PDB entry 2i2t

View 2i2t on RCSB PDB site
Description: Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 50s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.
Class: ribosome
Keywords: ribosome, protein-nucleic acid complexes
Deposited on 2014-12-05, released 2006-10-24
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-12-10, with a file datestamp of 2014-12-05.
Experiment type: XRAY
Resolution: 3.22 Å
R-factor: 0.287
AEROSPACI score: 0.04 (click here for full SPACI score report)

Chains and heterogens:

  • Chain '0':
    Compound: 50S ribosomal protein L32
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2t01
  • Chain '1':
    Compound: 50S ribosomal protein L33
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2t11
  • Chain '2':
    Compound: 50S ribosomal protein L34
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2t21
  • Chain '3':
    Compound: 50S ribosomal protein L35
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2t31
  • Chain '4':
    Compound: 50S ribosomal protein L36
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2t41
  • Chain 'A':
    Compound: 5S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'B':
    Compound: 23S ribosomal RNA
    Species: Escherichia coli [TaxId:562]
  • Chain 'C':
    Compound: 50S ribosomal protein L2
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tc1, d2i2tc2
  • Chain 'D':
    Compound: 50S ribosomal protein L3
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2td1
  • Chain 'E':
    Compound: 50S ribosomal protein L4
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2te1
  • Chain 'F':
    Compound: 50S ribosomal protein L5
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tf1
  • Chain 'G':
    Compound: 50S ribosomal protein L6
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tg1, d2i2tg2
  • Chain 'H':
    Compound: 50S ribosomal protein L9
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2th1, d2i2th2
  • Chain 'I':
    Compound: 50S ribosomal protein L11
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2ti1, d2i2ti2
  • Chain 'J':
    Compound: 50S ribosomal protein L13
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tj1
  • Chain 'K':
    Compound: 50S ribosomal protein L14
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tk1
  • Chain 'L':
    Compound: 50S ribosomal protein L15
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tl1
  • Chain 'M':
    Compound: 50S ribosomal protein L16
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tm1
  • Chain 'N':
    Compound: 50S ribosomal protein L17
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tn1
  • Chain 'O':
    Compound: 50S ribosomal protein L18
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2to1
  • Chain 'P':
    Compound: 50S ribosomal protein L19
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tp1
  • Chain 'Q':
    Compound: 50S ribosomal protein L20
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tq1
  • Chain 'R':
    Compound: 50S ribosomal protein L21
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tr1
  • Chain 'S':
    Compound: 50S ribosomal protein L22
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2ts1
  • Chain 'T':
    Compound: 50S ribosomal protein L23
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tt1
  • Chain 'U':
    Compound: 50S ribosomal protein L24
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tu1
  • Chain 'V':
    Compound: 50S ribosomal protein L25
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tv1
  • Chain 'W':
    Compound: 50S ribosomal protein L27
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tw1
  • Chain 'X':
    Compound: 50S ribosomal protein L28
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tx1
  • Chain 'Y':
    Compound: 50S ribosomal protein L29
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2ty1
  • Chain 'Z':
    Compound: 50S ribosomal protein L30
    Species: Escherichia coli [TaxId:562]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.06: d2i2tz1
  • Heterogens: ZN, MG, HOH

PDB Chain Sequences:

  • Chain '0':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2t0 (0:)
    avqqnkptrskrgmrrshdaltavtslsvdktsgekhlrhhitadgyyrgrkviak
    

  • Chain '1':
    Sequence, based on SEQRES records: (download)
    >2i2t1 (1:)
    akgirekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeakik
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2t1 (1:)
    girekiklvssagtghfytttknkrtkpeklelkkfdpvvrqhviykeak
    

  • Chain '2':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2t2 (2:)
    mkrtfqpsvlkrnrshgfrarmatkngrqvlarrrakgrarltvsk
    

  • Chain '3':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2t3 (3:)
    pkiktvrgaakrfkktgkggfkhkhanlrhiltkkatkrkrhlrpkamvskgdlglviac
    lpya
    

  • Chain '4':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2t4 (4:)
    mkvrasvkklcrnckivkrdgvirvicsaepkhkqrqg
    

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >2i2tC (C:)
    avvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkqa
    yrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgvd
    aaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsgem
    rkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnfg
    khpvtpwgvqtkgkktrsnkrtdkfivrrrsk
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tC (C:)
    avvkckptspgrrhvvkvvnpelhkgkpfaplleknsksggrnnngrittrhiggghkqa
    yrivdfkrnkdgipavverleydpnrsanialvlykdgerryilapkglkagdqiqsgvd
    aaikpgntlpmrnipvgstvhnvemkpgkggqlarsagtyvqivardgayvtlrlrsgem
    rkveadcratlgevgnaehmlrvlgkagaarwrgvrptvrgtamnpvdhphgggegrnfg
    khpvtpwgvqtkgkktrsnkrtdkfivrrrs
    

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tD (D:)
    miglvgkkvgmtriftedgvsipvtvieveanrvtqvkdlandgyraiqvttgakkanrv
    tkpeaghfakagveagrglwefrlaegeeftvgqsisvelfadvkkvdvtgtskgkgfag
    tvkrwnfrtqdathgnslshrvpgsigqnqtpgkvfkgkkmagqmgnervtvqsldvvrv
    daernlllvkgavpgatgsdlivkpavka
    

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tE (E:)
    melvlkdaqsaltvsettfgrdfnealvhqvvvayaagarqgtraqktraevtgsgkkpw
    rqkgtgrarsgsikspiwrsggvtfaarpqdhsqkvnkkmyrgalksilselvrqdrliv
    vekfsveapktkllaqklkdmaledvliitgeldenlflaarnlhkvdvrdatgidpvsl
    iafdkvvmtadavkqveemla
    

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tF (F:)
    aklhdyykdevvkklmtefnynsvmqvprvekitlnmgvgeaiadkklldnaaadlaais
    gqkplitkarksvagfkirqgypigckvtlrgermwefferlitiavprirdfrglsaks
    fdgrgnysmgvreqiifpeidydkvdrvrgldititttaksdeegrallaafdfpfrk
    

  • Chain 'G':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tG (G:)
    srvakapvvvpagvdvkingqvitikgkngeltrtlndavevkhadntltfgprdgyadg
    waqagtarallnsmvigvtegftkklqlvgvgyraavkgnvinlslgfshpvdhqlpagi
    taecptqteivlkgadkqvigqvaadlrayrrpepykgkgvryadevvrtkeakkk
    

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tH (H:)
    mqvilldkvanlgslgdqvnvkagyarnflvpqgkavpatkknieffearraeleaklae
    vlaaanaraekinaletvtiaskagdegklfgsigtrdiadavtaagvevaksevrlpng
    vlrttgehevsfqvhsevfakvivnvvae
    

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tI (I:)
    akkvqayvklqvaagmanpsppvgpalgqqgvnimefckafnaktdsiekglpipvvitv
    yadrsftfvtktppaavllkkaagiksgsgkpnkdkvgkisraqlqeiaqtkaadmtgad
    ieamtrsiegtarsmglvved
    

  • Chain 'J':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tJ (J:)
    mktftakpetvkrdwyvvdatgktlgrlatelarrlrgkhkaeytphvdtgdyiivlnad
    kvavtgnkrtdkvyyhhtghiggikqatfeemiarrpervieiavkgmlpkgplgramfr
    klkvyagnehnhaaqqpqvldi
    

  • Chain 'K':
    Sequence, based on SEQRES records: (download)
    >2i2tK (K:)
    miqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlka
    vvvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislap
    evl
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tK (K:)
    iqeqtmlnvadnsgarrvmcikvlggshrryagvgdiikitikeaiprgkvkkgdvlkav
    vvrtkkgvrrpdgsvirfdgnacvllnnnseqpigtrifgpvtrelrsekfmkiislape
    v
    

  • Chain 'L':
    Sequence, based on SEQRES records: (download)
    >2i2tL (L:)
    mrlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrr
    lpkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpv
    tvrglrvtkgaraaieaaggkiee
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tL (L:)
    rlntlspaegskkagkrlgrgigsglgktggrghkgqksrsgggvrrgfeggqmplyrrl
    pkfgftsrkaaitaeirlsdlakveggvvdlntlkaaniigiqiefakvilagevttpvt
    vrglrvtkgaraaieaaggkiee
    

  • Chain 'M':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tM (M:)
    mlqpkrtkfrkmhkgrnrglaqgtdvsfgsfglkavgrgrltarqieaarramtravkrq
    gkiwirvfpdkpitekplavrmgkgkgnveywvaliqpgkvlyemdgvpeelareafkla
    aaklpikttfvtktvm
    

  • Chain 'N':
    Sequence, based on SEQRES records: (download)
    >2i2tN (N:)
    mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
    anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse
    kaeaaae
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tN (N:)
    mrhrksgrqlnrnsshrqamfrnmagslvrheiikttlpkakelrrvveplitlaktdsv
    anrrlafartrdneivaklfnelgprfasraggytrilkcgfragdnapmayielvdrse
    

  • Chain 'O':
    Sequence, based on SEQRES records: (download)
    >2i2tO (O:)
    mdkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiae
    qlkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tO (O:)
    dkksarirratrarrklqelgatrlvvhrtprhiyaqviapngsevlvaastvekaiaeq
    lkytgnkdaaaavgkavaeralekgikdvsfdrsgfqyhgrvqaladaareaglqf
    

  • Chain 'P':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tP (P:)
    sniikqleqeqmkqdvpsfrpgdtvevkvwvvegskkrlqafegvviairnrglhsaftv
    rkisngegvervfqthspvvdsisvkrrgavrkaklyylrertgkaarikerln
    

  • Chain 'Q':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tQ (Q:)
    arvkrgviararhkkilkqakgyygarsrvyrvafqavikagqyayrdrrqrkrqfrqlw
    iarinaaarqngisyskfinglkkasveidrkiladiavfdkvaftalvekakaala
    

  • Chain 'R':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tR (R:)
    myavfqsggkqhrvsegqtvrlekldiatgetvefaevlmiangeevkigvpfvdggvik
    aevvahgrgekvkivkfrrrkhyrkqqghrqwftdvkitgisa
    

  • Chain 'S':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tS (S:)
    metiakhrharssaqkvrlvadlirgkkvsqaldiltytnkkaavlvkkvlesaianaeh
    ndgadiddlkvtkifvdegpsmkrimprakgradrilkrtshitvvvsdr
    

  • Chain 'T':
    Sequence, based on SEQRES records: (download)
    >2i2tT (T:)
    mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
    lvvkgkvkrhgqrigrrsdwkkayvtlkegqnldfvggae
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tT (T:)
    mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevvnt
    lvvkgkvkrhgqrigrrsdwkkayvtlkegqnl
    

  • Chain 'U':
    Sequence, based on SEQRES records: (download)
    >2i2tU (U:)
    aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
    eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnsetik
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tU (U:)
    aakirrddevivltgkdkgkrgkvknvlssgkviveginlvkkhqkpvpalnqpggivek
    eaaiqvsnvaifnaatgkadrvgfrfedgkkvrffksnseti
    

  • Chain 'V':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tV (V:)
    mftinaevrkeqgkgasrrlraankfpaiiyggkeaplaieldhdkvmnmqakaefysev
    ltivvdgkeikvkaqdvqrhpykpklqhidfvra
    

  • Chain 'W':
    Sequence, based on SEQRES records: (download)
    >2i2tW (W:)
    ahkkaggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfa
    kadgkvkfevkgpknrkfisieae
    

    Sequence, based on observed residues (ATOM records): (download)
    >2i2tW (W:)
    ggstrngrdseakrlgvkrfggesvlagsiivrqrgtkfhaganvgcgrdhtlfakadgk
    vkfevkgpknrkfisieae
    

  • Chain 'X':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tX (X:)
    srvcqvtgkrpvtgnnrshalnatkrrflpnlhshrfwvesekrfvtlrvsakgmrvidk
    kgidtvlaelrargeky
    

  • Chain 'Y':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tY (Y:)
    mkakelreksveelntellnllreqfnlrmqaasgqlqqshllkqvrrdvarvktllnek
    aga
    

  • Chain 'Z':
    Sequence; same for both SEQRES and ATOM records: (download)
    >2i2tZ (Z:)
    aktikitqtrsaigrlpkhkatllglglrrightveredtpairgminavsfmvkvee