PDB entry 2i3p

View 2i3p on RCSB PDB site
Description: K28R mutant of Homing Endonuclease I-CreI
Class: hydrolase/DNA
Keywords: Homing endonulease I-CreI, DNA, HYDROLASE/DNA COMPLEX
Deposited on 2006-08-20, released 2006-09-05
The last revision prior to the SCOPe 2.05 freeze date was dated 2009-02-24, with a file datestamp of 2009-03-01.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.234
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725
      • engineered (27)
      • engineered (41)
      • engineered (109-110)
    Domains in SCOPe 2.05: d2i3pa_
  • Chain 'B':
    Compound: DNA endonuclease I-crei
    Species: Chlamydomonas reinhardtii [TaxId:3055]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05725 (Start-152)
      • engineered (27)
      • engineered (41)
      • engineered (109-110)
    Domains in SCOPe 2.05: d2i3pb_
  • Chain 'C':
    Compound: 5'-d(*gp*cp*ap*ap*ap*tp*cp*gp*tp*cp*gp*tp*gp*ap*gp*ap*cp*ap*ap*tp*tp*tp*cp*g)-3'
  • Chain 'D':
    Compound: 5'-d(*cp*gp*ap*ap*ap*tp*tp*gp*tp*cp*tp*cp*ap*cp*gp*ap*cp*gp*ap*tp*tp*tp*gp*c)-3'
  • Heterogens: CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >2i3pA (A:)
mntkynkefllylagfvdgdgsiiaqirpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravld

    Sequence, based on observed residues (ATOM records): (download) 
    >2i3pA (A:)
ntkynkefllylagfvdgdgsiiaqirpnqsykfkhqlsltfqvtqktqrrwfldklvde
igvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdk
flevctwvdqiaalndsktrkttsetvravld

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >2i3pB (B:)
mntkynkefllylagfvdgdgsiiaqirpnqsykfkhqlsltfqvtqktqrrwfldklvd
eigvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespd
kflevctwvdqiaalndsktrkttsetvravld

    Sequence, based on observed residues (ATOM records): (download) 
    >2i3pB (B:)
ntkynkefllylagfvdgdgsiiaqirpnqsykfkhqlsltfqvtqktqrrwfldklvde
igvgyvrdrgsvsdyilseikplhnfltqlqpflklkqkqanlvlkiieqlpsakespdk
flevctwvdqiaalndsktrkttsetvravld

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.