ignH (H:)
mdikydvvivgsgpigctyarelvgagykvamfdigeidsglkigahkkntveyqknidk
fvnviqgqlmsvsvpvntlvvdtlsptswqastffvrngsnpeqdplrnlsgqavtrvvg
gmstawtcatprfdreqrpllvkddadaddaewdrlytkaesyfqtgtdqfkesirhnlv
lnklteeykgqrdfqqiplaatrrsptfvewssantvfdlqnrpntdapeerfnlfpava
cervvrnalnseieslhihdlisgdrfeikadvyvltagavhntqllvnsgfgqlgrpnp
anppellpslgsyiteqslvfcqtvmstelidsvksdmtirgtpgeltysvtytpgastn
khpdwwnekvknhmmqhqedplpipfedpepqvttlfqpshpwhtqihrdafsygavqqs
idsrlivdwrffgrtepkeenklwfsdkitdaynmpqptfdfrfpagrtskeaedmmtdm
cvmsakiggflpgslpqfmepglvlhlggthrmgfdekednccvntdsrvfgfknlflgg
cgniptayganptltamslaiksceyikqnftpspft